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Primary structure of a hormonally regulated β-glucanase of Nicotiana plumbaginifolia

(1988) GENE. 70(1). p.13-23
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Abstract
A cDNA clone for a hormonally regulated beta-glucanase from Nicotiana plumbaginifolia has been isolated by using an oligodeoxynucleotide probe, synthesized to match the previously determined N-terminal amino acid sequence. The cDNA has the complete sequence of the mature protein and contains at least part of a hydrophobic signal peptide. At the amino acid level, the beta-glucanase of N. plumbaginifolia is 73% homologous to a beta(1,3)-glucanase from tobacco and 52% homologous to a beta(1,3;1,4)-glucanase from barley. Southern-blot analysis clearly demonstrated that N. plumbaginifolia contains at least two related genes encoding beta-glucanase. The extent of the complete signal peptide of the cloned beta-glucanase was determined by sequencing part of the corresponding gene. Northern analysis showed that the expression of the beta-glucanase gene is influenced by auxins and cytokinins.

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Chicago
De Loose, Marc, Thierry Alliottte, Godelieve Gheysen, Christiane Genetello, Jan Gielen, P Soetaert, Marc Van Montagu, and Dirk Inzé. 1988. “Primary Structure of a Hormonally Regulated Β-glucanase of Nicotiana Plumbaginifolia.” Gene 70 (1): 13–23.
APA
De Loose, M., Alliottte, T., Gheysen, G., Genetello, C., Gielen, J., Soetaert, P., Van Montagu, M., et al. (1988). Primary structure of a hormonally regulated β-glucanase of Nicotiana plumbaginifolia. GENE, 70(1), 13–23.
Vancouver
1.
De Loose M, Alliottte T, Gheysen G, Genetello C, Gielen J, Soetaert P, et al. Primary structure of a hormonally regulated β-glucanase of Nicotiana plumbaginifolia. GENE. 1988;70(1):13–23.
MLA
De Loose, Marc, Thierry Alliottte, Godelieve Gheysen, et al. “Primary Structure of a Hormonally Regulated Β-glucanase of Nicotiana Plumbaginifolia.” GENE 70.1 (1988): 13–23. Print.
@article{1918017,
  abstract     = {A cDNA clone for a hormonally regulated beta-glucanase from Nicotiana plumbaginifolia has been isolated by using an oligodeoxynucleotide probe, synthesized to match the previously determined N-terminal amino acid sequence. The cDNA has the complete sequence of the mature protein and contains at least part of a hydrophobic signal peptide. At the amino acid level, the beta-glucanase of N. plumbaginifolia is 73\% homologous to a beta(1,3)-glucanase from tobacco and 52\% homologous to a beta(1,3;1,4)-glucanase from barley. Southern-blot analysis clearly demonstrated that N. plumbaginifolia contains at least two related genes encoding beta-glucanase. The extent of the complete signal peptide of the cloned beta-glucanase was determined by sequencing part of the corresponding gene. Northern analysis showed that the expression of the beta-glucanase gene is influenced by auxins and cytokinins.},
  author       = {De Loose, Marc and Alliottte, Thierry and Gheysen, Godelieve and Genetello, Christiane and Gielen, Jan and Soetaert, P and Van Montagu, Marc and Inz{\'e}, Dirk},
  issn         = {0378-1119},
  journal      = {GENE},
  language     = {eng},
  number       = {1},
  pages        = {13--23},
  title        = {Primary structure of a hormonally regulated \ensuremath{\beta}-glucanase of Nicotiana plumbaginifolia},
  url          = {http://dx.doi.org/10.1016/0378-1119(88)90100-X},
  volume       = {70},
  year         = {1988},
}

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