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Chromosome landing at the Arabidopsis TORNADO1 locus using an AFLP-based strategy

(1996) MOLECULAR AND GENERAL GENETICS. 253(1-2). p.32-41
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Organization
Abstract
The Arabidopsis tornadol (trn1) mutation causes severe dwarfism combined with twisted growth of all organs. We present a chromosome landing strategy, using amplified restriction fragment length polymorphism (AFLP) marker technology, for the isolation of the TRN1 gene. The recessive trn1 mutation was identified in a C24 transgenic line and is located 5 cM from a T-DNA insertion. We mapped the TRN1 locus to the bottom half of chromosome 5 relative to visible and restriction fragment length polymorphism (RFLP) markers. Recombinant classes within a 3-cM region around TRN1 were used to build a high-resolution map in this region, using the AFLP technique. Approximately 300 primer combinations have been used to test about 26000 fragments for polymorphisms, Seventeen of these AFLP markers were identified in the 3-cM region around TRN1. These markers were mapped within this region using individual recombinants. Four of these AFLP markers co-segregate with TRN1 whereas one maps at one recombinant below TRN1. We isolated and cloned three of these AFLP markers. These markers identified two yeast artificial chromosome (YAC) clones, containing the RFLP marker above and the AFLP marker below TRN1, demonstrating that these YACs span the TRN1 locus and that chromosome landing has been achieved, using an AFLP-based strategy.
Keywords
Arabidopsis thaliana, AFLP markers, chromosome landing, physical mapping, tornadol, POLYMORPHISM LINKAGE MAP, HIGH-RESOLUTION MAP, THALIANA GENOME, YAC LIBRARY, GENE, DNA, MARKERS, CLONES, CONSTRUCTION, RESISTANCE

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Chicago
Cnops, Gerda, B den Boer, Tom Gerats, Marc Van Montagu, and Maria Van Lijsebettens. 1996. “Chromosome Landing at the Arabidopsis TORNADO1 Locus Using an AFLP-based Strategy.” Molecular and General Genetics 253 (1-2): 32–41.
APA
Cnops, G., den Boer, B., Gerats, T., Van Montagu, M., & Van Lijsebettens, M. (1996). Chromosome landing at the Arabidopsis TORNADO1 locus using an AFLP-based strategy. MOLECULAR AND GENERAL GENETICS, 253(1-2), 32–41.
Vancouver
1.
Cnops G, den Boer B, Gerats T, Van Montagu M, Van Lijsebettens M. Chromosome landing at the Arabidopsis TORNADO1 locus using an AFLP-based strategy. MOLECULAR AND GENERAL GENETICS. 1996;253(1-2):32–41.
MLA
Cnops, Gerda, B den Boer, Tom Gerats, et al. “Chromosome Landing at the Arabidopsis TORNADO1 Locus Using an AFLP-based Strategy.” MOLECULAR AND GENERAL GENETICS 253.1-2 (1996): 32–41. Print.
@article{191302,
  abstract     = {The Arabidopsis tornadol (trn1) mutation causes severe dwarfism combined with twisted growth of all organs. We present a chromosome landing strategy, using amplified restriction fragment length polymorphism (AFLP) marker technology, for the isolation of the TRN1 gene. The recessive trn1 mutation was identified in a C24 transgenic line and is located 5 cM from a T-DNA insertion. We mapped the TRN1 locus to the bottom half of chromosome 5 relative to visible and restriction fragment length polymorphism (RFLP) markers. Recombinant classes within a 3-cM region around TRN1 were used to build a high-resolution map in this region, using the AFLP technique. Approximately 300 primer combinations have been used to test about 26000 fragments for polymorphisms, Seventeen of these AFLP markers were identified in the 3-cM region around TRN1. These markers were mapped within this region using individual recombinants. Four of these AFLP markers co-segregate with TRN1 whereas one maps at one recombinant below TRN1. We isolated and cloned three of these AFLP markers. These markers identified two yeast artificial chromosome (YAC) clones, containing the RFLP marker above and the AFLP marker below TRN1, demonstrating that these YACs span the TRN1 locus and that chromosome landing has been achieved, using an AFLP-based strategy.},
  author       = {Cnops, Gerda and den Boer, B and Gerats, Tom and Van Montagu, Marc and Van Lijsebettens, Maria},
  issn         = {0026-8925},
  journal      = {MOLECULAR AND GENERAL GENETICS},
  keyword      = {Arabidopsis thaliana,AFLP markers,chromosome landing,physical mapping,tornadol,POLYMORPHISM LINKAGE MAP,HIGH-RESOLUTION MAP,THALIANA GENOME,YAC LIBRARY,GENE,DNA,MARKERS,CLONES,CONSTRUCTION,RESISTANCE},
  language     = {eng},
  number       = {1-2},
  pages        = {32--41},
  title        = {Chromosome landing at the Arabidopsis TORNADO1 locus using an AFLP-based strategy},
  url          = {http://dx.doi.org/10.1007/s004380050293},
  volume       = {253},
  year         = {1996},
}

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