Advanced search
1 file | 2.08 MB Add to list

Transcriptional and post-transcriptional regulation of a NAC1 transcription factor in Medicago truncatula roots

(2011) NEW PHYTOLOGIST. 191(3). p.647-661
Author
Organization
Abstract
Legume roots develop two types of lateral organs, lateral roots and nodules. Nodules develop as a result of a symbiotic interaction with rhizobia and provide a niche for the bacteria to fix atmospheric nitrogen for the plant. The Arabidopsis NAC1 transcription factor is involved in lateral root formation, and is regulated post-transcriptionally by miRNA164 and by SINAT5-dependent ubiquitination. We analyzed in Medicago truncatula the role of the closest NAC1 homolog in lateral root formation and in nodulation. MtNAC1 shows a different expression pattern in response to auxin than its Arabidopsis homolog and no changes in lateral root number or nodulation were observed in plants affected in MtNAC1 expression. In addition, no interaction was found with SINA E3 ligases, suggesting that post-translational regulation of MtNAC1 does not occur in M. truncatula. Similar to what was found in Arabidopsis, a conserved miR164 target site was retrieved in MtNAC1, which reduced protein accumulation of a GFP-miR164 sensor. