Sucrose-induced shrinkage of in vitro produced bovine morulae: effect on viability, morphology and ease of evaluation
- Author
- Ann Van Soom (UGent) , Maria Ysebaert, A Vanhoucke-De Medts, Aline Van de Velde, S Merton, A Delval, A Van Langendonckt, I Donnay, Geert Vanroose, Peter Bols and Aart de Kruif (UGent)
- Organization
- Abstract
- Sucrose (0.3 M) was used to cause artificial compaction of the embryonic cell mass of in vitro produced bovine embryos to facilitate morphological evaluation. Embryos were produced using routine in vitro maturation (IVM) and fertilization (IVF) techniques. The time necessary to induce shrinkage in 0.3 M sucrose to 75% of the original volume of Day 5 morulae was found to be less than 1 min, and 95% of the volume was regained in PBS after 2.5 min. No detrimental effect was observed after a 5- to 10-min sucrose treatment on subsequent blastocyst formation at Days 6 and 7 (P> 0.05). Furthermore, no significant differences were observed in the total number of cells, or in the mitotic and pycnotic cell index of blastocysts in different treatment groups. Agreement among 7 evaluators grading 40 Day 6 embryos was examined using the kappa coefficient of agreement (kappa). Overall agreement among evaluators for classification of quality grade was poor (48.2 %, kappa = 0.31) for embryos evaluated in PBS, but the rate improved when the same embryos were scored in sucrose (62.5 %, kappa = 0.49). Evaluating less compact in vitro produced bovine morulae in sucrose increases agreement among evaluators, since embryos in sucrose mimick the appearance of in vivo produced embryos. Thus, we conclude that scoring in vitro produced embryos in sucrose improves agreement among evaluators.
- Keywords
- sucrose, bovine morula compaction, embryo morphology, HYPERTONIC MEDIUM TREATMENT, IN-VITRO, EMBRYO MICROMANIPULATION, INVITRO, SURVIVAL, MOUSE, BLASTOCYST, OOCYTES, VIVO
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-190408
- MLA
- Van Soom, Ann, et al. “Sucrose-Induced Shrinkage of in Vitro Produced Bovine Morulae: Effect on Viability, Morphology and Ease of Evaluation.” THERIOGENOLOGY, vol. 46, no. 7, 1996, pp. 1131–47, doi:10.1016/S0093-691X(96)00285-3.
- APA
- Van Soom, A., Ysebaert, M., Vanhoucke-De Medts, A., Van de Velde, A., Merton, S., Delval, A., … de Kruif, A. (1996). Sucrose-induced shrinkage of in vitro produced bovine morulae: effect on viability, morphology and ease of evaluation. THERIOGENOLOGY, 46(7), 1131–1147. https://doi.org/10.1016/S0093-691X(96)00285-3
- Chicago author-date
- Van Soom, Ann, Maria Ysebaert, A Vanhoucke-De Medts, Aline Van de Velde, S Merton, A Delval, A Van Langendonckt, et al. 1996. “Sucrose-Induced Shrinkage of in Vitro Produced Bovine Morulae: Effect on Viability, Morphology and Ease of Evaluation.” THERIOGENOLOGY 46 (7): 1131–47. https://doi.org/10.1016/S0093-691X(96)00285-3.
- Chicago author-date (all authors)
- Van Soom, Ann, Maria Ysebaert, A Vanhoucke-De Medts, Aline Van de Velde, S Merton, A Delval, A Van Langendonckt, I Donnay, Geert Vanroose, Peter Bols, and Aart de Kruif. 1996. “Sucrose-Induced Shrinkage of in Vitro Produced Bovine Morulae: Effect on Viability, Morphology and Ease of Evaluation.” THERIOGENOLOGY 46 (7): 1131–1147. doi:10.1016/S0093-691X(96)00285-3.
- Vancouver
- 1.Van Soom A, Ysebaert M, Vanhoucke-De Medts A, Van de Velde A, Merton S, Delval A, et al. Sucrose-induced shrinkage of in vitro produced bovine morulae: effect on viability, morphology and ease of evaluation. THERIOGENOLOGY. 1996;46(7):1131–47.
- IEEE
- [1]A. Van Soom et al., “Sucrose-induced shrinkage of in vitro produced bovine morulae: effect on viability, morphology and ease of evaluation,” THERIOGENOLOGY, vol. 46, no. 7, pp. 1131–1147, 1996.
@article{190408,
abstract = {{Sucrose (0.3 M) was used to cause artificial compaction of the embryonic cell mass of in vitro produced bovine embryos to facilitate morphological evaluation. Embryos were produced using routine in vitro maturation (IVM) and fertilization (IVF) techniques. The time necessary to induce shrinkage in 0.3 M sucrose to 75% of the original volume of Day 5 morulae was found to be less than 1 min, and 95% of the volume was regained in PBS after 2.5 min. No detrimental effect was observed after a 5- to 10-min sucrose treatment on subsequent blastocyst formation at Days 6 and 7 (P> 0.05). Furthermore, no significant differences were observed in the total number of cells, or in the mitotic and pycnotic cell index of blastocysts in different treatment groups. Agreement among 7 evaluators grading 40 Day 6 embryos was examined using the kappa coefficient of agreement (kappa). Overall agreement among evaluators for classification of quality grade was poor (48.2 %, kappa = 0.31) for embryos evaluated in PBS, but the rate improved when the same embryos were scored in sucrose (62.5 %, kappa = 0.49). Evaluating less compact in vitro produced bovine morulae in sucrose increases agreement among evaluators, since embryos in sucrose mimick the appearance of in vivo produced embryos. Thus, we conclude that scoring in vitro produced embryos in sucrose improves agreement among evaluators.}},
author = {{Van Soom, Ann and Ysebaert, Maria and Vanhoucke-De Medts, A and Van de Velde, Aline and Merton, S and Delval, A and Van Langendonckt, A and Donnay, I and Vanroose, Geert and Bols, Peter and de Kruif, Aart}},
issn = {{0093-691X}},
journal = {{THERIOGENOLOGY}},
keywords = {{sucrose,bovine morula compaction,embryo morphology,HYPERTONIC MEDIUM TREATMENT,IN-VITRO,EMBRYO MICROMANIPULATION,INVITRO,SURVIVAL,MOUSE,BLASTOCYST,OOCYTES,VIVO}},
language = {{eng}},
number = {{7}},
pages = {{1131--1147}},
title = {{Sucrose-induced shrinkage of in vitro produced bovine morulae: effect on viability, morphology and ease of evaluation}},
url = {{http://doi.org/10.1016/S0093-691X(96)00285-3}},
volume = {{46}},
year = {{1996}},
}
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