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Extensin gene expression is induced by mechanical stimuli leading to local cell wall strengthening in Nicotiana plumbaginifolia

Christine Tiré (UGent) , Riet De Rycke (UGent) , Marc De Loose (UGent) , Dirk Inzé (UGent) , Marc Van Montagu (UGent) and Gilbert Engler (UGent)
(1994) PLANTA. 195(2). p.175-181
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Organization
Abstract
Nicotiana plumbaginifolia Viv. harbors a single extensin gene, although related hydroxyproline-rich sequences are present in the genome. Northern analysis showed that the gene is highly expressed in roots and to a lesser extent in stems. Expression in leaves is low but mRNA levels are increased upon infection with the incompatible bacterium Pseudomonas syringae. Extensin transcript levels in leaves were slightly enhanced after wounding and salicylic acid treatment. In-situ hybridization experiments showed high accumulation of extensin mRNA in cells which, at certain stages of development, require reinforcement of their cell walls. The cortical cells in stem nodes and roots, which are put under severe mechanical stress by adjacent developing tissues, tend to express the gene to high levels. Immunolocalization of the extensin protein in stems and roots demonstrated a close association of the protein with lignin deposition. Mature tissues contained more extensin than younger tissues. The extensin promoter was fused to the beta-glucuronidase gene.
Keywords
DEVELOPMENTAL REGULATION, EXTENSIN (GENE EXPRESSION), GENE EXPRESSION (EXTENSIN), NICOTIANA (EXTENSIN), PROTEIN LOCALIZATION, HYDROXYPROLINE-RICH GLYCOPROTEINS, ARABIDOPSIS 2S ALBUMIN, PROTEIN GENE, DIFFERENTIAL EXPRESSION, TRANSGENIC TOBACCO, VASCULAR SYSTEM, PLANTS, LOCALIZATION, FAMILY, ANTIBODY

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Chicago
Tiré, Christine, Riet De Rycke, Marc De Loose, Dirk Inzé, Marc Van Montagu, and Gilbert Engler. 1994. “Extensin Gene Expression Is Induced by Mechanical Stimuli Leading to Local Cell Wall Strengthening in Nicotiana Plumbaginifolia.” Planta 195 (2): 175–181.
APA
Tiré, C., De Rycke, R., De Loose, M., Inzé, D., Van Montagu, M., & Engler, G. (1994). Extensin gene expression is induced by mechanical stimuli leading to local cell wall strengthening in Nicotiana plumbaginifolia. PLANTA, 195(2), 175–181.
Vancouver
1.
Tiré C, De Rycke R, De Loose M, Inzé D, Van Montagu M, Engler G. Extensin gene expression is induced by mechanical stimuli leading to local cell wall strengthening in Nicotiana plumbaginifolia. PLANTA. 1994;195(2):175–81.
MLA
Tiré, Christine, Riet De Rycke, Marc De Loose, et al. “Extensin Gene Expression Is Induced by Mechanical Stimuli Leading to Local Cell Wall Strengthening in Nicotiana Plumbaginifolia.” PLANTA 195.2 (1994): 175–181. Print.
@article{191509,
  abstract     = {Nicotiana plumbaginifolia Viv. harbors a single extensin gene, although related hydroxyproline-rich sequences are present in the genome. Northern analysis showed that the gene is highly expressed in roots and to a lesser extent in stems. Expression in leaves is low but mRNA levels are increased upon infection with the incompatible bacterium Pseudomonas syringae. Extensin transcript levels in leaves were slightly enhanced after wounding and salicylic acid treatment. In-situ hybridization experiments showed high accumulation of extensin mRNA in cells which, at certain stages of development, require reinforcement of their cell walls. The cortical cells in stem nodes and roots, which are put under severe mechanical stress by adjacent developing tissues, tend to express the gene to high levels. Immunolocalization of the extensin protein in stems and roots demonstrated a close association of the protein with lignin deposition. Mature tissues contained more extensin than younger tissues. The extensin promoter was fused to the beta-glucuronidase gene.},
  author       = {Tir{\'e}, Christine and De Rycke, Riet and De Loose, Marc and Inz{\'e}, Dirk and Van Montagu, Marc and Engler, Gilbert},
  issn         = {0032-0935},
  journal      = {PLANTA},
  keyword      = {DEVELOPMENTAL REGULATION,EXTENSIN (GENE EXPRESSION),GENE EXPRESSION (EXTENSIN),NICOTIANA (EXTENSIN),PROTEIN LOCALIZATION,HYDROXYPROLINE-RICH GLYCOPROTEINS,ARABIDOPSIS 2S ALBUMIN,PROTEIN GENE,DIFFERENTIAL EXPRESSION,TRANSGENIC TOBACCO,VASCULAR SYSTEM,PLANTS,LOCALIZATION,FAMILY,ANTIBODY},
  language     = {eng},
  number       = {2},
  pages        = {175--181},
  title        = {Extensin gene expression is induced by mechanical stimuli leading to local cell wall strengthening in Nicotiana plumbaginifolia},
  url          = {http://dx.doi.org/10.1007/BF00199676},
  volume       = {195},
  year         = {1994},
}

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