Ghent University Academic Bibliography

@incollection{1897817,
  abstract     = {Defining protein complexes is critical to virtually all aspects of cell biology because most cellular processes are regulated by stable or more dynamic protein interactions. Elucidation of the protein-protein interaction network around transcription factors is essential to fully understand their function and regulation. In the last decade, new technologies have emerged to study protein-protein interactions under near-physiological conditions. We have developed a high-throughput tandem affinity purification (TAP)/mass spectrometry (MS) platform for cell suspension cultures to analyze protein complexes in Arabidopsis thaliana. This streamlined platform follows an integrated approach comprising generic Gateway-based vectors with high cloning flexibility, the fast generation of transgenic suspension cultures, TAP adapted for plant cells, and tandem matrix-assisted laser desorption ionization MS for the identification of purified proteins. Recently, we evaluated the GS tag, originally developed to study mammalian protein complexes, that combines two IgG-binding domains of protein G with a streptavidin-binding peptide, separated by two tobacco etch virus cleavage sites. We found that this GS tag outperforms the traditional TAP tag in plant cells, regarding both specificity and complex yield. Here, we provide detailed protocols of the GS-based TAP platform that allowed us to characterize transcription factor complexes involved in signaling in response to the plant phytohormone jasmonate.},
  author       = {Van Leene, Jelle and Eeckhout, Dominique and Persiau, Geert and Van De Slijke, Eveline and Geerinck, Jan and Van Isterdael, Gert and Witters, Erwin and De Jaeger, Geert},
  booktitle    = {Plant transcription factors : methods and procotols},
  editor       = {Yuan, Ling and Perry, Sharyn E},
  isbn         = {9781617791536},
  issn         = {1064-3745},
  keyword      = {Arabidopsis thaliana,transcription factor,cell suspension culture,protein complex,tandem affinity purification,protein interaction},
  language     = {eng},
  pages        = {195--218},
  publisher    = {Humana Press},
  series       = {Methods in Molecular Biology},
  title        = {Isolation of transcription factor complexes from Arabidopsis cell suspension cultures by tandem affinity purification},
  url          = {http://dx.doi.org/10.1007/978-1-61779-154-3\_11},
  volume       = {754},
  year         = {2011},
}

Chicago

Van Leene, Jelle, Dominique Eeckhout, Geert Persiau, Eveline Van De Slijke, Jan Geerinck, Gert Van Isterdael, Erwin Witters, and Geert De Jaeger. 2011. “Isolation of Transcription Factor Complexes from Arabidopsis Cell Suspension Cultures by Tandem Affinity Purification.” In Plant Transcription Factors : Methods and Procotols, ed. Ling Yuan and Sharyn E Perry, 754:195–218. Totowa, NJ, USA: Humana Press.

APA

Van Leene, J., Eeckhout, D., Persiau, G., Van De Slijke, E., Geerinck, J., Van Isterdael, G., Witters, E., et al. (2011). Isolation of transcription factor complexes from Arabidopsis cell suspension cultures by tandem affinity purification. In L. Yuan & S. E. Perry (Eds.), Plant transcription factors : methods and procotols (Vol. 754, pp. 195–218). Totowa, NJ, USA: Humana Press.

Vancouver

1.

Van Leene J, Eeckhout D, Persiau G, Van De Slijke E, Geerinck J, Van Isterdael G, et al. Isolation of transcription factor complexes from Arabidopsis cell suspension cultures by tandem affinity purification. In: Yuan L, Perry SE, editors. Plant transcription factors : methods and procotols. Totowa, NJ, USA: Humana Press; 2011. p. 195–218.

MLA

Van Leene, Jelle, Dominique Eeckhout, Geert Persiau, et al. “Isolation of Transcription Factor Complexes from Arabidopsis Cell Suspension Cultures by Tandem Affinity Purification.” Plant Transcription Factors : Methods and Procotols. Ed. Ling Yuan & Sharyn E Perry. Vol. 754. Totowa, NJ, USA: Humana Press, 2011. 195–218. Print.