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Promoters of Agrobacterium tumefaciens Ti-plasmid virulence genes

A Das, Scott Stachel, P Ebert, P Allenza, A Montoya and E Nester (1986) NUCLEIC ACIDS RESEARCH. 14(3). p.1355-1364
abstract
The DNA sequences of the promoter and 5' upstream regions of six Agrobacterium tumefaciens Ti-plasmid encoded virulence (vir) genes were determined. The transcription initiation sites were mapped by the S1 nuclease protection assay. In the -10 region, the vir promoters share a consensus sequence that is homologous to a DNA sequence found in the same region of E. coli promoters. In contrast, the -35 region sequences are variable. Several vir genes contain two common hexanucleotide sequences, 5'CGAGTA3' and 5'GCAATT3'. Translation initiation codons for all vir genes, except virG, are preceded by sequences homologous to the ribosome binding site sequences found in E. coli.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
journal title
NUCLEIC ACIDS RESEARCH
Nucleic Acids Res.
volume
14
issue
3
pages
1355 - 1364
Web of Science type
Article
ISSN
0305-1048
DOI
10.1093/nar/14.3.1355
language
English
UGent publication?
no
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1903322
handle
http://hdl.handle.net/1854/LU-1903322
date created
2011-09-19 11:04:15
date last changed
2018-06-21 07:22:07
@article{1903322,
  abstract     = {The DNA sequences of the promoter and 5' upstream regions of six Agrobacterium tumefaciens Ti-plasmid encoded virulence (vir) genes were determined. The transcription initiation sites were mapped by the S1 nuclease protection assay. In the -10 region, the vir promoters share a consensus sequence that is homologous to a DNA sequence found in the same region of E. coli promoters. In contrast, the -35 region sequences are variable. Several vir genes contain two common hexanucleotide sequences, 5'CGAGTA3' and 5'GCAATT3'. Translation initiation codons for all vir genes, except virG, are preceded by sequences homologous to the ribosome binding site sequences found in E. coli.},
  author       = {Das, A and Stachel, Scott and Ebert, P and Allenza, P and Montoya, A and Nester, E},
  issn         = {0305-1048},
  journal      = {NUCLEIC ACIDS RESEARCH},
  language     = {eng},
  number       = {3},
  pages        = {1355--1364},
  title        = {Promoters of Agrobacterium tumefaciens Ti-plasmid virulence genes},
  url          = {http://dx.doi.org/10.1093/nar/14.3.1355},
  volume       = {14},
  year         = {1986},
}

Chicago
Das, A, Scott Stachel, P Ebert, P Allenza, A Montoya, and E Nester. 1986. “Promoters of Agrobacterium Tumefaciens Ti-plasmid Virulence Genes.” Nucleic Acids Research 14 (3): 1355–1364.
APA
Das, A, Stachel, S., Ebert, P., Allenza, P., Montoya, A., & Nester, E. (1986). Promoters of Agrobacterium tumefaciens Ti-plasmid virulence genes. NUCLEIC ACIDS RESEARCH, 14(3), 1355–1364.
Vancouver
1.
Das A, Stachel S, Ebert P, Allenza P, Montoya A, Nester E. Promoters of Agrobacterium tumefaciens Ti-plasmid virulence genes. NUCLEIC ACIDS RESEARCH. 1986;14(3):1355–64.
MLA
Das, A, Scott Stachel, P Ebert, et al. “Promoters of Agrobacterium Tumefaciens Ti-plasmid Virulence Genes.” NUCLEIC ACIDS RESEARCH 14.3 (1986): 1355–1364. Print.