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Transcriptional and post-transcriptional regulation of a NAC1 transcription factor in Medicago truncatula roots

Katrien D'haeseleer UGent, Griet Den Herder UGent, Carole Laffont, Julie Plet, Virginie Mortier UGent, Christine Lelandais-Briere, Stefanie De Bodt UGent, Annick De Keyser UGent, Martin Crespi and Marcella Holsters UGent, et al. (2011) NEW PHYTOLOGIST. 191(3). p.647-661
abstract
Legume roots develop two types of lateral organs, lateral roots and nodules. Nodules develop as a result of a symbiotic interaction with rhizobia and provide a niche for the bacteria to fix atmospheric nitrogen for the plant. The Arabidopsis NAC1 transcription factor is involved in lateral root formation, and is regulated post-transcriptionally by miRNA164 and by SINAT5-dependent ubiquitination. We analyzed in Medicago truncatula the role of the closest NAC1 homolog in lateral root formation and in nodulation. MtNAC1 shows a different expression pattern in response to auxin than its Arabidopsis homolog and no changes in lateral root number or nodulation were observed in plants affected in MtNAC1 expression. In addition, no interaction was found with SINA E3 ligases, suggesting that post-translational regulation of MtNAC1 does not occur in M. truncatula. Similar to what was found in Arabidopsis, a conserved miR164 target site was retrieved in MtNAC1, which reduced protein accumulation of a GFP-miR164 sensor. Furthermore, miR164 and MtNAC1 show an overlapping expression pattern in symbiotic nodules, and overexpression of this miRNA led to a reduction in nodule number. This work suggests that regulatory pathways controlling a conserved transcription factor are complex and divergent between M. truncatula and Arabidopsis.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
GENE-EXPRESSION, ARABIDOPSIS-THALIANA, microRNA, lateral root, miR164, nodule, SINA, PLANT MICRORNAS, NODULATION, WHITE CLOVER, AUXIN SIGNALS, ORGAN FORMATION, COMPLEX REGULATION, NODULE DEVELOPMENT, RHIZOBIA-LEGUME SYMBIOSIS
journal title
NEW PHYTOLOGIST
New Phytol.
volume
191
issue
3
pages
647 - 661
Web of Science type
Article
Web of Science id
000292924600007
JCR category
PLANT SCIENCES
JCR impact factor
6.645 (2011)
JCR rank
6/189 (2011)
JCR quartile
1 (2011)
ISSN
0028-646X
DOI
10.1111/j.1469-8137.2011.03719.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1888573
handle
http://hdl.handle.net/1854/LU-1888573
date created
2011-08-12 18:58:01
date last changed
2013-10-11 14:21:31
@article{1888573,
  abstract     = {Legume roots develop two types of lateral organs, lateral roots and nodules. Nodules develop as a result of a symbiotic interaction with rhizobia and provide a niche for the bacteria to fix atmospheric nitrogen for the plant.
The Arabidopsis NAC1 transcription factor is involved in lateral root formation, and is regulated post-transcriptionally by miRNA164 and by SINAT5-dependent ubiquitination. We analyzed in Medicago truncatula the role of the closest NAC1 homolog in lateral root formation and in nodulation.
MtNAC1 shows a different expression pattern in response to auxin than its Arabidopsis homolog and no changes in lateral root number or nodulation were observed in plants affected in MtNAC1 expression. In addition, no interaction was found with SINA E3 ligases, suggesting that post-translational regulation of MtNAC1 does not occur in M. truncatula. Similar to what was found in Arabidopsis, a conserved miR164 target site was retrieved in MtNAC1, which reduced protein accumulation of a GFP-miR164 sensor. Furthermore, miR164 and MtNAC1 show an overlapping expression pattern in symbiotic nodules, and overexpression of this miRNA led to a reduction in nodule number.
This work suggests that regulatory pathways controlling a conserved transcription factor are complex and divergent between M. truncatula and Arabidopsis.},
  author       = {D'haeseleer, Katrien and Den Herder, Griet and Laffont, Carole and Plet, Julie and Mortier, Virginie and Lelandais-Briere, Christine and De Bodt, Stefanie and De Keyser, Annick and Crespi, Martin and Holsters, Marcella and Frugier, Florian and Goormachtig, Sofie},
  issn         = {0028-646X},
  journal      = {NEW PHYTOLOGIST},
  keyword      = {GENE-EXPRESSION,ARABIDOPSIS-THALIANA,microRNA,lateral root,miR164,nodule,SINA,PLANT MICRORNAS,NODULATION,WHITE CLOVER,AUXIN SIGNALS,ORGAN FORMATION,COMPLEX REGULATION,NODULE DEVELOPMENT,RHIZOBIA-LEGUME SYMBIOSIS},
  language     = {eng},
  number       = {3},
  pages        = {647--661},
  title        = {Transcriptional and post-transcriptional regulation of a NAC1 transcription factor in Medicago truncatula roots},
  url          = {http://dx.doi.org/10.1111/j.1469-8137.2011.03719.x},
  volume       = {191},
  year         = {2011},
}

Chicago
D’haeseleer, Katrien, Griet Den Herder, Carole Laffont, Julie Plet, Virginie Mortier, Christine Lelandais-Briere, Stefanie De Bodt, et al. 2011. “Transcriptional and Post-transcriptional Regulation of a NAC1 Transcription Factor in Medicago Truncatula Roots.” New Phytologist 191 (3): 647–661.
APA
D’haeseleer, K., Den Herder, G., Laffont, C., Plet, J., Mortier, V., Lelandais-Briere, C., De Bodt, S., et al. (2011). Transcriptional and post-transcriptional regulation of a NAC1 transcription factor in Medicago truncatula roots. NEW PHYTOLOGIST, 191(3), 647–661.
Vancouver
1.
D’haeseleer K, Den Herder G, Laffont C, Plet J, Mortier V, Lelandais-Briere C, et al. Transcriptional and post-transcriptional regulation of a NAC1 transcription factor in Medicago truncatula roots. NEW PHYTOLOGIST. 2011;191(3):647–61.
MLA
D’haeseleer, Katrien, Griet Den Herder, Carole Laffont, et al. “Transcriptional and Post-transcriptional Regulation of a NAC1 Transcription Factor in Medicago Truncatula Roots.” NEW PHYTOLOGIST 191.3 (2011): 647–661. Print.