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Dysbiosis of the faecal microbiota in patients with Crohn's disease and their unaffected relatives

Marie Joossens, Geert Huys UGent, Margo Cnockaert UGent, Vicky De Preter, Kristin Verbeke, Paul Rutgeerts, Peter Vandamme UGent and Severine Vermeire (2011) GUT. 60(5). p.631-637
abstract
Background and aims A general dysbiosis of the intestinal microbiota has been established in patients with Crohn's disease (CD), but a systematic characterisation of this dysbiosis is lacking. Therefore the composition of the predominant faecal microbiota of patients with CD was studied in comparison with the predominant composition in unaffected controls. Whether dysbiosis is present in relatives of patients CD was also examined. Methods Focusing on families with at least three members affected with CD, faecal samples of 68 patients with CD, 84 of their unaffected relatives and 55 matched controls were subjected to community fingerprinting of the predominant microbiota using denaturing gradient gel electrophoresis (DGGE). To analyse the DGGE profiles, BioNumerics software and non-parametric statistical analyses (SPSS V. 17.0) were used. Observed differences in the predominant microbiota were subsequently confirmed and quantified with real-time PCR. Results Five bacterial species characterised dysbiosis in CD, namely a decrease in Dialister invisus (p = 0.04), an uncharacterised species of Clostridium cluster XIVa (p = 0.03), Faecalibacterium prausnitzii (p<1.3x10(-5)) and Bifidobacterium adolescentis (p = 5.4x10(-6)), and an increase in Ruminococcus gnavus (p = 2.1x10(-7)). Unaffected relatives of patients with CD had less Collinsella aerofaciens (p = 0.004) and a member of the Escherichia coli-Shigella group (p = 0.01) and more Ruminococcus torques (p = 0.02) in their predominant microbiota as compared with healthy subjects. Conclusion Unaffected relatives of patients with CD have a different composition of their microbiota compared with healthy controls. This dysbiosis is not characterised by lack of butyrate producing-bacteria as observed in CD but suggests a role for microorganisms with mucin degradation capacity.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
POLYMORPHISM, ASSOCIATION, HOST, FLORA, MUCOSA, BACTERIA, SUSCEPTIBILITY, ESCHERICHIA-COLI, GRADIENT GEL-ELECTROPHORESIS, INFLAMMATORY-BOWEL-DISEASE
journal title
GUT
Gut
volume
60
issue
5
pages
631 - 637
Web of Science type
Article
Web of Science id
000289076700009
JCR category
GASTROENTEROLOGY & HEPATOLOGY
JCR impact factor
10.111 (2011)
JCR rank
3/73 (2011)
JCR quartile
1 (2011)
ISSN
0017-5749
DOI
10.1136/gut.2010.223263
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1889818
handle
http://hdl.handle.net/1854/LU-1889818
date created
2011-08-17 16:03:06
date last changed
2011-08-22 13:13:39
@article{1889818,
  abstract     = {Background and aims A general dysbiosis of the intestinal microbiota has been established in patients with Crohn's disease (CD), but a systematic characterisation of this dysbiosis is lacking. Therefore the composition of the predominant faecal microbiota of patients with CD was studied in comparison with the predominant composition in unaffected controls. Whether dysbiosis is present in relatives of patients CD was also examined. Methods Focusing on families with at least three members affected with CD, faecal samples of 68 patients with CD, 84 of their unaffected relatives and 55 matched controls were subjected to community fingerprinting of the predominant microbiota using denaturing gradient gel electrophoresis (DGGE). To analyse the DGGE profiles, BioNumerics software and non-parametric statistical analyses (SPSS V. 17.0) were used. Observed differences in the predominant microbiota were subsequently confirmed and quantified with real-time PCR. Results Five bacterial species characterised dysbiosis in CD, namely a decrease in Dialister invisus (p = 0.04), an uncharacterised species of Clostridium cluster XIVa (p = 0.03), Faecalibacterium prausnitzii (p{\textlangle}1.3x10(-5)) and Bifidobacterium adolescentis (p = 5.4x10(-6)), and an increase in Ruminococcus gnavus (p = 2.1x10(-7)). Unaffected relatives of patients with CD had less Collinsella aerofaciens (p = 0.004) and a member of the Escherichia coli-Shigella group (p = 0.01) and more Ruminococcus torques (p = 0.02) in their predominant microbiota as compared with healthy subjects. Conclusion Unaffected relatives of patients with CD have a different composition of their microbiota compared with healthy controls. This dysbiosis is not characterised by lack of butyrate producing-bacteria as observed in CD but suggests a role for microorganisms with mucin degradation capacity.},
  author       = {Joossens, Marie and Huys, Geert and Cnockaert, Margo and De Preter, Vicky and Verbeke, Kristin and Rutgeerts, Paul and Vandamme, Peter and Vermeire, Severine},
  issn         = {0017-5749},
  journal      = {GUT},
  keyword      = {POLYMORPHISM,ASSOCIATION,HOST,FLORA,MUCOSA,BACTERIA,SUSCEPTIBILITY,ESCHERICHIA-COLI,GRADIENT GEL-ELECTROPHORESIS,INFLAMMATORY-BOWEL-DISEASE},
  language     = {eng},
  number       = {5},
  pages        = {631--637},
  title        = {Dysbiosis of the faecal microbiota in patients with Crohn's disease and their unaffected relatives},
  url          = {http://dx.doi.org/10.1136/gut.2010.223263},
  volume       = {60},
  year         = {2011},
}

Chicago
Joossens, Marie, Geert Huys, Margo Cnockaert, Vicky De Preter, Kristin Verbeke, Paul Rutgeerts, Peter Vandamme, and Severine Vermeire. 2011. “Dysbiosis of the Faecal Microbiota in Patients with Crohn’s Disease and Their Unaffected Relatives.” GUT 60 (5): 631–637.
APA
Joossens, M., Huys, G., Cnockaert, M., De Preter, V., Verbeke, K., Rutgeerts, P., Vandamme, P., et al. (2011). Dysbiosis of the faecal microbiota in patients with Crohn’s disease and their unaffected relatives. GUT, 60(5), 631–637.
Vancouver
1.
Joossens M, Huys G, Cnockaert M, De Preter V, Verbeke K, Rutgeerts P, et al. Dysbiosis of the faecal microbiota in patients with Crohn’s disease and their unaffected relatives. GUT. 2011;60(5):631–7.
MLA
Joossens, Marie, Geert Huys, Margo Cnockaert, et al. “Dysbiosis of the Faecal Microbiota in Patients with Crohn’s Disease and Their Unaffected Relatives.” GUT 60.5 (2011): 631–637. Print.