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The human keratinocyte two-dimensional protein database (update 1994): towards an integrated approach to the study of cell proliferation, differentiation and skin-diseases

(1994) ELECTROPHORESIS. 15(11). p.1349-1458
Author
Organization
Abstract
The master two-dimensional (2-D) gel database of human keratinocytes currently lists 3087 cellular proteins (2168 isoelectric focusing, IEF; and 919 nonequilibrium pH gradient electrophoresis, NEPHGE), many of which correspond to posttranslational modifications. 890 polypeptides have been identified (protein name, organelle components, etc.) using one or a combination of procedures that include (i) comigration with known human proteins, (ii) 2-D gel immunoblotting using specific antibodies (iii) microsequencing of Coomassie Brilliant Blue stained proteins, (iv) mass spectrometry and (v) vaccinia virus expression of full length cDNAs. These are listed both in alphabetical order and with increasing SSP number, together with their M(r), pi, cellular localization and credit to the investigator(s) that aided in the identification. Furthermore, we list 239 microsequenced proteins recorded in the database. We also report a database of proteins recovered from the medium of noncultured, unfractionated keratinocytes. This database lists 398 polypeptides (309 IEF; 89 NEPHGE) of which 76 have been identified. The aim of the comprehensive databases is to gather, through a systematic study of keratinocytes, qualitative and quantitative information on proteins and their genes that may allow us to identify abnormal patterns of gene expression and, ultimately, to pinpoint signaling pathways and components affected in various skin diseases, cancer included.
Keywords
2-DIMENSIONAL GEL-ELECTROPHORESIS, AMA, LINK, EXPRESSION, PSORIATIC SKIN, SIGNALING PATHWAY, MOLECULAR-CLONING, DNA-SEQUENCE DATA

Citation

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Chicago
Celis, Julio E, Hanne Holm Rasmussen, Eydfinnur Olsen, Peder Madsen, Henrik Leffers, Bent Honoré, Kurt Dejgaard, et al. 1994. “The Human Keratinocyte Two-dimensional Protein Database (update 1994): Towards an Integrated Approach to the Study of Cell Proliferation, Differentiation and Skin-diseases.” Electrophoresis 15 (11): 1349–1458.
APA
Celis, J. E., Rasmussen, H. H., Olsen, E., Madsen, P., Leffers, H., Honoré, B., Dejgaard, K., et al. (1994). The human keratinocyte two-dimensional protein database (update 1994): towards an integrated approach to the study of cell proliferation, differentiation and skin-diseases. ELECTROPHORESIS, 15(11), 1349–1458.
Vancouver
1.
Celis JE, Rasmussen HH, Olsen E, Madsen P, Leffers H, Honoré B, et al. The human keratinocyte two-dimensional protein database (update 1994): towards an integrated approach to the study of cell proliferation, differentiation and skin-diseases. ELECTROPHORESIS. 1994;15(11):1349–458.
MLA
Celis, Julio E, Hanne Holm Rasmussen, Eydfinnur Olsen, et al. “The Human Keratinocyte Two-dimensional Protein Database (update 1994): Towards an Integrated Approach to the Study of Cell Proliferation, Differentiation and Skin-diseases.” ELECTROPHORESIS 15.11 (1994): 1349–1458. Print.
@article{191414,
  abstract     = {The master two-dimensional (2-D) gel database of human keratinocytes currently lists 3087 cellular proteins (2168 isoelectric focusing, IEF; and 919 nonequilibrium pH gradient electrophoresis, NEPHGE), many of which correspond to posttranslational modifications. 890 polypeptides have been identified (protein name, organelle components, etc.) using one or a combination of procedures that include (i) comigration with known human proteins, (ii) 2-D gel immunoblotting using specific antibodies (iii) microsequencing of Coomassie Brilliant Blue stained proteins, (iv) mass spectrometry and (v) vaccinia virus expression of full length cDNAs. These are listed both in alphabetical order and with increasing SSP number, together with their M(r), pi, cellular localization and credit to the investigator(s) that aided in the identification. Furthermore, we list 239 microsequenced proteins recorded in the database. We also report a database of proteins recovered from the medium of noncultured, unfractionated keratinocytes. This database lists 398 polypeptides (309 IEF; 89 NEPHGE) of which 76 have been identified. The aim of the comprehensive databases is to gather, through a systematic study of keratinocytes, qualitative and quantitative information on proteins and their genes that may allow us to identify abnormal patterns of gene expression and, ultimately, to pinpoint signaling pathways and components affected in various skin diseases, cancer included.},
  author       = {Celis, Julio E and Rasmussen, Hanne Holm and Olsen, Eydfinnur and Madsen, Peder and Leffers, Henrik and Honor{\'e}, Bent and Dejgaard, Kurt and Gromov, Paul and Vorum, Henrik and Vassilev, Alex and Baskin, Yasemin and Liu, Xiangdang and Celis, Ariana and Basse, Bodil and Lauridsen, Jette B and Ratz, Gitte P and Andersen, Annette H and Walbum, Else and Kj{\ae}rgaard, Inge and Andersen, Inger and Puype, Magda and Van Damme, Jozef and Vandekerckhove, Jo{\"e}l},
  issn         = {0173-0835},
  journal      = {ELECTROPHORESIS},
  keyword      = {2-DIMENSIONAL GEL-ELECTROPHORESIS,AMA,LINK,EXPRESSION,PSORIATIC SKIN,SIGNALING PATHWAY,MOLECULAR-CLONING,DNA-SEQUENCE DATA},
  language     = {eng},
  number       = {11},
  pages        = {1349--1458},
  title        = {The human keratinocyte two-dimensional protein database (update 1994): towards an integrated approach to the study of cell proliferation, differentiation and skin-diseases},
  url          = {http://dx.doi.org/10.1002/elps.11501501208},
  volume       = {15},
  year         = {1994},
}

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