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A validated multianalyte LC-MS/MS method for quantification of 25 mycotoxins in cassava flour, peanut cake and maize samples

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Abstract
This study was designed to develop a sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the simultaneous detection and quantification of 25 mycotoxins in cassava flour, peanut cake and maize samples with particular focus on the optimization of the sample preparation protocol and method validation. All 25 mycotoxins were extracted in a single step with a mixture of methanol/ethyl acetate/water (70:20:10, v/v/v). The method limits of quantification (LOQ) varied from 0.3 mu g/kg to 106 mu g/kg. Good precision and linearity were observed for most of the mycotoxins. The method was applied for the analysis of naturally contaminated peanut cake, cassava flour and maize samples from the Republic of Benin. All samples analyzed (fifteen peanut cakes, four maize flour and four cassava flour samples) tested positive for one or more mycotoxins. Aflatoxins (total aflatoxins; 10-346 mu g/kg) and ochratoxin A (<LOQ-2 mu g/kg) were detected in peanut cake samples while fumonisin B(1) (4-21 mu g/kg), aflatoxin B(2) (<LOQ-8 mu g/kg), aflatoxin B(1) (<LOQ-9 mu g/kg), diacetoxyscirpenol (<LOQ-6 mu g/kg) and zearalenone (<LOQ-12 mu g/kg) were detected and quantified in cassava flour samples. Fumonisin B(1) (13-836 mu g/kg), fumonisin B(2) (5-221 mu g/kg), fumonisin B(3) (<LOQ-375 mu g/kg) and beauvericin (<LOQ-25 mu g/kg) were detected in the maize samples.
Keywords
WHEAT, GHANA, FOODS, BENIN, EXTRACTION, LIQUID, FUSARIUM MYCOTOXINS, AFLATOXIN CONTAMINATION, WEST-AFRICA, matrix effect, validation, maize, peanut cake, cassava, LC-MS/MS, multimycotoxin, CHROMATOGRAPHY/TANDEM MASS-SPECTROMETRY

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Citation

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Chicago
Njumbe Ediage, Emmanuel, José Diana Di Mavungu, Sofie Monbaliu, Carlos Van Peteghem, and Sarah De Saeger. 2011. “A Validated Multianalyte LC-MS/MS Method for Quantification of 25 Mycotoxins in Cassava Flour, Peanut Cake and Maize Samples.” Journal of Agricultural and Food Chemistry 59 (10): 5173–5180.
APA
Njumbe Ediage, E., Diana Di Mavungu, J., Monbaliu, S., Van Peteghem, C., & De Saeger, S. (2011). A validated multianalyte LC-MS/MS method for quantification of 25 mycotoxins in cassava flour, peanut cake and maize samples. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 59(10), 5173–5180.
Vancouver
1.
Njumbe Ediage E, Diana Di Mavungu J, Monbaliu S, Van Peteghem C, De Saeger S. A validated multianalyte LC-MS/MS method for quantification of 25 mycotoxins in cassava flour, peanut cake and maize samples. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. 2011;59(10):5173–80.
MLA
Njumbe Ediage, Emmanuel, José Diana Di Mavungu, Sofie Monbaliu, et al. “A Validated Multianalyte LC-MS/MS Method for Quantification of 25 Mycotoxins in Cassava Flour, Peanut Cake and Maize Samples.” JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 59.10 (2011): 5173–5180. Print.
@article{1908721,
  abstract     = {This study was designed to develop a sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the simultaneous detection and quantification of 25 mycotoxins in cassava flour, peanut cake and maize samples with particular focus on the optimization of the sample preparation protocol and method validation. All 25 mycotoxins were extracted in a single step with a mixture of methanol/ethyl acetate/water (70:20:10, v/v/v). The method limits of quantification (LOQ) varied from 0.3 mu g/kg to 106 mu g/kg. Good precision and linearity were observed for most of the mycotoxins. The method was applied for the analysis of naturally contaminated peanut cake, cassava flour and maize samples from the Republic of Benin. All samples analyzed (fifteen peanut cakes, four maize flour and four cassava flour samples) tested positive for one or more mycotoxins. Aflatoxins (total aflatoxins; 10-346 mu g/kg) and ochratoxin A ({\textlangle}LOQ-2 mu g/kg) were detected in peanut cake samples while fumonisin B(1) (4-21 mu g/kg), aflatoxin B(2) ({\textlangle}LOQ-8 mu g/kg), aflatoxin B(1) ({\textlangle}LOQ-9 mu g/kg), diacetoxyscirpenol ({\textlangle}LOQ-6 mu g/kg) and zearalenone ({\textlangle}LOQ-12 mu g/kg) were detected and quantified in cassava flour samples. Fumonisin B(1) (13-836 mu g/kg), fumonisin B(2) (5-221 mu g/kg), fumonisin B(3) ({\textlangle}LOQ-375 mu g/kg) and beauvericin ({\textlangle}LOQ-25 mu g/kg) were detected in the maize samples.},
  author       = {Njumbe Ediage, Emmanuel and Diana Di Mavungu, Jos{\'e} and Monbaliu, Sofie and Van Peteghem, Carlos and De Saeger, Sarah},
  issn         = {0021-8561},
  journal      = {JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY},
  keyword      = {WHEAT,GHANA,FOODS,BENIN,EXTRACTION,LIQUID,FUSARIUM MYCOTOXINS,AFLATOXIN CONTAMINATION,WEST-AFRICA,matrix effect,validation,maize,peanut cake,cassava,LC-MS/MS,multimycotoxin,CHROMATOGRAPHY/TANDEM MASS-SPECTROMETRY},
  language     = {eng},
  number       = {10},
  pages        = {5173--5180},
  title        = {A validated multianalyte LC-MS/MS method for quantification of 25 mycotoxins in cassava flour, peanut cake and maize samples},
  url          = {http://dx.doi.org/10.1021/jf2009364},
  volume       = {59},
  year         = {2011},
}

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