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Effect of lactobacilli on the ecology of the gastro-intestinal microbiota cultured in the SHIME reactor

Koen Molly, Ingrid De Smet, Lode Nollet, Marleen Vande Woestyne and Willy Verstraete UGent (1996) MICROBIAL ECOLOGY IN HEALTH AND DISEASE. 9(2). p.79-89
abstract
Evidence is presented that an established bacterial gastro-intestinal community can undergo significant changes upon addition of lactobacilli. Indeed, administration of doses of about log(10) 8-9 CFU/day were shown to alter the gastro-intestinal community both in terms of population levels and biochemical activities. Four bacterial strains, namely two Lactobacillus plantarum species, Escherichia coli and Aeromonas hydrophila, were added separately to the SHIME reactor, an in vitro simulation of the human intestinal microbial ecosystem. The survival of these strains and their effect on the performance of the microbial ecosystem were studied. The experiments carried out with the L. plantarum strains revealed significant decreases of CH4-production, beta-glucuronidase activity and presence of total anaerobes, methanogens, gram-negative aerobes and anaerobes and enterobacteriaceae. However, within two weeks after the Lactobacillus supplementation was stopped, the ecological balance re-established towards the original microbiota. The results suggest that if these types of Lactobacillus strains were to be used as a probiotic, they should be supplemented daily at doses of 10(8) per capita to obtain a prolonged influence on the intestinal microbiota. Neither of the other strains had a significant effect on methane production or bacterial numbers of methanogens. During the administration of Escherichia coli, the beta-glucuronidase activity increased.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
in vitro simulation, lactobacilli, intestinal microbiota, inoculation efficiency, LACTIC-ACID BACTERIA, ACIDOPHILUS, COLONIZATION, CHOLESTEROL, PLANTARUM, GENE, MILK
journal title
MICROBIAL ECOLOGY IN HEALTH AND DISEASE
Microb. Ecol. Health Dis.
volume
9
issue
2
pages
79 - 89
Web of Science type
Article
ISSN
0891-060X
DOI
10.1002/(SICI)1234-987X(199603)9:2<79::AID-MEH415>3.3.CO;2-V
language
English
UGent publication?
yes
classification
A1
id
188903
handle
http://hdl.handle.net/1854/LU-188903
date created
2004-01-14 13:41:00
date last changed
2017-04-27 12:46:17
@article{188903,
  abstract     = {Evidence is presented that an established bacterial gastro-intestinal community can undergo significant changes upon addition of lactobacilli. Indeed, administration of doses of about log(10) 8-9 CFU/day were shown to alter the gastro-intestinal community both in terms of population levels and biochemical activities. 
Four bacterial strains, namely two Lactobacillus plantarum species, Escherichia coli and Aeromonas hydrophila, were added separately to the SHIME reactor, an in vitro simulation of the human intestinal microbial ecosystem. The survival of these strains and their effect on the performance of the microbial ecosystem were studied. The experiments carried out with the L. plantarum strains revealed significant decreases of CH4-production, beta-glucuronidase activity and presence of total anaerobes, methanogens, gram-negative aerobes and anaerobes and enterobacteriaceae. However, within two weeks after the Lactobacillus supplementation was stopped, the ecological balance re-established towards the original microbiota. The results suggest that if these types of Lactobacillus strains were to be used as a probiotic, they should be supplemented daily at doses of 10(8) per capita to obtain a prolonged influence on the intestinal microbiota. Neither of the other strains had a significant effect on methane production or bacterial numbers of methanogens. During the administration of Escherichia coli, the beta-glucuronidase activity increased.},
  author       = {Molly, Koen and De Smet, Ingrid and Nollet, Lode and Vande Woestyne, Marleen and Verstraete, Willy},
  issn         = {0891-060X},
  journal      = {MICROBIAL ECOLOGY IN HEALTH AND DISEASE},
  keyword      = {in vitro simulation,lactobacilli,intestinal microbiota,inoculation efficiency,LACTIC-ACID BACTERIA,ACIDOPHILUS,COLONIZATION,CHOLESTEROL,PLANTARUM,GENE,MILK},
  language     = {eng},
  number       = {2},
  pages        = {79--89},
  title        = {Effect of lactobacilli on the ecology of the gastro-intestinal microbiota cultured in the SHIME reactor},
  url          = {http://dx.doi.org/10.1002/(SICI)1234-987X(199603)9:2{\textlangle}79::AID-MEH415{\textrangle}3.3.CO;2-V},
  volume       = {9},
  year         = {1996},
}

Chicago
Molly, Koen, Ingrid De Smet, Lode Nollet, Marleen Vande Woestyne, and Willy Verstraete. 1996. “Effect of Lactobacilli on the Ecology of the Gastro-intestinal Microbiota Cultured in the SHIME Reactor.” Microbial Ecology in Health and Disease 9 (2): 79–89.
APA
Molly, K., De Smet, I., Nollet, L., Vande Woestyne, M., & Verstraete, W. (1996). Effect of lactobacilli on the ecology of the gastro-intestinal microbiota cultured in the SHIME reactor. MICROBIAL ECOLOGY IN HEALTH AND DISEASE, 9(2), 79–89.
Vancouver
1.
Molly K, De Smet I, Nollet L, Vande Woestyne M, Verstraete W. Effect of lactobacilli on the ecology of the gastro-intestinal microbiota cultured in the SHIME reactor. MICROBIAL ECOLOGY IN HEALTH AND DISEASE. 1996;9(2):79–89.
MLA
Molly, Koen, Ingrid De Smet, Lode Nollet, et al. “Effect of Lactobacilli on the Ecology of the Gastro-intestinal Microbiota Cultured in the SHIME Reactor.” MICROBIAL ECOLOGY IN HEALTH AND DISEASE 9.2 (1996): 79–89. Print.