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Thin-layer chromatography of aflatoxins and zearalenones with beta-cyclodextrins as mobile phase additives

(2011) WORLD MYCOTOXIN JOURNAL. 4(2). p.113-117
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Abstract
The conditions of thin-layer chromatography separation of related aflatoxins and zearalenones in the presence of beta-cyclodextrin and 2-hydroxypropyl-beta-cyclodextrin were studied. Effects of the stationary phase and mobile phase composition were investigated. Analytical conditions for the separation and simultaneous semi-quantitative fluorescence detection of aflatoxins B(1), B(2), G(1) and G(2), zearalenone and alpha-zearalenol on normal-phase plates (silica gel, polyamide) and reversed-phase plates (C18) with cyclodextrin modified mobile phase were optimised. The limit of quantification was found 2 ng per spot for aflatoxin G(1) and aflatoxin B(2), 3.5 ng for aflatoxin B(1) and aflatoxin G(2) and 100 ng per spot for zearalenone and alpha-zearalenol. Addition of cyclodextrins to the mobile phase allowed a decrease in the amount of toxic solvents, and improved separation characteristics, but did not improve the limit of quantification.
Keywords
zearalenone, chromatography, aflatoxins, rapid methods, fluorescence, MYCOTOXINS, FLUORESCENCE, SEPARATION

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Citation

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Chicago
Larionova, Daria, IY Goryacheva, Carlos Van Peteghem, and Sarah De Saeger. 2011. “Thin-layer Chromatography of Aflatoxins and Zearalenones with Beta-cyclodextrins as Mobile Phase Additives.” World Mycotoxin Journal 4 (2): 113–117.
APA
Larionova, D., Goryacheva, I., Van Peteghem, C., & De Saeger, S. (2011). Thin-layer chromatography of aflatoxins and zearalenones with beta-cyclodextrins as mobile phase additives. WORLD MYCOTOXIN JOURNAL, 4(2), 113–117.
Vancouver
1.
Larionova D, Goryacheva I, Van Peteghem C, De Saeger S. Thin-layer chromatography of aflatoxins and zearalenones with beta-cyclodextrins as mobile phase additives. WORLD MYCOTOXIN JOURNAL. 2011;4(2):113–7.
MLA
Larionova, Daria, IY Goryacheva, Carlos Van Peteghem, et al. “Thin-layer Chromatography of Aflatoxins and Zearalenones with Beta-cyclodextrins as Mobile Phase Additives.” WORLD MYCOTOXIN JOURNAL 4.2 (2011): 113–117. Print.
@article{1908738,
  abstract     = {The conditions of thin-layer chromatography separation of related aflatoxins and zearalenones in the presence of beta-cyclodextrin and 2-hydroxypropyl-beta-cyclodextrin were studied. Effects of the stationary phase and mobile phase composition were investigated. Analytical conditions for the separation and simultaneous semi-quantitative fluorescence detection of aflatoxins B(1), B(2), G(1) and G(2), zearalenone and alpha-zearalenol on normal-phase plates (silica gel, polyamide) and reversed-phase plates (C18) with cyclodextrin modified mobile phase were optimised. The limit of quantification was found 2 ng per spot for aflatoxin G(1) and aflatoxin B(2), 3.5 ng for aflatoxin B(1) and aflatoxin G(2) and 100 ng per spot for zearalenone and alpha-zearalenol. Addition of cyclodextrins to the mobile phase allowed a decrease in the amount of toxic solvents, and improved separation characteristics, but did not improve the limit of quantification.},
  author       = {Larionova, Daria and Goryacheva, IY and Van Peteghem, Carlos and De Saeger, Sarah},
  issn         = {1875-0710},
  journal      = {WORLD MYCOTOXIN JOURNAL},
  keyword      = {zearalenone,chromatography,aflatoxins,rapid methods,fluorescence,MYCOTOXINS,FLUORESCENCE,SEPARATION},
  language     = {eng},
  number       = {2},
  pages        = {113--117},
  title        = {Thin-layer chromatography of aflatoxins and zearalenones with beta-cyclodextrins as mobile phase additives},
  url          = {http://dx.doi.org/10.3920/WMJ2010.1267},
  volume       = {4},
  year         = {2011},
}

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