Advanced search
1 file | 1.14 MB

Identification and purification of a bacterial ice-nucleation protein

Author
Organization
Abstract
The protein product of a gene (inaZ) responsible for ice nucleation by Pseudomonas syringae S203 has been identified and purified after overexpression in Escherichia coli. The amino acid composition and the N-terminal sequence of the purified, denatured protein corresponded well with that predicted from the sequence of the inaZ gene. The product of inaZ was also found to be the major component in preparations of ice-nucleating, proteinaceous particles, obtained after extraction with and gel filtration in a mixture of urea and the nondenaturing detergent octyl beta-D-thioglucopyranoside. The activity of these preparations in the absence of added lipid implies that the protein participates directly in the nucleation process.

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 1.14 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Wolber, Paul K, Caroline A Deininger, Maurice W Southworth, Joël Vandekerckhove, Marc Van Montagu, and Gareth J Warren. 1986. “Identification and Purification of a Bacterial Ice-nucleation Protein.” Proceedings of the National Academy of Sciences of the United States of America 83 (19): 7256–7260.
APA
Wolber, P. K., Deininger, C. A., Southworth, M. W., Vandekerckhove, J., Van Montagu, M., & Warren, G. J. (1986). Identification and purification of a bacterial ice-nucleation protein. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 83(19), 7256–7260.
Vancouver
1.
Wolber PK, Deininger CA, Southworth MW, Vandekerckhove J, Van Montagu M, Warren GJ. Identification and purification of a bacterial ice-nucleation protein. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 1986;83(19):7256–60.
MLA
Wolber, Paul K, Caroline A Deininger, Maurice W Southworth, et al. “Identification and Purification of a Bacterial Ice-nucleation Protein.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 83.19 (1986): 7256–7260. Print.
@article{1903654,
  abstract     = {The protein product of a gene (inaZ) responsible for ice nucleation by Pseudomonas syringae S203 has been identified and purified after overexpression in Escherichia coli. The amino acid composition and the N-terminal sequence of the purified, denatured protein corresponded well with that predicted from the sequence of the inaZ gene. The product of inaZ was also found to be the major component in preparations of ice-nucleating, proteinaceous particles, obtained after extraction with and gel filtration in a mixture of urea and the nondenaturing detergent octyl beta-D-thioglucopyranoside. The activity of these preparations in the absence of added lipid implies that the protein participates directly in the nucleation process.},
  author       = {Wolber, Paul K and Deininger, Caroline A and Southworth, Maurice W and Vandekerckhove, Jo{\"e}l and Van Montagu, Marc and Warren, Gareth J},
  issn         = {0027-8424},
  journal      = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA},
  language     = {eng},
  number       = {19},
  pages        = {7256--7260},
  title        = {Identification and purification of a bacterial ice-nucleation protein},
  url          = {http://dx.doi.org/10.1073/pnas.83.19.7256},
  volume       = {83},
  year         = {1986},
}

Altmetric
View in Altmetric