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Structural and functional analysis of a cloned delta endotoxin of Bacillus thuringiensis berliner 1715

Herman Höfte, Henri De Greve, Jef Seurinck, Stefan Jansen, Jacques Mahillon, Christophe Ampe UGent, Joël Vandekerckhove UGent, Hilde Vanderbruggen, Marc Van Montagu UGent and Marc Zabeau UGent, et al. (1986) EUROPEAN JOURNAL OF BIOCHEMISTRY. 161(2). p.273-280
abstract
A plasmid-encoded crystal protein gene (bt2) has been cloned from Bacillus thuringiensis berliner 1715. In Escherichia coli, it directs the synthesis of the 130-kDa protein (Bt2) which is toxic to larvae of Pieris brassicae and Manduca sexta. Comparison of the deduced amino acid sequence of this Bt2 protein with the B. thuringiensis kurstaki HD1 Dipel, B. thuringiensis kurstaki HD73 and B. thuringiensis sotto crystal protein sequences suggests that homologous recombination between the different genes has occurred during evolution. Treatment of the Bt2 protein with trypsin or chymotrypsin yields a 60-kDa protease-resistant and fully toxic polypeptide. The minimal portion of the Bt2 protein required for toxicity has been determined by analysing the polypeptides produced by deletion derivatives of the bt2 gene. It coincides with the 60-kDa protease-resistant Bt2 fragment and it starts between amino acids 29 and 35 at the N-terminus and terminates between positions 599 and 607 at the C-terminus.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
journal title
EUROPEAN JOURNAL OF BIOCHEMISTRY
Eur. J. Biochem.
volume
161
issue
2
pages
273 - 280
Web of Science type
Article
ISSN
0014-2956
DOI
10.1111/j.1432-1033.1986.tb10443.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1903400
handle
http://hdl.handle.net/1854/LU-1903400
date created
2011-09-19 11:27:57
date last changed
2011-09-21 11:46:22
@article{1903400,
  abstract     = {A plasmid-encoded crystal protein gene (bt2) has been cloned from Bacillus thuringiensis berliner 1715. In Escherichia coli, it directs the synthesis of the 130-kDa protein (Bt2) which is toxic to larvae of Pieris brassicae and Manduca sexta. Comparison of the deduced amino acid sequence of this Bt2 protein with the B. thuringiensis kurstaki HD1 Dipel, B. thuringiensis kurstaki HD73 and B. thuringiensis sotto crystal protein sequences suggests that homologous recombination between the different genes has occurred during evolution. Treatment of the Bt2 protein with trypsin or chymotrypsin yields a 60-kDa protease-resistant and fully toxic polypeptide. The minimal portion of the Bt2 protein required for toxicity has been determined by analysing the polypeptides produced by deletion derivatives of the bt2 gene. It coincides with the 60-kDa protease-resistant Bt2 fragment and it starts between amino acids 29 and 35 at the N-terminus and terminates between positions 599 and 607 at the C-terminus.},
  author       = {H{\"o}fte, Herman and De Greve, Henri and Seurinck, Jef and Jansen, Stefan and Mahillon, Jacques and Ampe, Christophe and Vandekerckhove, Jo{\"e}l and Vanderbruggen, Hilde and Van Montagu, Marc and Zabeau, Marc and Vaeck, Mark},
  issn         = {0014-2956},
  journal      = {EUROPEAN JOURNAL OF BIOCHEMISTRY},
  language     = {eng},
  number       = {2},
  pages        = {273--280},
  title        = {Structural and functional analysis of a cloned delta endotoxin of Bacillus thuringiensis berliner 1715},
  url          = {http://dx.doi.org/10.1111/j.1432-1033.1986.tb10443.x},
  volume       = {161},
  year         = {1986},
}

Chicago
Höfte, Herman, Henri De Greve, Jef Seurinck, Stefan Jansen, Jacques Mahillon, Christophe Ampe, Joël Vandekerckhove, et al. 1986. “Structural and Functional Analysis of a Cloned Delta Endotoxin of Bacillus Thuringiensis Berliner 1715.” European Journal of Biochemistry 161 (2): 273–280.
APA
Höfte, H., De Greve, H., Seurinck, J., Jansen, S., Mahillon, J., Ampe, C., Vandekerckhove, J., et al. (1986). Structural and functional analysis of a cloned delta endotoxin of Bacillus thuringiensis berliner 1715. EUROPEAN JOURNAL OF BIOCHEMISTRY, 161(2), 273–280.
Vancouver
1.
Höfte H, De Greve H, Seurinck J, Jansen S, Mahillon J, Ampe C, et al. Structural and functional analysis of a cloned delta endotoxin of Bacillus thuringiensis berliner 1715. EUROPEAN JOURNAL OF BIOCHEMISTRY. 1986;161(2):273–80.
MLA
Höfte, Herman, Henri De Greve, Jef Seurinck, et al. “Structural and Functional Analysis of a Cloned Delta Endotoxin of Bacillus Thuringiensis Berliner 1715.” EUROPEAN JOURNAL OF BIOCHEMISTRY 161.2 (1986): 273–280. Print.