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Impact of a perfluorinated organic compound PFOS on the terrestrial pollinator Bombus terrestris (Insecta, Hymenoptera)

Veerle Mommaerts UGent, An Hagenaars, Johan Meyer, Wim De Coen, Luc Swevers, Hadi Mosallanejad UGent and Guy Smagghe UGent (2011) ECOTOXICOLOGY. 20(2). p.447-456
abstract
Perfluorinated organic chemicals like perfluorooctane sulfonic acid (PFOS) are persistent environmental pollutants that have been measured in a great diversity of wildlife worldwide, especially in the aquatic compartment. However, little information is available on the presence and effects of PFOS in the terrestrial compartment. Therefore, we investigated in this project the risks for effects, bioaccumulation and potential mechanisms of activity of PFOS in the bumblebee Bombus terrestris L. (Hymenoptera: Apidae) that is an important worldwide pollinator in the terrestrial compartment of wildflowers and cultivated crops. The exposure to PFOS occurred orally via the drinking of treated sugar water in a wide range from 1 mu g/l up to 10 mg/l, containing environmentally relevant as well as high concentrations, and this was done with use of microcolonies of B. terrestris in the laboratory. A chronic toxicity assay demonstrated high bumblebee worker mortality (up to 100%) with an LC(50) of 1.01 mg/l (R (2) = 0.98). In addition, PFOS posed strong detrimental reproductive effects, and these concerted with a dramatic reduction in ovarian size. HPLC-MS demonstrated a bioaccumulation factor of 27.9 for PFOS in bumblebee workers fed with sugar water containing 100 mu g/l PFOS during 5 weeks (2184 +/- A 365 ng/g BW). Finally, potential mechanisms of activity were investigated to explain the significant impact of PFOS on survival and reproduction capacity of B. terrestris. Exposure of bumblebee workers to PFOS resulted in a significant decrease in mitochondrial electron transport activity (p = 0.035) and lipid amounts (p = 0.019), while the respective p-values were 0.58 and 0.12 for protein and glucose amounts. Hence, addition of PFOS to ecdysteroid responsive Drosophila melanogaster S2 cells resulted in a strong antagonistic action on the EcR-b.act.luc reporter construct, demonstrating that PFOS may exert its effects partially through an endocrine disrupting action via the insect molting hormone or ecdysteroid receptor.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Bumblebee, PFOS, Survival, Reproduction, Electron transport, Energy content, Antagonistic activity, PERFLUOROOCTANE SULFONIC-ACID, DEVELOPMENTAL TOXICITY, GENE-EXPRESSION, ENALLAGMA-CYATHIGERUM, PERFLUOROALKYL ACIDS, DAPHNIA-MAGNA, LIFE-CYCLE, CELL-LINES, IN-VITRO, EXPOSURE
journal title
ECOTOXICOLOGY
Ecotoxicology
volume
20
issue
2
pages
447 - 456
Web of Science type
Article
Web of Science id
000287245000015
JCR category
ENVIRONMENTAL SCIENCES
JCR impact factor
2.355 (2011)
JCR rank
60/203 (2011)
JCR quartile
2 (2011)
ISSN
0963-9292
DOI
10.1007/s10646-011-0596-2
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1901326
handle
http://hdl.handle.net/1854/LU-1901326
date created
2011-09-14 15:43:58
date last changed
2016-12-19 15:41:56
@article{1901326,
