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Characterization of vacuolar and extracellular β(1,3)-glucanases of tobacco: evidence for a strictly compartmentalized plant defense system

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Abstract
beta(1,3)-Glucanases are a class of hormonally and developmentally regulated plant hydrolytic enzymes, which are also induced upon pathogen infection, suggesting a role of these hydrolases in the defense response to pathogens. We have purified several beta(1,3)-glucanases present in tobacco leaves from control plants and from plants treated with salicylic acid or infected with Pseudomonas syringae and studied in detail the subcellular localization of the different isoforms. Partial protein sequence analysis demonstrated that each of the different isoforms had a unique amino acid sequence and was therefore encoded by a different gene. We have also demonstrated that two of these isoforms, similar to the cytokinin/auxin-regulated isoforms previously isolated from tobacco cell suspensions, are located in the central vacuole. Upon salicylic acid treatment or P. syringae infection, three secreted isoforms are induced, belonging to the so-called pathogenesis-related proteins. These pathogenesis-related beta(1,3)-glucanases are all distinct from each other and also different from the vacuolar isoforms. We demonstrate that the vacuolar isoforms are not secreted to the extracellular spaces of the plant following pathogen infection, suggesting that any function they play in the plant defense response is restricted to an intracellularly coordinated defense process.

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Chicago
Van Den Bulcke, Marc, Guy Bauw, C Castresana, Marc Van Montagu, and Joël Vandekerckhove. 1989. “Characterization of Vacuolar and Extracellular Β(1,3)-glucanases of Tobacco: Evidence for a Strictly Compartmentalized Plant Defense System.” Proceedings of the National Academy of Sciences of the United States of America 86 (8): 2673–2677.
APA
Van Den Bulcke, M., Bauw, G., Castresana, C., Van Montagu, M., & Vandekerckhove, J. (1989). Characterization of vacuolar and extracellular β(1,3)-glucanases of tobacco: evidence for a strictly compartmentalized plant defense system. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 86(8), 2673–2677.
Vancouver
1.
Van Den Bulcke M, Bauw G, Castresana C, Van Montagu M, Vandekerckhove J. Characterization of vacuolar and extracellular β(1,3)-glucanases of tobacco: evidence for a strictly compartmentalized plant defense system. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 1989;86(8):2673–7.
MLA
Van Den Bulcke, Marc, Guy Bauw, C Castresana, et al. “Characterization of Vacuolar and Extracellular Β(1,3)-glucanases of Tobacco: Evidence for a Strictly Compartmentalized Plant Defense System.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 86.8 (1989): 2673–2677. Print.
@article{1910372,
  abstract     = {beta(1,3)-Glucanases are a class of hormonally and developmentally regulated plant hydrolytic enzymes, which are also induced upon pathogen infection, suggesting a role of these hydrolases in the defense response to pathogens. We have purified several beta(1,3)-glucanases present in tobacco leaves from control plants and from plants treated with salicylic acid or infected with Pseudomonas syringae and studied in detail the subcellular localization of the different isoforms. Partial protein sequence analysis demonstrated that each of the different isoforms had a unique amino acid sequence and was therefore encoded by a different gene. We have also demonstrated that two of these isoforms, similar to the cytokinin/auxin-regulated isoforms previously isolated from tobacco cell suspensions, are located in the central vacuole. Upon salicylic acid treatment or P. syringae infection, three secreted isoforms are induced, belonging to the so-called pathogenesis-related proteins. These pathogenesis-related beta(1,3)-glucanases are all distinct from each other and also different from the vacuolar isoforms. We demonstrate that the vacuolar isoforms are not secreted to the extracellular spaces of the plant following pathogen infection, suggesting that any function they play in the plant defense response is restricted to an intracellularly coordinated defense process.},
  author       = {Van Den Bulcke, Marc and Bauw, Guy and Castresana, C and Van Montagu, Marc and Vandekerckhove, Jo{\"e}l},
  issn         = {0027-8424},
  journal      = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA},
  language     = {eng},
  number       = {8},
  pages        = {2673--2677},
  title        = {Characterization of vacuolar and extracellular \ensuremath{\beta}(1,3)-glucanases of tobacco: evidence for a strictly compartmentalized plant defense system},
  url          = {http://www.pnas.org/content/86/8/2673.short},
  volume       = {86},
  year         = {1989},
}