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Common nodABC genes in Nod locus 1 of Azorhizobium caulinodans: nucleotide sequence and plant-inducible expression

Koen Goethals (UGent) , Mengsheng Gao (UGent) , K Tomekpe, Marc Van Montagu (UGent) and Marcella Holsters (UGent)
(1989) MOLECULAR & GENERAL GENETICS. 219(1-2). p.289-298
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Abstract
Azorhizobium caulinodans strain ORS571 induces nitrogen-fixing nodules on roots and stem-located root primordia of Sesbania rostrata. Two essential Nod loci have been previously identified in the bacterial genome, one of which (Nod locus 1) shows weak homology with the common nodC gene of Rhizobium meliloti. Here we present the nucleotide sequence of this region and show that it contains three contiguous open reading frames (ORFA, ORFB and ORFC) that are related to the nodABC genes of Rhizobium and Bradyrhizobium species. ORFC is followed by a fourth (ORF4) and probably a fifth (ORF5) open reading frame. ORF4 may be analogous to the nodI gene of R. leguminosarum, whereas ORF5 could be similar to the rhizobial nodF genes. Coordinated expression of this set of five genes seems likely from the sequence organization. There is no typical nod promoter consensus sequence (nod box) in the region upstream of the first gene (ORFA) and there is no nodD-like gene. LacZ fusions constructed with ORFA, ORFB, ORFC, and ORF4 showed inducible beta-galactosidase expression in the presence of S. rostrata seedlings as well as around stem-located root primordia. Among a series of phenolic compounds tested, the flavanone naringenin was the most efficient inducer of the expression of this ORS571 nod gene cluster.

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Chicago
Goethals, Koen, Mengsheng Gao, K Tomekpe, Marc Van Montagu, and Marcella Holsters. 1989. “Common nodABC Genes in Nod Locus 1 of Azorhizobium Caulinodans: Nucleotide Sequence and Plant-inducible Expression.” Molecular & General Genetics 219 (1-2): 289–298.
APA
Goethals, Koen, Gao, M., Tomekpe, K., Van Montagu, M., & Holsters, M. (1989). Common nodABC genes in Nod locus 1 of Azorhizobium caulinodans: nucleotide sequence and plant-inducible expression. MOLECULAR & GENERAL GENETICS, 219(1-2), 289–298.
Vancouver
1.
Goethals K, Gao M, Tomekpe K, Van Montagu M, Holsters M. Common nodABC genes in Nod locus 1 of Azorhizobium caulinodans: nucleotide sequence and plant-inducible expression. MOLECULAR & GENERAL GENETICS. 1989;219(1-2):289–98.
MLA
Goethals, Koen, Mengsheng Gao, K Tomekpe, et al. “Common nodABC Genes in Nod Locus 1 of Azorhizobium Caulinodans: Nucleotide Sequence and Plant-inducible Expression.” MOLECULAR & GENERAL GENETICS 219.1-2 (1989): 289–298. Print.
@article{1909922,
  abstract     = {Azorhizobium caulinodans strain ORS571 induces nitrogen-fixing nodules on roots and stem-located root primordia of Sesbania rostrata. Two essential Nod loci have been previously identified in the bacterial genome, one of which (Nod locus 1) shows weak homology with the common nodC gene of Rhizobium meliloti. Here we present the nucleotide sequence of this region and show that it contains three contiguous open reading frames (ORFA, ORFB and ORFC) that are related to the nodABC genes of Rhizobium and Bradyrhizobium species. ORFC is followed by a fourth (ORF4) and probably a fifth (ORF5) open reading frame. ORF4 may be analogous to the nodI gene of R. leguminosarum, whereas ORF5 could be similar to the rhizobial nodF genes. Coordinated expression of this set of five genes seems likely from the sequence organization. There is no typical nod promoter consensus sequence (nod box) in the region upstream of the first gene (ORFA) and there is no nodD-like gene. LacZ fusions constructed with ORFA, ORFB, ORFC, and ORF4 showed inducible beta-galactosidase expression in the presence of S. rostrata seedlings as well as around stem-located root primordia. Among a series of phenolic compounds tested, the flavanone naringenin was the most efficient inducer of the expression of this ORS571 nod gene cluster.},
  author       = {Goethals, Koen and Gao, Mengsheng and Tomekpe, K and Van Montagu, Marc and Holsters, Marcella},
  issn         = {0026-8925},
  journal      = {MOLECULAR \& GENERAL GENETICS},
  language     = {eng},
  number       = {1-2},
  pages        = {289--298},
  title        = {Common nodABC genes in Nod locus 1 of Azorhizobium caulinodans: nucleotide sequence and plant-inducible expression},
  url          = {http://dx.doi.org/10.1007/BF00261190},
  volume       = {219},
  year         = {1989},
}

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