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cIAP1/2 are direct E3 ligases conjugating diverse types of ubiquitin chains to receptor interacting proteins kinases 1 to 4 (RIP1-4)

Mathieu Bertrand (UGent), Saskia Lippens (UGent), An Staes (UGent), Barbara Gilbert (UGent), Ria Roelandt (UGent), Jelle De Medts, Kris Gevaert (UGent), Wim Declercq (UGent) and Peter Vandenabeele (UGent)
(2011) PLOS ONE. 6(9).
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Ghent researchers on unfolded proteins in inflammatory disease (GROUP-ID)
Abstract
The RIP kinases have emerged as essential mediators of cellular stress that integrate both extracellular stimuli emanating from various cell-surface receptors and signals coming from intracellular pattern recognition receptors. The molecular mechanisms regulating the ability of the RIP proteins to transduce the stress signals remain poorly understood, but seem to rely only partially on their kinase activities. Recent studies on RIP1 and RIP2 have highlighted the importance of ubiquitination as a key process regulating their capacity to activate downstream signaling pathways. In this study, we found that XIAP, cIAP1 and cIAP2 not only directly bind to RIP1 and RIP2 but also to RIP3 and RIP4. We show that cIAP1 and cIAP2 are direct E3 ubiquitin ligases for all four RIP proteins and that cIAP1 is capable of conjugating the RIPs with diverse types of ubiquitin chains, including linear chains. Consistently, we show that repressing cIAP1/2 levels affects the activation of NF-kappa B that is dependent on RIP1, -2, -3 and -4. Finally, we identified Lys51 and Lys145 of RIP4 as two critical residues for cIAP1-mediated ubiquitination and NF-kB activation.
Keywords
C-ASSOCIATED KINASE, NF-KAPPA-B, TNF-ALPHA, SIGNALING COMPLEX, ACTIVATION, C-IAP1, NECROSIS, IMMUNE-RESPONSES, APOPTOSIS PROTEINS, CELL-DEATH

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Citation

Please use this url to cite or link to this publication:

Chicago
Bertrand, Mathieu, Saskia Lippens, An Staes, Barbara Gilbert, Ria Roelandt, Jelle De Medts, Kris Gevaert, Wim Declercq, and Peter Vandenabeele. 2011. “cIAP1/2 Are Direct E3 Ligases Conjugating Diverse Types of Ubiquitin Chains to Receptor Interacting Proteins Kinases 1 to 4 (RIP1-4).” Plos One 6 (9).
APA
Bertrand, Mathieu, Lippens, S., Staes, A., Gilbert, B., Roelandt, R., De Medts, J., Gevaert, K., et al. (2011). cIAP1/2 are direct E3 ligases conjugating diverse types of ubiquitin chains to receptor interacting proteins kinases 1 to 4 (RIP1-4). PLOS ONE, 6(9).
Vancouver
1.
Bertrand M, Lippens S, Staes A, Gilbert B, Roelandt R, De Medts J, et al. cIAP1/2 are direct E3 ligases conjugating diverse types of ubiquitin chains to receptor interacting proteins kinases 1 to 4 (RIP1-4). PLOS ONE. 2011;6(9).
MLA
Bertrand, Mathieu, Saskia Lippens, An Staes, et al. “cIAP1/2 Are Direct E3 Ligases Conjugating Diverse Types of Ubiquitin Chains to Receptor Interacting Proteins Kinases 1 to 4 (RIP1-4).” PLOS ONE 6.9 (2011): n. pag. Print.
@article{1917686,
  abstract     = {The RIP kinases have emerged as essential mediators of cellular stress that integrate both extracellular stimuli emanating from various cell-surface receptors and signals coming from intracellular pattern recognition receptors. The molecular mechanisms regulating the ability of the RIP proteins to transduce the stress signals remain poorly understood, but seem to rely only partially on their kinase activities. Recent studies on RIP1 and RIP2 have highlighted the importance of ubiquitination as a key process regulating their capacity to activate downstream signaling pathways. In this study, we found that XIAP, cIAP1 and cIAP2 not only directly bind to RIP1 and RIP2 but also to RIP3 and RIP4. We show that cIAP1 and cIAP2 are direct E3 ubiquitin ligases for all four RIP proteins and that cIAP1 is capable of conjugating the RIPs with diverse types of ubiquitin chains, including linear chains. Consistently, we show that repressing cIAP1/2 levels affects the activation of NF-kappa B that is dependent on RIP1, -2, -3 and -4. Finally, we identified Lys51 and Lys145 of RIP4 as two critical residues for cIAP1-mediated ubiquitination and NF-kB activation.},
  articleno    = {e22356},
  author       = {Bertrand, Mathieu and Lippens, Saskia and Staes, An and Gilbert, Barbara and Roelandt, Ria and De Medts, Jelle and Gevaert, Kris and Declercq, Wim and Vandenabeele, Peter},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keyword      = {C-ASSOCIATED KINASE,NF-KAPPA-B,TNF-ALPHA,SIGNALING COMPLEX,ACTIVATION,C-IAP1,NECROSIS,IMMUNE-RESPONSES,APOPTOSIS PROTEINS,CELL-DEATH},
  language     = {eng},
  number       = {9},
  pages        = {11},
  title        = {cIAP1/2 are direct E3 ligases conjugating diverse types of ubiquitin chains to receptor interacting proteins kinases 1 to 4 (RIP1-4)},
  url          = {http://dx.doi.org/10.1371/journal.pone.0022356},
  volume       = {6},
  year         = {2011},
}

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