Ghent University Academic Bibliography

Advanced

Model-based analysis of arabidopsis leaf epidermal cells reveals distinct division and expansion patterns for pavement and guard cells

Leila Kheibarshekan Asl UGent, Stijn Dhondt UGent, Véronique Boudolf UGent, Gerrit Beemster UGent, Tom Beeckman UGent, Dirk Inzé UGent, Willy Govaerts UGent and Lieven De Veylder UGent (2011) PLANT PHYSIOLOGY. 156(4). p.2172-2183
abstract
To efficiently capture sunlight for photosynthesis, leaves typically develop into a flat and thin structure. This development is driven by cell division and expansion, but the individual contribution of these processes is currently unknown, mainly because of the experimental difficulties to disentangle them in a developing organ, due to their tight interconnection. To circumvent this problem, we built a mathematic model that describes the possible division patterns and expansion rates for individual epidermal cells. This model was used to fit experimental data on cell numbers and sizes obtained over time intervals of 1 d throughout the development of the first leaf pair of Arabidopsis (Arabidopsis thaliana). The parameters were obtained by a derivative-free optimization method that minimizes the differences between the predicted and experimentally observed cell size distributions. The model allowed us to calculate probabilities for a cell to divide into guard or pavement cells, the maximum size at which it can divide, and its average cell division and expansion rates at each point during the leaf developmental process. Surprisingly, average cell cycle duration remained constant throughout leaf development, whereas no evidence for a maximum cell size threshold for cell division of pavement cells was found. Furthermore, the model predicted that neighboring cells of different sizes within the epidermis expand at distinctly different relative rates, which could be verified by direct observations. We conclude that cell division seems to occur independently from the status of cell expansion, whereas the cell cycle might act as a timer rather than as a size-regulated machinery.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
SIZE, MUTANTS, THALIANA, LEAVES, MORPHOGENESIS, CYCLE, PLANT-GROWTH, LATERAL ORGANS, GENE-EXPRESSION, ROOT-MERISTEM
journal title
PLANT PHYSIOLOGY
Plant Physiol.
volume
156
issue
4
pages
2172 - 2183
Web of Science type
Article
Web of Science id
000293568800039
JCR category
PLANT SCIENCES
JCR impact factor
6.535 (2011)
JCR rank
7/189 (2011)
JCR quartile
1 (2011)
ISSN
0032-0889
DOI
10.1104/pp.111.181180
project
Biotechnology for a sustainable economy (Bio-Economy)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1893677
handle
http://hdl.handle.net/1854/LU-1893677
date created
2011-08-26 17:02:53
date last changed
2016-12-19 15:45:56
@article{1893677,
  abstract     = {To efficiently capture sunlight for photosynthesis, leaves typically develop into a flat and thin structure. This development is driven by cell division and expansion, but the individual contribution of these processes is currently unknown, mainly because of the experimental difficulties to disentangle them in a developing organ, due to their tight interconnection. To circumvent this problem, we built a mathematic model that describes the possible division patterns and expansion rates for individual epidermal cells. This model was used to fit experimental data on cell numbers and sizes obtained over time intervals of 1 d throughout the development of the first leaf pair of Arabidopsis (Arabidopsis thaliana). The parameters were obtained by a derivative-free optimization method that minimizes the differences between the predicted and experimentally observed cell size distributions. The model allowed us to calculate probabilities for a cell to divide into guard or pavement cells, the maximum size at which it can divide, and its average cell division and expansion rates at each point during the leaf developmental process. Surprisingly, average cell cycle duration remained constant throughout leaf development, whereas no evidence for a maximum cell size threshold for cell division of pavement cells was found. Furthermore, the model predicted that neighboring cells of different sizes within the epidermis expand at distinctly different relative rates, which could be verified by direct observations. We conclude that cell division seems to occur independently from the status of cell expansion, whereas the cell cycle might act as a timer rather than as a size-regulated machinery.},
  author       = {Kheibarshekan Asl, Leila and Dhondt, Stijn and Boudolf, V{\'e}ronique and Beemster, Gerrit and Beeckman, Tom and Inz{\'e}, Dirk and Govaerts, Willy and De Veylder, Lieven},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  keyword      = {SIZE,MUTANTS,THALIANA,LEAVES,MORPHOGENESIS,CYCLE,PLANT-GROWTH,LATERAL ORGANS,GENE-EXPRESSION,ROOT-MERISTEM},
  language     = {eng},
  number       = {4},
  pages        = {2172--2183},
  title        = {Model-based analysis of arabidopsis leaf epidermal cells reveals distinct division and expansion patterns for pavement and guard cells},
  url          = {http://dx.doi.org/10.1104/pp.111.181180},
  volume       = {156},
  year         = {2011},
}

Chicago
Kheibarshekan Asl, Leila, Stijn Dhondt, Véronique Boudolf, Gerrit Beemster, Tom Beeckman, Dirk Inzé, Willy Govaerts, and Lieven De Veylder. 2011. “Model-based Analysis of Arabidopsis Leaf Epidermal Cells Reveals Distinct Division and Expansion Patterns for Pavement and Guard Cells.” Plant Physiology 156 (4): 2172–2183.
APA
Kheibarshekan Asl, L., Dhondt, S., Boudolf, V., Beemster, G., Beeckman, T., Inzé, D., Govaerts, W., et al. (2011). Model-based analysis of arabidopsis leaf epidermal cells reveals distinct division and expansion patterns for pavement and guard cells. PLANT PHYSIOLOGY, 156(4), 2172–2183.
Vancouver
1.
Kheibarshekan Asl L, Dhondt S, Boudolf V, Beemster G, Beeckman T, Inzé D, et al. Model-based analysis of arabidopsis leaf epidermal cells reveals distinct division and expansion patterns for pavement and guard cells. PLANT PHYSIOLOGY. 2011;156(4):2172–83.
MLA
Kheibarshekan Asl, Leila, Stijn Dhondt, Véronique Boudolf, et al. “Model-based Analysis of Arabidopsis Leaf Epidermal Cells Reveals Distinct Division and Expansion Patterns for Pavement and Guard Cells.” PLANT PHYSIOLOGY 156.4 (2011): 2172–2183. Print.