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Mechanism of age-dependent involution in embryonic chick notochords

E Ghanem, Maria Cornelissen (UGent) , Hubert Thierens (UGent) and Leo De Ridder (UGent)
(1996) EXPERIENTIA. 52(7). p.683-686
Author
Organization
Abstract
To study the possible mechanism of the age-dependent involution of the notochord, isolated mesenchyme-free notochords of chick embryos were cultured in vitro and compared with their counterparts in vivo. Two different aspects were evaluated: (1) DNA synthesis measured by [H-3]thymidine incorporation and visualized by autoradiography and (2) cell death quantified by counting the number of pyknotic nuclei. The results demonstrate that [H-3]thymidine uptake by notochords shows an age-dependent decrease in vitro as well as in vivo. The number of [H-3]thymidine-labelled notochord cells, however, is higher in vitro than in vivo. At the same time, there is an age-dependent increase in pyknosis in the notochord in vivo and in vitro. So, during the aging process, the number of both pyknotic nuclei and of [H-3]thymidine-labelled nuclei suggest a high turnover of notochord cells in vitro. From these results, we can conclude that the process of involution in aging notochord seems to be controlled by a programmed intrinsic process, which might be influenced partially by the microenvironment in vivo.
Keywords
[H-3] thymidine incorporation, notochord, pyknosis, age-dependent involution

Citation

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Chicago
Ghanem, E, Maria Cornelissen, Hubert Thierens, and Leo De Ridder. 1996. “Mechanism of Age-dependent Involution in Embryonic Chick Notochords.” Experientia 52 (7): 683–686.
APA
Ghanem, E., Cornelissen, M., Thierens, H., & De Ridder, L. (1996). Mechanism of age-dependent involution in embryonic chick notochords. EXPERIENTIA, 52(7), 683–686.
Vancouver
1.
Ghanem E, Cornelissen M, Thierens H, De Ridder L. Mechanism of age-dependent involution in embryonic chick notochords. EXPERIENTIA. 1996;52(7):683–6.
MLA
Ghanem, E, Maria Cornelissen, Hubert Thierens, et al. “Mechanism of Age-dependent Involution in Embryonic Chick Notochords.” EXPERIENTIA 52.7 (1996): 683–686. Print.
@article{189253,
  abstract     = {To study the possible mechanism of the age-dependent involution of the notochord, isolated mesenchyme-free notochords of chick embryos were cultured in vitro and compared with their counterparts in vivo. Two different aspects were evaluated: (1) DNA synthesis measured by [H-3]thymidine incorporation and visualized by autoradiography and (2) cell death quantified by counting the number of pyknotic nuclei. The results demonstrate that [H-3]thymidine uptake by notochords shows an age-dependent decrease in vitro as well as in vivo. The number of [H-3]thymidine-labelled notochord cells, however, is higher in vitro than in vivo. At the same time, there is an age-dependent increase in pyknosis in the notochord in vivo and in vitro. So, during the aging process, the number of both pyknotic nuclei and of [H-3]thymidine-labelled nuclei suggest a high turnover of notochord cells in vitro. From these results, we can conclude that the process of involution in aging notochord seems to be controlled by a programmed intrinsic process, which might be influenced partially by the microenvironment in vivo.},
  author       = {Ghanem, E and Cornelissen, Maria and Thierens, Hubert and De Ridder, Leo},
  issn         = {0014-4754},
  journal      = {EXPERIENTIA},
  keyword      = {[H-3] thymidine incorporation,notochord,pyknosis,age-dependent involution},
  language     = {eng},
  number       = {7},
  pages        = {683--686},
  title        = {Mechanism of age-dependent involution in embryonic chick notochords},
  url          = {http://dx.doi.org/10.1007/BF01925574},
  volume       = {52},
  year         = {1996},
}

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