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Agrobacterium tumefaciens-mediated transformation of Artemisia annua L. and regeneration of transgenic plants

Annemieke Vergauwe UGent, Ronny Cammaert, Dirk Vandenberghe UGent, Christiane Genetello UGent, Dirk Inzé UGent, Marc Van Montagu UGent and Elfride Van den Eeckhout UGent (1996) PLANT CELL REPORTS. 15(12). p.929-933
abstract
A transformation system was developed for Artemisia annua L. plants. Leaf explants from in vitro grown plants developed callus and shoots on medium with 0.05 mg/L naphthaleneacetic acid and 0.5 mg/L N-6-benzyladenine after transformation with the C58C1 Rif(R) (pGV2260) (pTJK136) Agrobacterium tumefaciens strain. A concentration of 20 mg/L kanamycin was added in order to select transformed tissue. Kanamycin resistant shoots were rooted on naphthaleneacetic acid 0.1 mg/L. Polymerase chain reactions and DNA sequencing of the amplification products revealed that 75% of the regenerants contained the foreign genes. 94% of the transgenic plants showed a beta-glucuronidase-positive response.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
CULTURES
journal title
PLANT CELL REPORTS
Plant Cell Reports
volume
15
issue
12
pages
929 - 933
Web of Science type
Article
ISSN
0721-7714
DOI
10.1007/BF00231590
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
190115
handle
http://hdl.handle.net/1854/LU-190115
date created
2004-01-14 13:41:00
date last changed
2013-10-30 16:29:11
@article{190115,
  abstract     = {A transformation system was developed for Artemisia annua L. plants. Leaf explants from in vitro grown plants developed callus and shoots on medium with 0.05 mg/L naphthaleneacetic acid and 0.5 mg/L N-6-benzyladenine after transformation with the C58C1 Rif(R) (pGV2260) (pTJK136) Agrobacterium tumefaciens strain. A concentration of 20 mg/L kanamycin was added in order to select transformed tissue. Kanamycin resistant shoots were rooted on naphthaleneacetic acid 0.1 mg/L. Polymerase chain reactions and DNA sequencing of the amplification products revealed that 75\% of the regenerants contained the foreign genes. 94\% of the transgenic plants showed a beta-glucuronidase-positive response.},
  author       = {Vergauwe, Annemieke and Cammaert, Ronny and Vandenberghe, Dirk and Genetello, Christiane and Inz{\'e}, Dirk and Van Montagu, Marc and Van den Eeckhout, Elfride},
  issn         = {0721-7714},
  journal      = {PLANT CELL REPORTS},
  keyword      = {CULTURES},
  language     = {eng},
  number       = {12},
  pages        = {929--933},
  title        = {Agrobacterium tumefaciens-mediated transformation of Artemisia annua L. and regeneration of transgenic plants},
  url          = {http://dx.doi.org/10.1007/BF00231590},
  volume       = {15},
  year         = {1996},
}

Chicago
Vergauwe, Annemieke, Ronny Cammaert, Dirk Vandenberghe, Christiane Genetello, Dirk Inzé, Marc Van Montagu, and Elfride Van den Eeckhout. 1996. “Agrobacterium Tumefaciens-mediated Transformation of Artemisia Annua L. and Regeneration of Transgenic Plants.” Plant Cell Reports 15 (12): 929–933.
APA
Vergauwe, A., Cammaert, R., Vandenberghe, D., Genetello, C., Inzé, D., Van Montagu, M., & Van den Eeckhout, E. (1996). Agrobacterium tumefaciens-mediated transformation of Artemisia annua L. and regeneration of transgenic plants. PLANT CELL REPORTS, 15(12), 929–933.
Vancouver
1.
Vergauwe A, Cammaert R, Vandenberghe D, Genetello C, Inzé D, Van Montagu M, et al. Agrobacterium tumefaciens-mediated transformation of Artemisia annua L. and regeneration of transgenic plants. PLANT CELL REPORTS. 1996;15(12):929–33.
MLA
Vergauwe, Annemieke, Ronny Cammaert, Dirk Vandenberghe, et al. “Agrobacterium Tumefaciens-mediated Transformation of Artemisia Annua L. and Regeneration of Transgenic Plants.” PLANT CELL REPORTS 15.12 (1996): 929–933. Print.