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A semi-automated micronucleus-centromere assay to assess low-dose radiation exposure in human lymphocytes

Ans Baeyens, Rosemary Swanson, Olivia Herd, Elizabeth Ainsbury, Thulani Mabhengu, Pascale Willem, Hubert Thierens UGent, Jacobus P Slabbert and Anne Vral UGent (2011) INTERNATIONAL JOURNAL OF RADIATION BIOLOGY. 87(9). p.923-931
abstract
Purpose: The in vitro micronucleus (MN) assay is a reliable method to assess radiation-induced chromosomal damage in human peripheral blood lymphocytes. It is used to evaluate in vivo radiation over-exposure and to assess in vitro chromosomal radiosensitivity. A limitation of the MN assay is the relatively high and variable spontaneous MN frequency that restricts low-dose estimation to doses of about 0.3 gray (Gy). As radiation-induced MN mainly contain acentric fragments and spontaneous MN originate from lagging chromosomes, both MN types can be distinguished from each other by using fluorescence in situ hybridisation (FISH) with a pan-centromeric probe. The aim of this study was to investigate if the sensitivity, reliability and processing time of the MN assay can be enhanced by combining the automated MN assay with pan-centromere scoring. Materials and methods: Blood samples from 10 healthy donors were irradiated in vitro with low doses of gamma-rays. Dose response curves were determined for fully-automated and semi-automated MN scoring and semi-automated scoring of centromere negative MN (MNCM-). Results: A good correlation was obtained between fully-automated and semi-automated MN scoring (r<SU2</SU == 0.9973) and between fully automated MN scoring and semi-automated scoring of MNCM- (r<SU2</SU == 0.998). With the Wilcoxon test, a significant p value was obtained between 0 and 0.2 Gy for the fully-automated MN analysis, between 0 and 0.1 Gy for semi-automated MN analysis and between 0 and 0.05 Gy for semi-automated scoring of MNCM-. Conclusion: The semi-automated micronucleus-centromere assay combines high-speed MN analysis with a more accurate assessment in the low-dose range which makes it of special interest for large-scale radiation applications.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
PERIPHERAL-BLOOD LYMPHOCYTES, IN-SITU HYBRIDIZATION, CYTOKINESIS-BLOCKED LYMPHOCYTES, POWER-PLANT WORKERS, FREQUENCY, CHROMOSOMES, DAMAGE, BIOLOGICAL DOSIMETRY, RADIOLOGICAL WORKERS, IONIZING-RADIATION
journal title
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
Int. J. Radiat. Biol.
volume
87
issue
9
pages
923 - 931
Web of Science type
Article
Web of Science id
000294497900002
JCR category
NUCLEAR SCIENCE & TECHNOLOGY
JCR impact factor
2.275 (2011)
JCR rank
1/35 (2011)
JCR quartile
1 (2011)
ISSN
0955-3002
DOI
10.3109/09553002.2011.577508
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1908812
handle
http://hdl.handle.net/1854/LU-1908812
date created
2011-09-26 13:51:11
date last changed
2011-09-27 14:36:46
@article{1908812,
  abstract     = {Purpose: The in vitro micronucleus (MN) assay is a reliable method to assess radiation-induced chromosomal damage in human peripheral blood lymphocytes. It is used to evaluate in vivo radiation over-exposure and to assess in vitro chromosomal radiosensitivity. A limitation of the MN assay is the relatively high and variable spontaneous MN frequency that restricts low-dose estimation to doses of about 0.3 gray (Gy). As radiation-induced MN mainly contain acentric fragments and spontaneous MN originate from lagging chromosomes, both MN types can be distinguished from each other by using fluorescence in situ hybridisation (FISH) with a pan-centromeric probe. The aim of this study was to investigate if the sensitivity, reliability and processing time of the MN assay can be enhanced by combining the automated MN assay with pan-centromere scoring.
Materials and methods: Blood samples from 10 healthy donors were irradiated in vitro with low doses of gamma-rays. Dose response curves were determined for fully-automated and semi-automated MN scoring and semi-automated scoring of centromere negative MN (MNCM-).
Results: A good correlation was obtained between fully-automated and semi-automated MN scoring (r{\textlangle}SU2{\textlangle}/SU == 0.9973) and between fully automated MN scoring and semi-automated scoring of MNCM- (r{\textlangle}SU2{\textlangle}/SU == 0.998). With the Wilcoxon test, a significant p value was obtained between 0 and 0.2 Gy for the fully-automated MN analysis, between 0 and 0.1 Gy for semi-automated MN analysis and between 0 and 0.05 Gy for semi-automated scoring of MNCM-.
Conclusion: The semi-automated micronucleus-centromere assay combines high-speed MN analysis with a more accurate assessment in the low-dose range which makes it of special interest for large-scale radiation applications.},
  author       = {Baeyens, Ans and Swanson, Rosemary and Herd, Olivia and Ainsbury, Elizabeth and Mabhengu, Thulani and Willem, Pascale and Thierens, Hubert and Slabbert, Jacobus P and Vral, Anne},
  issn         = {0955-3002},
  journal      = {INTERNATIONAL JOURNAL OF RADIATION BIOLOGY},
  keyword      = {PERIPHERAL-BLOOD LYMPHOCYTES,IN-SITU HYBRIDIZATION,CYTOKINESIS-BLOCKED LYMPHOCYTES,POWER-PLANT WORKERS,FREQUENCY,CHROMOSOMES,DAMAGE,BIOLOGICAL DOSIMETRY,RADIOLOGICAL WORKERS,IONIZING-RADIATION},
  language     = {eng},
  number       = {9},
  pages        = {923--931},
  title        = {A semi-automated micronucleus-centromere assay to assess low-dose radiation exposure in human lymphocytes},
  url          = {http://dx.doi.org/10.3109/09553002.2011.577508},
  volume       = {87},
  year         = {2011},
}

Chicago
Baeyens, Ans, Rosemary Swanson, Olivia Herd, Elizabeth Ainsbury, Thulani Mabhengu, Pascale Willem, Hubert Thierens, Jacobus P Slabbert, and Anne Vral. 2011. “A Semi-automated Micronucleus-centromere Assay to Assess Low-dose Radiation Exposure in Human Lymphocytes.” International Journal of Radiation Biology 87 (9): 923–931.
APA
Baeyens, A., Swanson, R., Herd, O., Ainsbury, E., Mabhengu, T., Willem, P., Thierens, H., et al. (2011). A semi-automated micronucleus-centromere assay to assess low-dose radiation exposure in human lymphocytes. INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 87(9), 923–931.
Vancouver
1.
Baeyens A, Swanson R, Herd O, Ainsbury E, Mabhengu T, Willem P, et al. A semi-automated micronucleus-centromere assay to assess low-dose radiation exposure in human lymphocytes. INTERNATIONAL JOURNAL OF RADIATION BIOLOGY. 2011;87(9):923–31.
MLA
Baeyens, Ans, Rosemary Swanson, Olivia Herd, et al. “A Semi-automated Micronucleus-centromere Assay to Assess Low-dose Radiation Exposure in Human Lymphocytes.” INTERNATIONAL JOURNAL OF RADIATION BIOLOGY 87.9 (2011): 923–931. Print.