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Characterization of a gram-positive broad-host-range plasmid isolated from Lactobacillus hilgardii

Katty Josson, Trees Scheirlinck, Frank Michiels, Christ Platteeuw, Patrick Stanssens, Henk Joos, Patrick Dhaese UGent, Marc Zabeau and Jacques Mahillon (1989) PLASMID. 21(1). p.9-20
abstract
Two plasmids, pLAB1000 and pLAB2000 (3.3 and 9.1 kb, respectively), have been isolated from a grass silage strain of Lactobacillus hilgardii. Both plasmids were cloned in Escherichia coli and characterized through restriction mapping. A 1.6-kb XbaI-SacI fragment of pLAB1000 appeared to be sufficient for autonomous replication in Lactobacillus plantarum and in Bacillus subtilis. Different shuttle vectors for E. coli and gram-positive bacteria were developed using the pLAB1000 plasmid. These could stably be maintained in Lactobacillus, Enterococcus, and Bacillus under selective conditions. Plasmids sharing DNA homologies with pLAB1000 have been observed in different strains of the related species L. plantarum.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
journal title
PLASMID
Plasmid
volume
21
issue
1
pages
9 - 20
Web of Science type
Article
ISSN
0147-619X
DOI
10.1016/0147-619X(89)90082-6
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1910075
handle
http://hdl.handle.net/1854/LU-1910075
date created
2011-09-28 10:20:48
date last changed
2016-12-19 15:46:25
@article{1910075,
  abstract     = {Two plasmids, pLAB1000 and pLAB2000 (3.3 and 9.1 kb, respectively), have been isolated from a grass silage strain of Lactobacillus hilgardii. Both plasmids were cloned in Escherichia coli and characterized through restriction mapping. A 1.6-kb XbaI-SacI fragment of pLAB1000 appeared to be sufficient for autonomous replication in Lactobacillus plantarum and in Bacillus subtilis. Different shuttle vectors for E. coli and gram-positive bacteria were developed using the pLAB1000 plasmid. These could stably be maintained in Lactobacillus, Enterococcus, and Bacillus under selective conditions. Plasmids sharing DNA homologies with pLAB1000 have been observed in different strains of the related species L. plantarum.},
  author       = {Josson, Katty and Scheirlinck, Trees and Michiels, Frank and Platteeuw, Christ and Stanssens, Patrick and Joos, Henk and Dhaese, Patrick and Zabeau, Marc and Mahillon, Jacques},
  issn         = {0147-619X},
  journal      = {PLASMID},
  language     = {eng},
  number       = {1},
  pages        = {9--20},
  title        = {Characterization of a gram-positive broad-host-range plasmid isolated from Lactobacillus hilgardii},
  url          = {http://dx.doi.org/10.1016/0147-619X(89)90082-6},
  volume       = {21},
  year         = {1989},
}

Chicago
Josson, Katty, Trees Scheirlinck, Frank Michiels, Christ Platteeuw, Patrick Stanssens, Henk Joos, Patrick Dhaese, Marc Zabeau, and Jacques Mahillon. 1989. “Characterization of a Gram-positive Broad-host-range Plasmid Isolated from Lactobacillus Hilgardii.” Plasmid 21 (1): 9–20.
APA
Josson, K., Scheirlinck, T., Michiels, F., Platteeuw, C., Stanssens, P., Joos, H., Dhaese, P., et al. (1989). Characterization of a gram-positive broad-host-range plasmid isolated from Lactobacillus hilgardii. PLASMID, 21(1), 9–20.
Vancouver
1.
Josson K, Scheirlinck T, Michiels F, Platteeuw C, Stanssens P, Joos H, et al. Characterization of a gram-positive broad-host-range plasmid isolated from Lactobacillus hilgardii. PLASMID. 1989;21(1):9–20.
MLA
Josson, Katty, Trees Scheirlinck, Frank Michiels, et al. “Characterization of a Gram-positive Broad-host-range Plasmid Isolated from Lactobacillus Hilgardii.” PLASMID 21.1 (1989): 9–20. Print.