Toward clinical proteomics on a next-generation sequencing platform
- Author
- Daniel J Turner, Robin Tuytten, Kris PF Janssen, Jeroen Lammertyn, Jan Wuyts, Jeroen Pollet, Sven Eyckerman (UGent) , Clive Brown and Koen Kas (UGent)
- Organization
- Abstract
- We report the first next generation sequencing (NGS) application to identify and quantify proteins. Customization of protein specific aptamers enabled direct conversion of serum protein information into NGS read outs. The intrinsic ability of aptamer sequencing to highly multiplex protein detection and quantification, together with the prospect of DNA sequencing further evolving into a commodity technology, could constitute the core of a novel, universal diagnostics paradigm.
- Keywords
- GENOME, MOLECULES, SELECTION, LIGANDS, RNA-SEQ, NON-SELEX, DNA APTAMERS, SMART APTAMERS, AMPLIFICATION-FREE, CAPILLARY-ELECTROPHORESIS
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-1891054
- MLA
- Turner, Daniel J., et al. “Toward Clinical Proteomics on a Next-Generation Sequencing Platform.” ANALYTICAL CHEMISTRY, vol. 83, no. 3, 2011, pp. 666–70, doi:10.1021/ac102666n.
- APA
- Turner, D. J., Tuytten, R., Janssen, K. P., Lammertyn, J., Wuyts, J., Pollet, J., … Kas, K. (2011). Toward clinical proteomics on a next-generation sequencing platform. ANALYTICAL CHEMISTRY, 83(3), 666–670. https://doi.org/10.1021/ac102666n
- Chicago author-date
- Turner, Daniel J, Robin Tuytten, Kris PF Janssen, Jeroen Lammertyn, Jan Wuyts, Jeroen Pollet, Sven Eyckerman, Clive Brown, and Koen Kas. 2011. “Toward Clinical Proteomics on a Next-Generation Sequencing Platform.” ANALYTICAL CHEMISTRY 83 (3): 666–70. https://doi.org/10.1021/ac102666n.
- Chicago author-date (all authors)
- Turner, Daniel J, Robin Tuytten, Kris PF Janssen, Jeroen Lammertyn, Jan Wuyts, Jeroen Pollet, Sven Eyckerman, Clive Brown, and Koen Kas. 2011. “Toward Clinical Proteomics on a Next-Generation Sequencing Platform.” ANALYTICAL CHEMISTRY 83 (3): 666–670. doi:10.1021/ac102666n.
- Vancouver
- 1.Turner DJ, Tuytten R, Janssen KP, Lammertyn J, Wuyts J, Pollet J, et al. Toward clinical proteomics on a next-generation sequencing platform. ANALYTICAL CHEMISTRY. 2011;83(3):666–70.
- IEEE
- [1]D. J. Turner et al., “Toward clinical proteomics on a next-generation sequencing platform,” ANALYTICAL CHEMISTRY, vol. 83, no. 3, pp. 666–670, 2011.
@article{1891054, abstract = {{We report the first next generation sequencing (NGS) application to identify and quantify proteins. Customization of protein specific aptamers enabled direct conversion of serum protein information into NGS read outs. The intrinsic ability of aptamer sequencing to highly multiplex protein detection and quantification, together with the prospect of DNA sequencing further evolving into a commodity technology, could constitute the core of a novel, universal diagnostics paradigm.}}, author = {{Turner, Daniel J and Tuytten, Robin and Janssen, Kris PF and Lammertyn, Jeroen and Wuyts, Jan and Pollet, Jeroen and Eyckerman, Sven and Brown, Clive and Kas, Koen}}, issn = {{0003-2700}}, journal = {{ANALYTICAL CHEMISTRY}}, keywords = {{GENOME,MOLECULES,SELECTION,LIGANDS,RNA-SEQ,NON-SELEX,DNA APTAMERS,SMART APTAMERS,AMPLIFICATION-FREE,CAPILLARY-ELECTROPHORESIS}}, language = {{eng}}, number = {{3}}, pages = {{666--670}}, title = {{Toward clinical proteomics on a next-generation sequencing platform}}, url = {{http://doi.org/10.1021/ac102666n}}, volume = {{83}}, year = {{2011}}, }
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