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T-DNA organization in tumor cultures and transgenic plants of the monocotyledon Asparagus officinalis

Benny Bytebier, Francine Deboeck, Henri De Greve, Marc Van Montagu UGent and Jean-Pierre Hernalsteens (1987) PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 84(15). p.5345-5349
abstract
Asparagus officinalis was the first monocotyledonous plant from which hormone-independent and opine-producing crown gall tissue could be isolated. We confirm by DNA hybridization that tumor lines obtained after infection of this plant by Agrobacterium strains harboring wild-type nopaline and octopine tumor-inducing (Ti) plasmids are stably transformed and contain transferred DNA (T-DNA) segments identical to the T-DNA found in dicotyledonous plants. We have also infected Asparagus with a nononcogenic T-DNA vector that carries a chimeric aminoglycoside phosphotransferase [NOS-APH(3')II] gene and selected transformed tissues on kanamycin-containing medium. The transformed status of these tissues was then confirmed by DNA hybridization. From these calli we regenerated kanamycin-resistant shoots that were subsequently rooted. Thus we report the isolation of transgenic monocotyledonous plants engineered via the Agrobacterium vector system.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
journal title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Proc. Natl. Acad. Sci. USA
volume
84
issue
15
pages
5345 - 5349
Web of Science type
Article
ISSN
0027-8424
DOI
10.1073/pnas.84.15.5345
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1902306
handle
http://hdl.handle.net/1854/LU-1902306
date created
2011-09-16 15:45:18
date last changed
2011-09-20 09:18:47
@article{1902306,
  abstract     = {Asparagus officinalis was the first monocotyledonous plant from which hormone-independent and opine-producing crown gall tissue could be isolated. We confirm by DNA hybridization that tumor lines obtained after infection of this plant by Agrobacterium strains harboring wild-type nopaline and octopine tumor-inducing (Ti) plasmids are stably transformed and contain transferred DNA (T-DNA) segments identical to the T-DNA found in dicotyledonous plants. We have also infected Asparagus with a nononcogenic T-DNA vector that carries a chimeric aminoglycoside phosphotransferase [NOS-APH(3')II] gene and selected transformed tissues on kanamycin-containing medium. The transformed status of these tissues was then confirmed by DNA hybridization. From these calli we regenerated kanamycin-resistant shoots that were subsequently rooted. Thus we report the isolation of transgenic monocotyledonous plants engineered via the Agrobacterium vector system.},
  author       = {Bytebier, Benny and Deboeck, Francine and De Greve, Henri and Van Montagu, Marc and Hernalsteens, Jean-Pierre},
  issn         = {0027-8424},
  journal      = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA},
  language     = {eng},
  number       = {15},
  pages        = {5345--5349},
  title        = {T-DNA organization in tumor cultures and transgenic plants of the monocotyledon Asparagus officinalis},
  url          = {http://dx.doi.org/10.1073/pnas.84.15.5345},
  volume       = {84},
  year         = {1987},
}

Chicago
Bytebier, Benny, Francine Deboeck, Henri De Greve, Marc Van Montagu, and Jean-Pierre Hernalsteens. 1987. “T-DNA Organization in Tumor Cultures and Transgenic Plants of the Monocotyledon Asparagus Officinalis.” Proceedings of the National Academy of Sciences of the United States of America 84 (15): 5345–5349.
APA
Bytebier, B., Deboeck, F., De Greve, H., Van Montagu, M., & Hernalsteens, J.-P. (1987). T-DNA organization in tumor cultures and transgenic plants of the monocotyledon Asparagus officinalis. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 84(15), 5345–5349.
Vancouver
1.
Bytebier B, Deboeck F, De Greve H, Van Montagu M, Hernalsteens J-P. T-DNA organization in tumor cultures and transgenic plants of the monocotyledon Asparagus officinalis. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 1987;84(15):5345–9.
MLA
Bytebier, Benny, Francine Deboeck, Henri De Greve, et al. “T-DNA Organization in Tumor Cultures and Transgenic Plants of the Monocotyledon Asparagus Officinalis.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 84.15 (1987): 5345–5349. Print.