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A validated multianalyte LC-MS/MS method for quantification of 25 mycotoxins in cassava flour, peanut cake and maize samples

Emmanuel Njumbe Ediage UGent, José Diana Di Mavungu UGent, Sofie Monbaliu UGent, Carlos Van Peteghem UGent and Sarah De Saeger UGent (2011) JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. 59(10). p.5173-5180
abstract
This study was designed to develop a sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the simultaneous detection and quantification of 25 mycotoxins in cassava flour, peanut cake and maize samples with particular focus on the optimization of the sample preparation protocol and method validation. All 25 mycotoxins were extracted in a single step with a mixture of methanol/ethyl acetate/water (70:20:10, v/v/v). The method limits of quantification (LOQ) varied from 0.3 mu g/kg to 106 mu g/kg. Good precision and linearity were observed for most of the mycotoxins. The method was applied for the analysis of naturally contaminated peanut cake, cassava flour and maize samples from the Republic of Benin. All samples analyzed (fifteen peanut cakes, four maize flour and four cassava flour samples) tested positive for one or more mycotoxins. Aflatoxins (total aflatoxins; 10-346 mu g/kg) and ochratoxin A (<LOQ-2 mu g/kg) were detected in peanut cake samples while fumonisin B(1) (4-21 mu g/kg), aflatoxin B(2) (<LOQ-8 mu g/kg), aflatoxin B(1) (<LOQ-9 mu g/kg), diacetoxyscirpenol (<LOQ-6 mu g/kg) and zearalenone (<LOQ-12 mu g/kg) were detected and quantified in cassava flour samples. Fumonisin B(1) (13-836 mu g/kg), fumonisin B(2) (5-221 mu g/kg), fumonisin B(3) (<LOQ-375 mu g/kg) and beauvericin (<LOQ-25 mu g/kg) were detected in the maize samples.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
WHEAT, GHANA, FOODS, BENIN, EXTRACTION, LIQUID, FUSARIUM MYCOTOXINS, AFLATOXIN CONTAMINATION, WEST-AFRICA, matrix effect, validation, maize, peanut cake, cassava, LC-MS/MS, multimycotoxin, CHROMATOGRAPHY/TANDEM MASS-SPECTROMETRY
journal title
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
J. Agric. Food Chem.
volume
59
issue
10
pages
5173 - 5180
Web of Science type
Article
Web of Science id
000290691300001
JCR category
AGRICULTURE, MULTIDISCIPLINARY
JCR impact factor
2.823 (2011)
JCR rank
3/57 (2011)
JCR quartile
1 (2011)
ISSN
0021-8561
DOI
10.1021/jf2009364
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1908721
handle
http://hdl.handle.net/1854/LU-1908721
date created
2011-09-26 11:15:17
date last changed
2011-09-27 13:48:24
@article{1908721,
  abstract     = {This study was designed to develop a sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the simultaneous detection and quantification of 25 mycotoxins in cassava flour, peanut cake and maize samples with particular focus on the optimization of the sample preparation protocol and method validation. All 25 mycotoxins were extracted in a single step with a mixture of methanol/ethyl acetate/water (70:20:10, v/v/v). The method limits of quantification (LOQ) varied from 0.3 mu g/kg to 106 mu g/kg. Good precision and linearity were observed for most of the mycotoxins. The method was applied for the analysis of naturally contaminated peanut cake, cassava flour and maize samples from the Republic of Benin. All samples analyzed (fifteen peanut cakes, four maize flour and four cassava flour samples) tested positive for one or more mycotoxins. Aflatoxins (total aflatoxins; 10-346 mu g/kg) and ochratoxin A ({\textlangle}LOQ-2 mu g/kg) were detected in peanut cake samples while fumonisin B(1) (4-21 mu g/kg), aflatoxin B(2) ({\textlangle}LOQ-8 mu g/kg), aflatoxin B(1) ({\textlangle}LOQ-9 mu g/kg), diacetoxyscirpenol ({\textlangle}LOQ-6 mu g/kg) and zearalenone ({\textlangle}LOQ-12 mu g/kg) were detected and quantified in cassava flour samples. Fumonisin B(1) (13-836 mu g/kg), fumonisin B(2) (5-221 mu g/kg), fumonisin B(3) ({\textlangle}LOQ-375 mu g/kg) and beauvericin ({\textlangle}LOQ-25 mu g/kg) were detected in the maize samples.},
  author       = {Njumbe Ediage, Emmanuel and Diana Di Mavungu, Jos{\'e} and Monbaliu, Sofie and Van Peteghem, Carlos and De Saeger, Sarah},
  issn         = {0021-8561},
  journal      = {JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY},
  keyword      = {WHEAT,GHANA,FOODS,BENIN,EXTRACTION,LIQUID,FUSARIUM MYCOTOXINS,AFLATOXIN CONTAMINATION,WEST-AFRICA,matrix effect,validation,maize,peanut cake,cassava,LC-MS/MS,multimycotoxin,CHROMATOGRAPHY/TANDEM MASS-SPECTROMETRY},
  language     = {eng},
  number       = {10},
  pages        = {5173--5180},
  title        = {A validated multianalyte LC-MS/MS method for quantification of 25 mycotoxins in cassava flour, peanut cake and maize samples},
  url          = {http://dx.doi.org/10.1021/jf2009364},
  volume       = {59},
  year         = {2011},
}

Chicago
Njumbe Ediage, Emmanuel, José Diana Di Mavungu, Sofie Monbaliu, Carlos Van Peteghem, and Sarah De Saeger. 2011. “A Validated Multianalyte LC-MS/MS Method for Quantification of 25 Mycotoxins in Cassava Flour, Peanut Cake and Maize Samples.” Journal of Agricultural and Food Chemistry 59 (10): 5173–5180.
APA
Njumbe Ediage, E., Diana Di Mavungu, J., Monbaliu, S., Van Peteghem, C., & De Saeger, S. (2011). A validated multianalyte LC-MS/MS method for quantification of 25 mycotoxins in cassava flour, peanut cake and maize samples. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 59(10), 5173–5180.
Vancouver
1.
Njumbe Ediage E, Diana Di Mavungu J, Monbaliu S, Van Peteghem C, De Saeger S. A validated multianalyte LC-MS/MS method for quantification of 25 mycotoxins in cassava flour, peanut cake and maize samples. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. 2011;59(10):5173–80.
MLA
Njumbe Ediage, Emmanuel, José Diana Di Mavungu, Sofie Monbaliu, et al. “A Validated Multianalyte LC-MS/MS Method for Quantification of 25 Mycotoxins in Cassava Flour, Peanut Cake and Maize Samples.” JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 59.10 (2011): 5173–5180. Print.