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Enhancement of oxidative stress tolerance in transgenic tobacco plants overproducing Fe-superoxide dismutase in chloroplasts

(1996) PLANT PHYSIOLOGY. 112(4). p.1703-1714
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Abstract
A chimeric gene consisting of the coding sequence for chloroplastic Fe superoxide dismutase (FeSOD) from Arabidopsis thaliana, coupled to the chloroplast targeting sequence from the pea ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit, was expressed in Nicotiana tabacum cv Petit Havana SR1. Expression of the transgenic FeSOD protected both the plasmalemma and photosystem II against superoxide generated during illumination of leaf discs impregnated with methyl viologen. By contrast, overproduction of a mitochondrial MnSOD from Nicotiana plumbaginifolia in the chloroplasts of cv SR1 protected only the plasmalemma, but not photosystem II, against methyl viologen (L. Slooten, K. Capiau, W. Van Camp, M. Van Montagu, C.Sybesma, D. Inze [1995] Plant Physiol 107: 737-750). The difference in effectiveness correlates with different membrane affinities of the transgenic FeSOD and MnSOD. Overproduction of FeSOD does not confer tolerance to H2O2, singlet oxygen, chilling-induced photoinhibition in leaf disc assays, or to salt stress at the whole plant level. In nontransgenic plants, salt stress led to a 2- to 3-fold increase in activity, on a protein basis, of FeSOD, cytosolic and chloroplastic Cu/ZnSOD, ascorbate peroxidase, dehydroascorbate reductase, and glutathione reductase. In FeSOD-overproducing plants under salt stress, the induction of cytosolic and chloroplastic Cu/ZnSOD was suppressed, whereas induction of a water-soluble chloroplastic ascorbate peroxidase isozyme was promoted.
Keywords
ESCHERICHIA-COLI, INTACT CHLOROPLASTS, OXYGEN RADICALS, ELEVATED LEVELS, SALT-TOLERANT, PISUM-SATIVUM, SPINACH-CHLOROPLASTS, HYDROGEN-PEROXIDE, ASCORBATE PEROXIDASE, PHOTOSYSTEM-II

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Citation

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Chicago
Van Camp, Wim, Katelijne Capiau, Marc Van Montagu, Dirk Inzé, and Luit Slooten. 1996. “Enhancement of Oxidative Stress Tolerance in Transgenic Tobacco Plants Overproducing Fe-superoxide Dismutase in Chloroplasts.” Plant Physiology 112 (4): 1703–1714.
APA
Van Camp, Wim, Capiau, K., Van Montagu, M., Inzé, D., & Slooten, L. (1996). Enhancement of oxidative stress tolerance in transgenic tobacco plants overproducing Fe-superoxide dismutase in chloroplasts. PLANT PHYSIOLOGY, 112(4), 1703–1714.
Vancouver
1.
Van Camp W, Capiau K, Van Montagu M, Inzé D, Slooten L. Enhancement of oxidative stress tolerance in transgenic tobacco plants overproducing Fe-superoxide dismutase in chloroplasts. PLANT PHYSIOLOGY. 1996;112(4):1703–14.
MLA
Van Camp, Wim, Katelijne Capiau, Marc Van Montagu, et al. “Enhancement of Oxidative Stress Tolerance in Transgenic Tobacco Plants Overproducing Fe-superoxide Dismutase in Chloroplasts.” PLANT PHYSIOLOGY 112.4 (1996): 1703–1714. Print.
@article{191357,
  abstract     = {A chimeric gene consisting of the coding sequence for chloroplastic Fe superoxide dismutase (FeSOD) from Arabidopsis thaliana, coupled to the chloroplast targeting sequence from the pea ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit, was expressed in Nicotiana tabacum cv Petit Havana SR1. Expression of the transgenic FeSOD protected both the plasmalemma and photosystem II against superoxide generated during illumination of leaf discs impregnated with methyl viologen. By contrast, overproduction of a mitochondrial MnSOD from Nicotiana plumbaginifolia in the chloroplasts of cv SR1 protected only the plasmalemma, but not photosystem II, against methyl viologen (L. Slooten, K. Capiau, W. Van Camp, M. Van Montagu, C.Sybesma, D. Inze [1995] Plant Physiol 107: 737-750). The difference in effectiveness correlates with different membrane affinities of the transgenic FeSOD and MnSOD. Overproduction of FeSOD does not confer tolerance to H2O2, singlet oxygen, chilling-induced photoinhibition in leaf disc assays, or to salt stress at the whole plant level. In nontransgenic plants, salt stress led to a 2- to 3-fold increase in activity, on a protein basis, of FeSOD, cytosolic and chloroplastic Cu/ZnSOD, ascorbate peroxidase, dehydroascorbate reductase, and glutathione reductase. In FeSOD-overproducing plants under salt stress, the induction of cytosolic and chloroplastic Cu/ZnSOD was suppressed, whereas induction of a water-soluble chloroplastic ascorbate peroxidase isozyme was promoted.},
  author       = {Van Camp, Wim and Capiau, Katelijne and Van Montagu, Marc and Inz{\'e}, Dirk and Slooten, Luit},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  keyword      = {ESCHERICHIA-COLI,INTACT CHLOROPLASTS,OXYGEN RADICALS,ELEVATED LEVELS,SALT-TOLERANT,PISUM-SATIVUM,SPINACH-CHLOROPLASTS,HYDROGEN-PEROXIDE,ASCORBATE PEROXIDASE,PHOTOSYSTEM-II},
  language     = {eng},
  number       = {4},
  pages        = {1703--1714},
  title        = {Enhancement of oxidative stress tolerance in transgenic tobacco plants overproducing Fe-superoxide dismutase in chloroplasts},
  url          = {http://dx.doi.org/10.1104/pp.112.4.1703},
  volume       = {112},
  year         = {1996},
}

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