Molecular and physiological responses to abscisic acid and salts in roots of salt-sensitive and salt-tolerant Indica rice varieties
- Author
- Ann Moons (UGent) , Guy Bauw (UGent) , Els Prinsen, Marc Van Montagu (UGent) and Dominique Van Der Straeten (UGent)
- Organization
- Abstract
- The Indica rice (Oryza sativa L.) varieties Pokkali and Nona Bokra are well-known salt tolerance donors in classical breeding. In an attempt to understand the molecular basis of their tolerance, physiological and gene expression studies were initiated. The effect of abscisic acid (ABA) on total proteins in roots from 12-d-old seedlings of Pokkali, Nona Bokra, and the salt-sensitive cultivar Taichung N1 were analyzed on two-dimensional gels. The abundance of ABA-induced proteins was highest in the most tolerant variety, Pokkali. Three ABA-responsive proteins, present at different levels in roots from tolerant and sensitive varieties, were further characterized by partial amino acid analysis. A novel histidine-rich protein and two types of late embryogenesis abundant (LEA) proteins were identified. Protein immunoblotting revealed that the levels of dehydrins and group 3 LEA proteins were significantly higher in roots from tolerant compared with sensitive varieties. Endogenous ABA levels showed a transient increase in roots exposed to osmotic shock (150 mM NaCl). Peak ABA concentrations were 30-fold higher for Nona Bokra and 6-fold higher for Pokkali compared with Taichung N1. Both the salt-induced endogenous ABA levels and a greater molecular response of root tissue to ABA were associated with the varietal differences in tolerance.
- Keywords
- ORGAN-SPECIFIC EXPRESSION, PLANT CRATEROSTIGMA-PLANTAGINEUM, LOPHOPYRUM-ELONGATUM, DEHYDRATION STRESS, COLD-ACCLIMATION, GENE-EXPRESSION, INDUCED PROTEIN, ORYZA-SATIVA, LEA PROTEINS, WHEAT
Downloads
-
(...).pdf
- full text
- |
- UGent only
- |
- |
- 1.79 MB
Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-191839
- MLA
- Moons, Ann, et al. “Molecular and Physiological Responses to Abscisic Acid and Salts in Roots of Salt-Sensitive and Salt-Tolerant Indica Rice Varieties.” PLANT PHYSIOLOGY, vol. 107, no. 1, 1995, pp. 177–86, doi:10.1104/pp.107.1.177.
- APA
- Moons, A., Bauw, G., Prinsen, E., Van Montagu, M., & Van Der Straeten, D. (1995). Molecular and physiological responses to abscisic acid and salts in roots of salt-sensitive and salt-tolerant Indica rice varieties. PLANT PHYSIOLOGY, 107(1), 177–186. https://doi.org/10.1104/pp.107.1.177
- Chicago author-date
- Moons, Ann, Guy Bauw, Els Prinsen, Marc Van Montagu, and Dominique Van Der Straeten. 1995. “Molecular and Physiological Responses to Abscisic Acid and Salts in Roots of Salt-Sensitive and Salt-Tolerant Indica Rice Varieties.” PLANT PHYSIOLOGY 107 (1): 177–86. https://doi.org/10.1104/pp.107.1.177.
- Chicago author-date (all authors)
- Moons, Ann, Guy Bauw, Els Prinsen, Marc Van Montagu, and Dominique Van Der Straeten. 1995. “Molecular and Physiological Responses to Abscisic Acid and Salts in Roots of Salt-Sensitive and Salt-Tolerant Indica Rice Varieties.” PLANT PHYSIOLOGY 107 (1): 177–186. doi:10.1104/pp.107.1.177.
- Vancouver
- 1.Moons A, Bauw G, Prinsen E, Van Montagu M, Van Der Straeten D. Molecular and physiological responses to abscisic acid and salts in roots of salt-sensitive and salt-tolerant Indica rice varieties. PLANT PHYSIOLOGY. 1995;107(1):177–86.
- IEEE
- [1]A. Moons, G. Bauw, E. Prinsen, M. Van Montagu, and D. Van Der Straeten, “Molecular and physiological responses to abscisic acid and salts in roots of salt-sensitive and salt-tolerant Indica rice varieties,” PLANT PHYSIOLOGY, vol. 107, no. 1, pp. 177–186, 1995.
@article{191839, abstract = {{The Indica rice (Oryza sativa L.) varieties Pokkali and Nona Bokra are well-known salt tolerance donors in classical breeding. In an attempt to understand the molecular basis of their tolerance, physiological and gene expression studies were initiated. The effect of abscisic acid (ABA) on total proteins in roots from 12-d-old seedlings of Pokkali, Nona Bokra, and the salt-sensitive cultivar Taichung N1 were analyzed on two-dimensional gels. The abundance of ABA-induced proteins was highest in the most tolerant variety, Pokkali. Three ABA-responsive proteins, present at different levels in roots from tolerant and sensitive varieties, were further characterized by partial amino acid analysis. A novel histidine-rich protein and two types of late embryogenesis abundant (LEA) proteins were identified. Protein immunoblotting revealed that the levels of dehydrins and group 3 LEA proteins were significantly higher in roots from tolerant compared with sensitive varieties. Endogenous ABA levels showed a transient increase in roots exposed to osmotic shock (150 mM NaCl). Peak ABA concentrations were 30-fold higher for Nona Bokra and 6-fold higher for Pokkali compared with Taichung N1. Both the salt-induced endogenous ABA levels and a greater molecular response of root tissue to ABA were associated with the varietal differences in tolerance.}}, author = {{Moons, Ann and Bauw, Guy and Prinsen, Els and Van Montagu, Marc and Van Der Straeten, Dominique}}, issn = {{0032-0889}}, journal = {{PLANT PHYSIOLOGY}}, keywords = {{ORGAN-SPECIFIC EXPRESSION,PLANT CRATEROSTIGMA-PLANTAGINEUM,LOPHOPYRUM-ELONGATUM,DEHYDRATION STRESS,COLD-ACCLIMATION,GENE-EXPRESSION,INDUCED PROTEIN,ORYZA-SATIVA,LEA PROTEINS,WHEAT}}, language = {{eng}}, number = {{1}}, pages = {{177--186}}, title = {{Molecular and physiological responses to abscisic acid and salts in roots of salt-sensitive and salt-tolerant Indica rice varieties}}, url = {{http://doi.org/10.1104/pp.107.1.177}}, volume = {{107}}, year = {{1995}}, }
- Altmetric
- View in Altmetric
- Web of Science
- Times cited: