The primary structure of the basic isoform of Acanthamoeba profilin
- Author
- Christophe Ampe (UGent) , Masahiko Sato, Thomas D Pollard and Joël Vandekerckhove (UGent)
- Organization
- Abstract
- Acanthamoeba profilin-II [Kaiser, D.A., Sato, M., Ebert, R. F. and Pollard, T.D. (1986) J. Cell. Biol. 102, 221-226] was digested with trypsin or cleaved by 2-(2-nitrophenylsulphenyl)-3-methyl-3-bromoindolenine. The tryptic peptides were purified by reversed-phase-high-performance liquid chromatography and completely sequenced using automated gas-phase sequence analysis. The complete profilin-II sequence was deduced by ordering the tryptic peptides using the sequence information of the tryptophan-cleavage products. Acanthamoeba profilin-II was found to be homologous to the previously determined profilin-I sequence [Ampe, C., Vandekerckhove, J., Brenner, L., Tobacman, L. and Korn, E.D. (1985) J. Biol. Chem. 260, 834-840]. Like profilin-I, profilin-II consists of 125 amino acids, has a blocked NH2 terminus and a trimethyllysine residue at position 103. Profilin-II differs in at least 21 positions from one of the profilin-I isoforms. The amino acid exchanges are mainly concentrated in the middle part of the sequence. Profilin-II contains two more basic residues than profilin-I, which explains its higher isoelectric point.
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-1917790
- MLA
- Ampe, Christophe, et al. “The Primary Structure of the Basic Isoform of Acanthamoeba Profilin.” EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 170, no. 3, 1988, pp. 597–601, doi:10.1111/j.1432-1033.1988.tb13739.x.
- APA
- Ampe, C., Sato, M., Pollard, T. D., & Vandekerckhove, J. (1988). The primary structure of the basic isoform of Acanthamoeba profilin. EUROPEAN JOURNAL OF BIOCHEMISTRY, 170(3), 597–601. https://doi.org/10.1111/j.1432-1033.1988.tb13739.x
- Chicago author-date
- Ampe, Christophe, Masahiko Sato, Thomas D Pollard, and Joël Vandekerckhove. 1988. “The Primary Structure of the Basic Isoform of Acanthamoeba Profilin.” EUROPEAN JOURNAL OF BIOCHEMISTRY 170 (3): 597–601. https://doi.org/10.1111/j.1432-1033.1988.tb13739.x.
- Chicago author-date (all authors)
- Ampe, Christophe, Masahiko Sato, Thomas D Pollard, and Joël Vandekerckhove. 1988. “The Primary Structure of the Basic Isoform of Acanthamoeba Profilin.” EUROPEAN JOURNAL OF BIOCHEMISTRY 170 (3): 597–601. doi:10.1111/j.1432-1033.1988.tb13739.x.
- Vancouver
- 1.Ampe C, Sato M, Pollard TD, Vandekerckhove J. The primary structure of the basic isoform of Acanthamoeba profilin. EUROPEAN JOURNAL OF BIOCHEMISTRY. 1988;170(3):597–601.
- IEEE
- [1]C. Ampe, M. Sato, T. D. Pollard, and J. Vandekerckhove, “The primary structure of the basic isoform of Acanthamoeba profilin,” EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 170, no. 3, pp. 597–601, 1988.
@article{1917790,
abstract = {{Acanthamoeba profilin-II [Kaiser, D.A., Sato, M., Ebert, R. F. and Pollard, T.D. (1986) J. Cell. Biol. 102, 221-226] was digested with trypsin or cleaved by 2-(2-nitrophenylsulphenyl)-3-methyl-3-bromoindolenine. The tryptic peptides were purified by reversed-phase-high-performance liquid chromatography and completely sequenced using automated gas-phase sequence analysis. The complete profilin-II sequence was deduced by ordering the tryptic peptides using the sequence information of the tryptophan-cleavage products. Acanthamoeba profilin-II was found to be homologous to the previously determined profilin-I sequence [Ampe, C., Vandekerckhove, J., Brenner, L., Tobacman, L. and Korn, E.D. (1985) J. Biol. Chem. 260, 834-840]. Like profilin-I, profilin-II consists of 125 amino acids, has a blocked NH2 terminus and a trimethyllysine residue at position 103. Profilin-II differs in at least 21 positions from one of the profilin-I isoforms. The amino acid exchanges are mainly concentrated in the middle part of the sequence. Profilin-II contains two more basic residues than profilin-I, which explains its higher isoelectric point.}},
author = {{Ampe, Christophe and Sato, Masahiko and Pollard, Thomas D and Vandekerckhove, Joël}},
issn = {{0014-2956}},
journal = {{EUROPEAN JOURNAL OF BIOCHEMISTRY}},
language = {{eng}},
number = {{3}},
pages = {{597--601}},
title = {{The primary structure of the basic isoform of Acanthamoeba profilin}},
url = {{http://doi.org/10.1111/j.1432-1033.1988.tb13739.x}},
volume = {{170}},
year = {{1988}},
}
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