  abstract     = {Perfluorinated organic chemicals like perfluorooctane sulfonic acid (PFOS) are persistent environmental pollutants that have been measured in a great diversity of wildlife worldwide, especially in the aquatic compartment. However, little information is available on the presence and effects of PFOS in the terrestrial compartment. Therefore, we investigated in this project the risks for effects, bioaccumulation and potential mechanisms of activity of PFOS in the bumblebee Bombus terrestris L. (Hymenoptera: Apidae) that is an important worldwide pollinator in the terrestrial compartment of wildflowers and cultivated crops. The exposure to PFOS occurred orally via the drinking of treated sugar water in a wide range from 1 mu g/l up to 10 mg/l, containing environmentally relevant as well as high concentrations, and this was done with use of microcolonies of B. terrestris in the laboratory. A chronic toxicity assay demonstrated high bumblebee worker mortality (up to 100\%) with an LC(50) of 1.01 mg/l (R (2) = 0.98). In addition, PFOS posed strong detrimental reproductive effects, and these concerted with a dramatic reduction in ovarian size. HPLC-MS demonstrated a bioaccumulation factor of 27.9 for PFOS in bumblebee workers fed with sugar water containing 100 mu g/l PFOS during 5 weeks (2184 +/- A 365 ng/g BW). Finally, potential mechanisms of activity were investigated to explain the significant impact of PFOS on survival and reproduction capacity of B. terrestris. Exposure of bumblebee workers to PFOS resulted in a significant decrease in mitochondrial electron transport activity (p = 0.035) and lipid amounts (p = 0.019), while the respective p-values were 0.58 and 0.12 for protein and glucose amounts. Hence, addition of PFOS to ecdysteroid responsive Drosophila melanogaster S2 cells resulted in a strong antagonistic action on the EcR-b.act.luc reporter construct, demonstrating that PFOS may exert its effects partially through an endocrine disrupting action via the insect molting hormone or ecdysteroid receptor.},
  author       = {Mommaerts, Veerle and Hagenaars, An and Meyer, Johan and De Coen, Wim and Swevers, Luc and Mosallanejad, Hadi and Smagghe, Guy},
  issn         = {0963-9292},
  journal      = {ECOTOXICOLOGY},
  keyword      = {Bumblebee,PFOS,Survival,Reproduction,Electron transport,Energy content,Antagonistic activity,PERFLUOROOCTANE SULFONIC-ACID,DEVELOPMENTAL TOXICITY,GENE-EXPRESSION,ENALLAGMA-CYATHIGERUM,PERFLUOROALKYL ACIDS,DAPHNIA-MAGNA,LIFE-CYCLE,CELL-LINES,IN-VITRO,EXPOSURE},
  language     = {eng},
  number       = {2},
  pages        = {447--456},
  title        = {Impact of a perfluorinated organic compound PFOS on the terrestrial pollinator Bombus terrestris (Insecta, Hymenoptera)},
  url          = {http://dx.doi.org/10.1007/s10646-011-0596-2},
  volume       = {20},
  year         = {2011},
}

Chicago
Mommaerts, Veerle, An Hagenaars, Johan Meyer, Wim De Coen, Luc Swevers, Hadi Mosallanejad, and Guy Smagghe. 2011. “Impact of a Perfluorinated Organic Compound PFOS on the Terrestrial Pollinator Bombus Terrestris (Insecta, Hymenoptera).” Ecotoxicology 20 (2): 447–456.
APA
Mommaerts, V., Hagenaars, A., Meyer, J., De Coen, W., Swevers, L., Mosallanejad, H., & Smagghe, G. (2011). Impact of a perfluorinated organic compound PFOS on the terrestrial pollinator Bombus terrestris (Insecta, Hymenoptera). ECOTOXICOLOGY, 20(2), 447–456.
Vancouver
1.
Mommaerts V, Hagenaars A, Meyer J, De Coen W, Swevers L, Mosallanejad H, et al. Impact of a perfluorinated organic compound PFOS on the terrestrial pollinator Bombus terrestris (Insecta, Hymenoptera). ECOTOXICOLOGY. 2011;20(2):447–56.
MLA
Mommaerts, Veerle, An Hagenaars, Johan Meyer, et al. “Impact of a Perfluorinated Organic Compound PFOS on the Terrestrial Pollinator Bombus Terrestris (Insecta, Hymenoptera).” ECOTOXICOLOGY 20.2 (2011): 447–456. Print.