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Distribution and concentrations of 5-aminosalicylic acid in rectosigmoid biopsy specimens after rectal administration

(1996) DISEASES OF THE COLON & RECTUM. 39(7). p.788-793
Author
Organization
Abstract
PURPOSE: Using the autofluorescent properties of 5-aminosalicylic acid (5-ASA), we studied the penetration and distribution of this molecule in human colonic biopsies at different time intervals after administration of 5-ASA enemas. METHODS: Fluorescence scores of rectosigmoidal biopsy specimens were compared with 5-ASA and acetyl-5-aminosalicylic (Ac-S-ASA) concentrations, determined by high-performance liquid chromatography, in adjacent biopsies and in serum samples. RESULTS: 5-ASA penetrates into the rectal mucosa and into the epithelial cells after local application by means of an enema. We found a characteristic 5-ASA staining of two intramucosal structures that need further identification: intraepithelial triangular configurations and ''5-ASA scavengers'' in the lamina propria. Fluorescence scores correlate well with 5-ASA concentrations in adjacent biopsies (r = 0.67; P < 0.005) and correlate even better with serum concentrations of 5-ASA (r = 0.84; P < 0.005) and Ac-5-ASA (r = 0.80; P < 0.005), hence reflecting the amount of systemically absorbed and metabolized 5-ASA. CONCLUSION: 5-ASA penetrates into the rectal mucosa after local application. Local availability, assessed by means of fluorescence microscopy, correlates well with serum concentrations.
Keywords
ACETYLATION, ULCERATIVE-COLITIS, PROCTITIS, INFLAMMATORY BOWEL-DISEASE, autofluorescence, rectosigmoid, enema, 5-aminosalicylic acid

Citation

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Chicago
Schoonjans, Renaat, Martine De Vos, A-M Schelfhout, Marleen Praet, and André Elewaut. 1996. “Distribution and Concentrations of 5-aminosalicylic Acid in Rectosigmoid Biopsy Specimens After Rectal Administration.” Diseases of the Colon & Rectum 39 (7): 788–793.
APA
Schoonjans, Renaat, De Vos, M., Schelfhout, A.-M., Praet, M., & Elewaut, A. (1996). Distribution and concentrations of 5-aminosalicylic acid in rectosigmoid biopsy specimens after rectal administration. DISEASES OF THE COLON & RECTUM, 39(7), 788–793.
Vancouver
1.
Schoonjans R, De Vos M, Schelfhout A-M, Praet M, Elewaut A. Distribution and concentrations of 5-aminosalicylic acid in rectosigmoid biopsy specimens after rectal administration. DISEASES OF THE COLON & RECTUM. 1996;39(7):788–93.
MLA
Schoonjans, Renaat, Martine De Vos, A-M Schelfhout, et al. “Distribution and Concentrations of 5-aminosalicylic Acid in Rectosigmoid Biopsy Specimens After Rectal Administration.” DISEASES OF THE COLON & RECTUM 39.7 (1996): 788–793. Print.
@article{189175,
  abstract     = {PURPOSE: Using the autofluorescent properties of 5-aminosalicylic acid (5-ASA), we studied the penetration and distribution of this molecule in human colonic biopsies at different time intervals after administration of 5-ASA enemas.
METHODS: Fluorescence scores of rectosigmoidal biopsy specimens were compared with 5-ASA and acetyl-5-aminosalicylic (Ac-S-ASA) concentrations, determined by high-performance liquid chromatography, in adjacent biopsies and in serum samples.
RESULTS: 5-ASA penetrates into the rectal mucosa and into the epithelial cells after local application by means of an enema. We found a characteristic 5-ASA staining of two intramucosal structures that need further identification: intraepithelial triangular configurations and ''5-ASA scavengers'' in the lamina propria. Fluorescence scores correlate well with 5-ASA concentrations in adjacent biopsies (r = 0.67; P {\textlangle} 0.005) and correlate even better with serum concentrations of 5-ASA (r = 0.84; P {\textlangle} 0.005) and Ac-5-ASA (r = 0.80; P {\textlangle} 0.005), hence reflecting the amount of systemically absorbed and metabolized 5-ASA.
CONCLUSION: 5-ASA penetrates into the rectal mucosa after local application. Local availability, assessed by means of fluorescence microscopy, correlates well with serum concentrations.},
  author       = {Schoonjans, Renaat and De Vos, Martine and Schelfhout, A-M and Praet, Marleen and Elewaut, Andr{\'e}},
  issn         = {0012-3706},
  journal      = {DISEASES OF THE COLON \& RECTUM},
  language     = {eng},
  number       = {7},
  pages        = {788--793},
  title        = {Distribution and concentrations of 5-aminosalicylic acid in rectosigmoid biopsy specimens after rectal administration},
  url          = {http://dx.doi.org/10.1007/BF02054445},
  volume       = {39},
  year         = {1996},
}

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