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Asymmetric leaves2 and elongator, a histone acetyltransferase complex, mediate the establishment of polarity in leaves of Arabidopsis thaliana

Shoko Kojima, Mayumi Iwasaki, Hiro Takahashi, Tomoya Imai, Yoko Matsumura, Delphine Fleury, Maria Van Lijsebettens UGent, Yasunori Machida and Chiyoko Machida (2011) PLANT AND CELL PHYSIOLOGY. 52(8). p.1259-1273
abstract
Leaf primordia are generated around the shoot apical meristem. Mutation of the ASYMMETRIC LEAVES2 (AS2) gene of Arabidopsis thaliana results in defects in repression of the meristematic and indeterminate state, establishment of adaxial-abaxial polarity and left-right symmetry in leaves. AS2 represses transcription of meristem-specific class 1 KNOX homeobox genes and of the abaxial-determinant genes ETTIN/ARF3, KANADI2 and YABBY5. To clarify the role of AS2 in the establishment of leaf polarity, we isolated mutations that enhanced the polarity defects associated with as2. We describe here the enhancer-of-asymmetric-leaves-two1 (east1) mutation, which caused the formation of filamentous leaves with abaxialized epidermis on the as2-1 background. Levels of transcripts of class 1 KNOX and abaxial-determinant genes were markedly higher in as2-1 east1-1 mutant plants than in the wild-type and corresponding single-mutant plants. EAST1 encodes the histone acetyltransferase ELONGATA3 (ELO3), a component of the Elongator complex. Genetic analysis, using mutations in genes involved in the biogenesis of a trans-acting small interfering RNA (ta-siRNA), revealed that ELO3 mediated establishment of leaf polarity independently of AS2 and the ta-siRNA-related pathway. Treatment with an inhibitor of histone deacetylases (HDACs) caused additive polarity defects in as2-1 east1-1 mutant plants, suggesting the operation of an ELO3 pathway, independent of the HDAC pathway, in the determination of polarity. We propose that multiple pathways play important roles in repression of the expression of class 1 KNOX and abaxial-determinant genes in the development of the adaxial domain of leaves and, thus, in the establishment of leaf polarity.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Elongator, ELONGATA3 (ELO3), Leaf polarity, Histone acetyltransferase, Arabidopsis thaliana, ASYMMETRIC LEAVES2 (AS2), LEAF ADAXIAL IDENTITY, TRANSCRIPTIONAL ELONGATION, RIBOSOMAL-PROTEINS, POLYMERASE-II HOLOENZYME, INTERFERING RNA, CLASS IIIHD-ZIP, LATERAL ORGANS, GENE FAMILY, MUTATIONAL ANALYSIS, CELL-DIFFERENTIATION
journal title
PLANT AND CELL PHYSIOLOGY
Plant Cell Physiol.
