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Identification of plant promoters in situ by T-DNA-mediated transcriptional fusions to the npt-II gene

(1986) EMBO JOURNAL. 5(8). p.1755-1760
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Abstract
We have constructed an Agrobacterium-mediated plant vector,pHTT88,which upon integration into the plant chromosomes can produce transcriptional fusions between upstream control elements in the plant genome and the gene for neomycin phosphotransferase II (npt-II). The vector is based on a juxtaposition of a T-DNA right border to the coding sequence of npt-II. Using this system it is possible to isolate kanamycin-resistant plant calli where the site of integration is suitably positioned downstream of plant transcription signals. We have demonstrated that the NPT-II activity of such tobacco transformants shows different tissue-specific patterns of regulation in the regenerated plants. Among eight transformants analyzed, we have isolated one gene fusion expressed only in roots and others expressed only in stem. The results demonstrate that a gene fusion vector can be used effectively in plants to identify and characterize regulatory elements.

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Citation

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MLA
Teeri, TH, Luis Herrera-Estrella, Anna Depicker, et al. “Identification of Plant Promoters in Situ by T-DNA-mediated Transcriptional Fusions to the npt-II Gene.” EMBO JOURNAL 5.8 (1986): 1755–1760. Print.
APA
Teeri, T., Herrera-Estrella, L., Depicker, A., Van Montagu, M., & Palva, E. (1986). Identification of plant promoters in situ by T-DNA-mediated transcriptional fusions to the npt-II gene. EMBO JOURNAL, 5(8), 1755–1760.
Chicago author-date
Teeri, TH, Luis Herrera-Estrella, Anna Depicker, Marc Van Montagu, and ET Palva. 1986. “Identification of Plant Promoters in Situ by T-DNA-mediated Transcriptional Fusions to the npt-II Gene.” Embo Journal 5 (8): 1755–1760.
Chicago author-date (all authors)
Teeri, TH, Luis Herrera-Estrella, Anna Depicker, Marc Van Montagu, and ET Palva. 1986. “Identification of Plant Promoters in Situ by T-DNA-mediated Transcriptional Fusions to the npt-II Gene.” Embo Journal 5 (8): 1755–1760.
Vancouver
1.
Teeri T, Herrera-Estrella L, Depicker A, Van Montagu M, Palva E. Identification of plant promoters in situ by T-DNA-mediated transcriptional fusions to the npt-II gene. EMBO JOURNAL. 1986;5(8):1755–60.
IEEE
[1]
T. Teeri, L. Herrera-Estrella, A. Depicker, M. Van Montagu, and E. Palva, “Identification of plant promoters in situ by T-DNA-mediated transcriptional fusions to the npt-II gene,” EMBO JOURNAL, vol. 5, no. 8, pp. 1755–1760, 1986.
@article{1903610,
  abstract     = {We have constructed an Agrobacterium-mediated plant vector,pHTT88,which upon integration into the plant chromosomes can produce transcriptional fusions between upstream control elements in the plant genome and the gene for neomycin phosphotransferase II (npt-II). The vector is based on a juxtaposition of a T-DNA right border to the coding sequence of npt-II. Using this system it is possible to isolate kanamycin-resistant plant calli where the site of integration is suitably positioned downstream of plant transcription signals. We have demonstrated that the NPT-II activity of such tobacco transformants shows different tissue-specific patterns of regulation in the regenerated plants. Among eight transformants analyzed, we have isolated one gene fusion expressed only in roots and others expressed only in stem. The results demonstrate that a gene fusion vector can be used effectively in plants to identify and characterize regulatory elements.},
  author       = {Teeri, TH and Herrera-Estrella, Luis and Depicker, Anna and Van Montagu, Marc and Palva, ET},
  issn         = {0261-4189},
  journal      = {EMBO JOURNAL},
  language     = {eng},
  number       = {8},
  pages        = {1755--1760},
  title        = {Identification of plant promoters in situ by T-DNA-mediated transcriptional fusions to the npt-II gene},
  url          = {http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1167037/?tool=pubmed},
  volume       = {5},
  year         = {1986},
}