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Biodegradation of poly(3-hydroxyalkanoates) in anaerobic sludge and characterization of a poly(3-hydroxyalkanoates) degrading anaerobic bacterium

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Abstract
The degradation of samples of poly(3-hydroxybutyrate) [P(3HB)], copolymers of 3-hydroxybutyrate and 10 and 20% 3-hydroxyvalerate [P(3HB-co-3HV)], and poly(3-hydroxyoctanoate-co-3-hydroxydecanoate) [P(3HO-co-3HD)] in anaerobic sludge was investigated by gravimetry, changes in molecular weight and mechanical properties. After 123 days, the mass of P(3HB) decreased by 15%. P(3HB-co-3HV) showed 7 and 11% mass loss, while P(3HO-co-3HD) showed no change in mass. The average molecular weight of all samples decreased with incubation rime bur this decrease was not correlated to the loss of mass, and is to be attributed to abiotic hydrolysis. The tensile strength of the P(3HB) and P(3HB-co-3HV) samples decreased with incubation time, but elongation remained practically unchanged. By enumeration, it was shown that anaerobic P(3HB) degrading bacteria were enriched during the incubation period. An anaerobic bacterium LMG 16094, able to degrade P(3HB) and P(3HB-co-19% 3HV) in pure culture, but not P(3HB-co-97% 3HV), P(3HO-co-3HD) or poly(epsilon-caprolactone), was isolated and characterized by fatty acid analysis. Its cells contained mainly straight chain, saturated and unsaturated fatty acids, with a high portion of fatty acids with 16 carbons, and did not contain hydroxylated or branched fatty acids. Its 16 rDNA gene sequence was found to be related to those of members of Clostridium group I (Collins er al., 1994). In a simple in vitro test using strain LMG 16094 in pure culture, an extruded P(3HB-co-12% 3HV) film lost 10% of its initial mass within 21 days of anaerobic incubation.
Keywords
poly(3-hydroxyalkanoates), biodegradation, poly(3-hydroxybutyrate), poly(3-hydroxybutyrate-co-3-hydroxyvalerate), poly(3-hydroxyoctanoate-co-3-hydroxydecanoate), anaerobic sludge, Clostridium, ILYOBACTER-DELAFIELDII, DEGRADATION, POLYHYDROXYALKANOATES, TREES

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MLA
Mergaert, Joris, Griet Glorieux, Lysiane Hauben, et al. “Biodegradation of Poly(3-hydroxyalkanoates) in Anaerobic Sludge and Characterization of a Poly(3-hydroxyalkanoates) Degrading Anaerobic Bacterium.” SYSTEMATIC AND APPLIED MICROBIOLOGY 19.3 (1996): 407–413. Print.
APA
Mergaert, J., Glorieux, G., Hauben, L., Storms, V., Mau, M., & Swings, J. (1996). Biodegradation of poly(3-hydroxyalkanoates) in anaerobic sludge and characterization of a poly(3-hydroxyalkanoates) degrading anaerobic bacterium. SYSTEMATIC AND APPLIED MICROBIOLOGY, 19(3), 407–413.
Chicago author-date
Mergaert, Joris, Griet Glorieux, Lysiane Hauben, Virginie Storms, Margit Mau, and Jean Swings. 1996. “Biodegradation of Poly(3-hydroxyalkanoates) in Anaerobic Sludge and Characterization of a Poly(3-hydroxyalkanoates) Degrading Anaerobic Bacterium.” Systematic and Applied Microbiology 19 (3): 407–413.
Chicago author-date (all authors)
Mergaert, Joris, Griet Glorieux, Lysiane Hauben, Virginie Storms, Margit Mau, and Jean Swings. 1996. “Biodegradation of Poly(3-hydroxyalkanoates) in Anaerobic Sludge and Characterization of a Poly(3-hydroxyalkanoates) Degrading Anaerobic Bacterium.” Systematic and Applied Microbiology 19 (3): 407–413.
Vancouver
1.
Mergaert J, Glorieux G, Hauben L, Storms V, Mau M, Swings J. Biodegradation of poly(3-hydroxyalkanoates) in anaerobic sludge and characterization of a poly(3-hydroxyalkanoates) degrading anaerobic bacterium. SYSTEMATIC AND APPLIED MICROBIOLOGY. 1996;19(3):407–13.
IEEE
[1]
J. Mergaert, G. Glorieux, L. Hauben, V. Storms, M. Mau, and J. Swings, “Biodegradation of poly(3-hydroxyalkanoates) in anaerobic sludge and characterization of a poly(3-hydroxyalkanoates) degrading anaerobic bacterium,” SYSTEMATIC AND APPLIED MICROBIOLOGY, vol. 19, no. 3, pp. 407–413, 1996.
@article{190946,
  abstract     = {{The degradation of samples of poly(3-hydroxybutyrate) [P(3HB)], copolymers of 3-hydroxybutyrate and 10 and 20% 3-hydroxyvalerate [P(3HB-co-3HV)], and poly(3-hydroxyoctanoate-co-3-hydroxydecanoate) [P(3HO-co-3HD)] in anaerobic sludge was investigated by gravimetry, changes in molecular weight and mechanical properties. After 123 days, the mass of P(3HB) decreased by 15%. P(3HB-co-3HV) showed 7 and 11% mass loss, while P(3HO-co-3HD) showed no change in mass. The average molecular weight of all samples decreased with incubation rime bur this decrease was not correlated to the loss of mass, and is to be attributed to abiotic hydrolysis. The tensile strength of the P(3HB) and P(3HB-co-3HV) samples decreased with incubation time, but elongation remained practically unchanged. By enumeration, it was shown that anaerobic P(3HB) degrading bacteria were enriched during the incubation period. An anaerobic bacterium LMG 16094, able to degrade P(3HB) and P(3HB-co-19% 3HV) in pure culture, but not P(3HB-co-97% 3HV), P(3HO-co-3HD) or poly(epsilon-caprolactone), was isolated and characterized by fatty acid analysis. Its cells contained mainly straight chain, saturated and unsaturated fatty acids, with a high portion of fatty acids with 16 carbons, and did not contain hydroxylated or branched fatty acids. Its 16 rDNA gene sequence was found to be related to those of members of Clostridium group I (Collins er al., 1994). In a simple in vitro test using strain LMG 16094 in pure culture, an extruded P(3HB-co-12% 3HV) film lost 10% of its initial mass within 21 days of anaerobic incubation.}},
  author       = {{Mergaert, Joris and Glorieux, Griet and Hauben, Lysiane and Storms, Virginie and Mau, Margit and Swings, Jean}},
  issn         = {{0723-2020}},
  journal      = {{SYSTEMATIC AND APPLIED MICROBIOLOGY}},
  keywords     = {{poly(3-hydroxyalkanoates),biodegradation,poly(3-hydroxybutyrate),poly(3-hydroxybutyrate-co-3-hydroxyvalerate),poly(3-hydroxyoctanoate-co-3-hydroxydecanoate),anaerobic sludge,Clostridium,ILYOBACTER-DELAFIELDII,DEGRADATION,POLYHYDROXYALKANOATES,TREES}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{407--413}},
  title        = {{Biodegradation of poly(3-hydroxyalkanoates) in anaerobic sludge and characterization of a poly(3-hydroxyalkanoates) degrading anaerobic bacterium}},
  url          = {{http://dx.doi.org/10.1016/S0723-2020(96)80070-1}},
  volume       = {{19}},
  year         = {{1996}},
}

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