volume
52
issue
8
pages
1259 - 1273
Web of Science type
Article
Web of Science id
000293917100001
JCR category
PLANT SCIENCES
JCR impact factor
4.702 (2011)
JCR rank
14/189 (2011)
JCR quartile
1 (2011)
ISSN
0032-0781
DOI
10.1093/pcp/pcr083
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
1896861
handle
http://hdl.handle.net/1854/LU-1896861
date created
2011-09-02 10:24:39
date last changed
2011-09-06 11:24:33
@article{1896861,
  abstract     = {Leaf primordia are generated around the shoot apical meristem. Mutation of the ASYMMETRIC LEAVES2 (AS2) gene of Arabidopsis thaliana results in defects in repression of the meristematic and indeterminate state, establishment of adaxial-abaxial polarity and left-right symmetry in leaves. AS2 represses transcription of meristem-specific class 1 KNOX homeobox genes and of the abaxial-determinant genes ETTIN/ARF3, KANADI2 and YABBY5. To clarify the role of AS2 in the establishment of leaf polarity, we isolated mutations that enhanced the polarity defects associated with as2. We describe here the enhancer-of-asymmetric-leaves-two1 (east1) mutation, which caused the formation of filamentous leaves with abaxialized epidermis on the as2-1 background. Levels of transcripts of class 1 KNOX and abaxial-determinant genes were markedly higher in as2-1 east1-1 mutant plants than in the wild-type and corresponding single-mutant plants. EAST1 encodes the histone acetyltransferase ELONGATA3 (ELO3), a component of the Elongator complex. Genetic analysis, using mutations in genes involved in the biogenesis of a trans-acting small interfering RNA (ta-siRNA), revealed that ELO3 mediated establishment of leaf polarity independently of AS2 and the ta-siRNA-related pathway. Treatment with an inhibitor of histone deacetylases (HDACs) caused additive polarity defects in as2-1 east1-1 mutant plants, suggesting the operation of an ELO3 pathway, independent of the HDAC pathway, in the determination of polarity. We propose that multiple pathways play important roles in repression of the expression of class 1 KNOX and abaxial-determinant genes in the development of the adaxial domain of leaves and, thus, in the establishment of leaf polarity.},
  author       = {Kojima, Shoko and Iwasaki, Mayumi and Takahashi, Hiro and Imai, Tomoya and Matsumura, Yoko and Fleury, Delphine and Van Lijsebettens, Maria and Machida, Yasunori and Machida, Chiyoko},
  issn         = {0032-0781},
  journal      = {PLANT AND CELL PHYSIOLOGY},
  keyword      = {Elongator,ELONGATA3 (ELO3),Leaf polarity,Histone acetyltransferase,Arabidopsis thaliana,ASYMMETRIC LEAVES2 (AS2),LEAF ADAXIAL IDENTITY,TRANSCRIPTIONAL ELONGATION,RIBOSOMAL-PROTEINS,POLYMERASE-II HOLOENZYME,INTERFERING RNA,CLASS IIIHD-ZIP,LATERAL ORGANS,GENE FAMILY,MUTATIONAL ANALYSIS,CELL-DIFFERENTIATION},
  language     = {eng},
  number       = {8},
  pages        = {1259--1273},
  title        = {Asymmetric leaves2 and elongator, a histone acetyltransferase complex, mediate the establishment of polarity in leaves of Arabidopsis thaliana},
  url          = {http://dx.doi.org/10.1093/pcp/pcr083},
  volume       = {52},
  year         = {2011},
}

Chicago
Kojima, Shoko, Mayumi Iwasaki, Hiro Takahashi, Tomoya Imai, Yoko Matsumura, Delphine Fleury, Maria Van Lijsebettens, Yasunori Machida, and Chiyoko Machida. 2011. “Asymmetric Leaves2 and Elongator, a Histone Acetyltransferase Complex, Mediate the Establishment of Polarity in Leaves of Arabidopsis Thaliana.” Plant and Cell Physiology 52 (8): 1259–1273.
APA
Kojima, S., Iwasaki, M., Takahashi, H., Imai, T., Matsumura, Y., Fleury, D., Van Lijsebettens, M., et al. (2011). Asymmetric leaves2 and elongator, a histone acetyltransferase complex, mediate the establishment of polarity in leaves of Arabidopsis thaliana. PLANT AND CELL PHYSIOLOGY, 52(8), 1259–1273.
Vancouver
1.
Kojima S, Iwasaki M, Takahashi H, Imai T, Matsumura Y, Fleury D, et al. Asymmetric leaves2 and elongator, a histone acetyltransferase complex, mediate the establishment of polarity in leaves of Arabidopsis thaliana. PLANT AND CELL PHYSIOLOGY. 2011;52(8):1259–73.
MLA
Kojima, Shoko, Mayumi Iwasaki, Hiro Takahashi, et al. “Asymmetric Leaves2 and Elongator, a Histone Acetyltransferase Complex, Mediate the Establishment of Polarity in Leaves of Arabidopsis Thaliana.” PLANT AND CELL PHYSIOLOGY 52.8 (2011): 1259–1273. Print.