Ghent University Academic Bibliography

Advanced

Activation of Agrobacterium tumefaciens vir gene expression generates multiple single-stranded T-strand molecules from the pTiA6 T-region: requirement for 5' virD gene products

Scott E Stachel, Benedikt Timmerman and Patricia Zambryski (1987) EMBO JOURNAL. 6(4). p.857-863
abstract
Agrobacterium tumefaciens transfers its Ti-plasmid T-DNA to plant cells. This process is initiated by plant-induced activation of the Ti-plasmid virulence loci, resulting in the generation of single stranded (ss) cleavages of the Ti-plasmid T-DNA border sequences (border nicks) and ss linear unipolar T-DNA molecules (T-strands). A single T-strand is produced from the two-border T-region of the pGV3850 nopaline plasmid. In this paper the induced molecular events for the complex T-region of the pTiA6 octopine plasmid are analyzed. This T-region carries four T-DNA borders delimiting three T-DNA elements (TR, TC and TL). Induction of pTiA6 generates cleavages independently at its border repeats, and six distinct T-strand species corresponding to TR, TR/TC, TR/TC/TL, TC, TC/TL and TL. These T-strand molecules are linear and correspond to the bottom strand of the pTiA6 T-region. Thus, borders can function for both initiation and termination of T-strand synthesis. We propose that the different pTiA6 T-strands are independently generated, and that the distribution of border nicks within the parental T-region determines which T-strand is produced. To identify genes involved in T-strand production, pTiA6 virulence (vir) and chromosomal virulence (chv) mutant strains were analyzed. VirA and VirG, the vir regulatory loci are required. Furthermore, the two 5' cistrons of virD are required for both border nicks and T-strands, suggesting that these genes encode the border endonuclease, and that T-strand production is dependent on border nicks. That no mutants are defective for T-strands alone suggests that functions encoded outside of vir and chv might mediate some of the later reactions of T-strand synthesis.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
journal title
EMBO JOURNAL
Embo J.
volume
6
issue
4
pages
857 - 863
Web of Science type
Article
ISSN
0261-4189
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1903056
handle
http://hdl.handle.net/1854/LU-1903056
alternative location
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC553475/?tool=pubmed
date created
2011-09-19 09:15:15
date last changed
2011-09-19 16:47:27
@article{1903056,
  abstract     = {Agrobacterium tumefaciens transfers its Ti-plasmid T-DNA to plant cells. This process is initiated by plant-induced activation of the Ti-plasmid virulence loci, resulting in the generation of single stranded (ss) cleavages of the Ti-plasmid T-DNA border sequences (border nicks) and ss linear unipolar T-DNA molecules (T-strands). A single T-strand is produced from the two-border T-region of the pGV3850 nopaline plasmid. In this paper the induced molecular events for the complex T-region of the pTiA6 octopine plasmid are analyzed. This T-region carries four T-DNA borders delimiting three T-DNA elements (TR, TC and TL). Induction of pTiA6 generates cleavages independently at its border repeats, and six distinct T-strand species corresponding to TR, TR/TC, TR/TC/TL, TC, TC/TL and TL. These T-strand molecules are linear and correspond to the bottom strand of the pTiA6 T-region. Thus, borders can function for both initiation and termination of T-strand synthesis. We propose that the different pTiA6 T-strands are independently generated, and that the distribution of border nicks within the parental T-region determines which T-strand is produced. To identify genes involved in T-strand production, pTiA6 virulence (vir) and chromosomal virulence (chv) mutant strains were analyzed. VirA and VirG, the vir regulatory loci are required. Furthermore, the two 5' cistrons of virD are required for both border nicks and T-strands, suggesting that these genes encode the border endonuclease, and that T-strand production is dependent on border nicks. That no mutants are defective for T-strands alone suggests that functions encoded outside of vir and chv might mediate some of the later reactions of T-strand synthesis.},
  author       = {Stachel, Scott E and Timmerman, Benedikt and Zambryski, Patricia},
  issn         = {0261-4189},
  journal      = {EMBO JOURNAL},
  language     = {eng},
  number       = {4},
  pages        = {857--863},
  title        = {Activation of Agrobacterium tumefaciens vir gene expression generates multiple single-stranded T-strand molecules from the pTiA6 T-region: requirement for 5' virD gene products},
  url          = {http://www.ncbi.nlm.nih.gov/pmc/articles/PMC553475/?tool=pubmed},
  volume       = {6},
  year         = {1987},
}

Chicago
Stachel, Scott E, Benedikt Timmerman, and Patricia Zambryski. 1987. “Activation of Agrobacterium Tumefaciens Vir Gene Expression Generates Multiple Single-stranded T-strand Molecules from the pTiA6 T-region: Requirement for 5’ virD Gene Products.” Embo Journal 6 (4): 857–863.
APA
Stachel, S. E., Timmerman, B., & Zambryski, P. (1987). Activation of Agrobacterium tumefaciens vir gene expression generates multiple single-stranded T-strand molecules from the pTiA6 T-region: requirement for 5’ virD gene products. EMBO JOURNAL, 6(4), 857–863.
Vancouver
1.
Stachel SE, Timmerman B, Zambryski P. Activation of Agrobacterium tumefaciens vir gene expression generates multiple single-stranded T-strand molecules from the pTiA6 T-region: requirement for 5’ virD gene products. EMBO JOURNAL. 1987;6(4):857–63.
MLA
Stachel, Scott E, Benedikt Timmerman, and Patricia Zambryski. “Activation of Agrobacterium Tumefaciens Vir Gene Expression Generates Multiple Single-stranded T-strand Molecules from the pTiA6 T-region: Requirement for 5’ virD Gene Products.” EMBO JOURNAL 6.4 (1987): 857–863. Print.