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The auxin signalling network translates dynamic input into robust patterning at the shoot apex

Teva Vernoux, Géraldine Brunoud, Etienne Farcot, Valérie Morin, Hilde Van Den Daele UGent, Jonathan Legrand, Marina Oliva, Pradeep Das, Antoine Larrieu and Darren Wells, et al. (2011) MOLECULAR SYSTEMS BIOLOGY. 7.
abstract
The plant hormone auxin is thought to provide positional information for patterning during development. It is still unclear, however, precisely how auxin is distributed across tissues and how the hormone is sensed in space and time. The control of gene expression in response to auxin involves a complex network of over 50 potentially interacting transcriptional activators and repressors, the auxin response factors (ARFs) and Aux/IAAs. Here, we perform a large-scale analysis of the Aux/IAA-ARF pathway in the shoot apex of Arabidopsis, where dynamic auxin-based patterning controls organogenesis. A comprehensive expression map and full interactome uncovered an unexpectedly simple distribution and structure of this pathway in the shoot apex. A mathematical model of the Aux/IAA-ARF network predicted a strong buffering capacity along with spatial differences in auxin sensitivity. We then tested and confirmed these predictions using a novel auxin signalling sensor that reports input into the signalling pathway, in conjunction with the published DR5 transcriptional output reporter. Our results provide evidence that the auxin signalling network is essential to create robust patterns at the shoot apex.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
TRANSPORT, APICAL MERISTEM, AUX/IAA GENES, GENE-EXPRESSION, PLANT DEVELOPMENT, RESPONSE FACTORS, ARABIDOPSIS-THALIANA, TIME RT-PCR, BOX PROTEIN TIR1, PHYLLOTAXIS, auxin, biosensor, live imaging, ODE, signalling
journal title
MOLECULAR SYSTEMS BIOLOGY
Mol. Syst. Biol.
volume
7
article_number
508
pages
15 pages
Web of Science type
Article
Web of Science id
000293351900004
JCR category
BIOCHEMISTRY & MOLECULAR BIOLOGY
JCR impact factor
8.626 (2011)
JCR rank
24/286 (2011)
JCR quartile
1 (2011)
ISSN
1744-4292
DOI
10.1038/msb.2011.39
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1893722
handle
http://hdl.handle.net/1854/LU-1893722
date created
2011-08-26 17:27:34
date last changed
2011-08-29 14:22:14
@article{1893722,
  abstract     = {The plant hormone auxin is thought to provide positional information for patterning during development. It is still unclear, however, precisely how auxin is distributed across tissues and how the hormone is sensed in space and time. The control of gene expression in response to auxin involves a complex network of over 50 potentially interacting transcriptional activators and repressors, the auxin response factors (ARFs) and Aux/IAAs. Here, we perform a large-scale analysis of the Aux/IAA-ARF pathway in the shoot apex of Arabidopsis, where dynamic auxin-based patterning controls organogenesis. A comprehensive expression map and full interactome uncovered an unexpectedly simple distribution and structure of this pathway in the shoot apex. A mathematical model of the Aux/IAA-ARF network predicted a strong buffering capacity along with spatial differences in auxin sensitivity. We then tested and confirmed these predictions using a novel auxin signalling sensor that reports input into the signalling pathway, in conjunction with the published DR5 transcriptional output reporter. Our results provide evidence that the auxin signalling network is essential to create robust patterns at the shoot apex.},
  articleno    = {508},
  author       = {Vernoux, Teva and Brunoud, G{\'e}raldine and Farcot, Etienne and Morin, Val{\'e}rie and Van Den Daele, Hilde and Legrand, Jonathan and Oliva, Marina and Das, Pradeep and Larrieu, Antoine and Wells, Darren and Gu{\'e}don, Yann and Armitage, Lynne and Picard, Franck and Guyomarc'h, Soazig and Cellier, Coralie and Parry, Geraint and Koumproglou, Rachil and Doonan, John H and Estelle, Mark and Godin, Christophe and Kepinski, Stefan and Bennett, Malcolm and De Veylder, Lieven and Traas, Jan},
  issn         = {1744-4292},
  journal      = {MOLECULAR SYSTEMS BIOLOGY},
  keyword      = {TRANSPORT,APICAL MERISTEM,AUX/IAA GENES,GENE-EXPRESSION,PLANT DEVELOPMENT,RESPONSE FACTORS,ARABIDOPSIS-THALIANA,TIME RT-PCR,BOX PROTEIN TIR1,PHYLLOTAXIS,auxin,biosensor,live imaging,ODE,signalling},
  language     = {eng},
  pages        = {15},
  title        = {The auxin signalling network translates dynamic input into robust patterning at the shoot apex},
  url          = {http://dx.doi.org/10.1038/msb.2011.39},
  volume       = {7},
  year         = {2011},
}

Chicago
Vernoux, Teva, Géraldine Brunoud, Etienne Farcot, Valérie Morin, Hilde Van Den Daele, Jonathan Legrand, Marina Oliva, et al. 2011. “The Auxin Signalling Network Translates Dynamic Input into Robust Patterning at the Shoot Apex.” Molecular Systems Biology 7.
APA
Vernoux, T., Brunoud, G., Farcot, E., Morin, V., Van Den Daele, H., Legrand, J., Oliva, M., et al. (2011). The auxin signalling network translates dynamic input into robust patterning at the shoot apex. MOLECULAR SYSTEMS BIOLOGY, 7.
Vancouver
1.
Vernoux T, Brunoud G, Farcot E, Morin V, Van Den Daele H, Legrand J, et al. The auxin signalling network translates dynamic input into robust patterning at the shoot apex. MOLECULAR SYSTEMS BIOLOGY. 2011;7.
MLA
Vernoux, Teva, Géraldine Brunoud, Etienne Farcot, et al. “The Auxin Signalling Network Translates Dynamic Input into Robust Patterning at the Shoot Apex.” MOLECULAR SYSTEMS BIOLOGY 7 (2011): n. pag. Print.