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Transcriptional and post-transcriptional regulation of a NAC1 transcription factor in Medicago truncatula roots

(2011) NEW PHYTOLOGIST. 191(3). p.647-661
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Abstract
Legume roots develop two types of lateral organs, lateral roots and nodules. Nodules develop as a result of a symbiotic interaction with rhizobia and provide a niche for the bacteria to fix atmospheric nitrogen for the plant. The Arabidopsis NAC1 transcription factor is involved in lateral root formation, and is regulated post-transcriptionally by miRNA164 and by SINAT5-dependent ubiquitination. We analyzed in Medicago truncatula the role of the closest NAC1 homolog in lateral root formation and in nodulation. MtNAC1 shows a different expression pattern in response to auxin than its Arabidopsis homolog and no changes in lateral root number or nodulation were observed in plants affected in MtNAC1 expression. In addition, no interaction was found with SINA E3 ligases, suggesting that post-translational regulation of MtNAC1 does not occur in M. truncatula. Similar to what was found in Arabidopsis, a conserved miR164 target site was retrieved in MtNAC1, which reduced protein accumulation of a GFP-miR164 sensor. Furthermore, miR164 and MtNAC1 show an overlapping expression pattern in symbiotic nodules, and overexpression of this miRNA led to a reduction in nodule number. This work suggests that regulatory pathways controlling a conserved transcription factor are complex and divergent between M. truncatula and Arabidopsis.
Keywords
GENE-EXPRESSION, ARABIDOPSIS-THALIANA, microRNA, lateral root, miR164, nodule, SINA, PLANT MICRORNAS, NODULATION, WHITE CLOVER, AUXIN SIGNALS, ORGAN FORMATION, COMPLEX REGULATION, NODULE DEVELOPMENT, RHIZOBIA-LEGUME SYMBIOSIS

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Chicago
D’haeseleer, Katrien, Griet Den Herder, Carole Laffont, Julie Plet, Virginie Mortier, Christine Lelandais-Briere, Stefanie De Bodt, et al. 2011. “Transcriptional and Post-transcriptional Regulation of a NAC1 Transcription Factor in Medicago Truncatula Roots.” New Phytologist 191 (3): 647–661.
APA
D’haeseleer, K., Den Herder, G., Laffont, C., Plet, J., Mortier, V., Lelandais-Briere, C., De Bodt, S., et al. (2011). Transcriptional and post-transcriptional regulation of a NAC1 transcription factor in Medicago truncatula roots. NEW PHYTOLOGIST, 191(3), 647–661.
Vancouver
1.
D’haeseleer K, Den Herder G, Laffont C, Plet J, Mortier V, Lelandais-Briere C, et al. Transcriptional and post-transcriptional regulation of a NAC1 transcription factor in Medicago truncatula roots. NEW PHYTOLOGIST. 2011;191(3):647–61.
MLA
D’haeseleer, Katrien, Griet Den Herder, Carole Laffont, et al. “Transcriptional and Post-transcriptional Regulation of a NAC1 Transcription Factor in Medicago Truncatula Roots.” NEW PHYTOLOGIST 191.3 (2011): 647–661. Print.
@article{1888573,
  abstract     = {Legume roots develop two types of lateral organs, lateral roots and nodules. Nodules develop as a result of a symbiotic interaction with rhizobia and provide a niche for the bacteria to fix atmospheric nitrogen for the plant.
The Arabidopsis NAC1 transcription factor is involved in lateral root formation, and is regulated post-transcriptionally by miRNA164 and by SINAT5-dependent ubiquitination. We analyzed in Medicago truncatula the role of the closest NAC1 homolog in lateral root formation and in nodulation.
MtNAC1 shows a different expression pattern in response to auxin than its Arabidopsis homolog and no changes in lateral root number or nodulation were observed in plants affected in MtNAC1 expression. In addition, no interaction was found with SINA E3 ligases, suggesting that post-translational regulation of MtNAC1 does not occur in M. truncatula. Similar to what was found in Arabidopsis, a conserved miR164 target site was retrieved in MtNAC1, which reduced protein accumulation of a GFP-miR164 sensor. Furthermore, miR164 and MtNAC1 show an overlapping expression pattern in symbiotic nodules, and overexpression of this miRNA led to a reduction in nodule number.
This work suggests that regulatory pathways controlling a conserved transcription factor are complex and divergent between M. truncatula and Arabidopsis.},
  author       = {D'haeseleer, Katrien and Den Herder, Griet and Laffont, Carole and Plet, Julie and Mortier, Virginie and Lelandais-Briere, Christine and De Bodt, Stefanie and De Keyser, Annick and Crespi, Martin and Holsters, Marcella and Frugier, Florian and Goormachtig, Sofie},
  issn         = {0028-646X},
  journal      = {NEW PHYTOLOGIST},
  keyword      = {GENE-EXPRESSION,ARABIDOPSIS-THALIANA,microRNA,lateral root,miR164,nodule,SINA,PLANT MICRORNAS,NODULATION,WHITE CLOVER,AUXIN SIGNALS,ORGAN FORMATION,COMPLEX REGULATION,NODULE DEVELOPMENT,RHIZOBIA-LEGUME SYMBIOSIS},
  language     = {eng},
  number       = {3},
  pages        = {647--661},
  title        = {Transcriptional and post-transcriptional regulation of a NAC1 transcription factor in Medicago truncatula roots},
  url          = {http://dx.doi.org/10.1111/j.1469-8137.2011.03719.x},
  volume       = {191},
  year         = {2011},
}

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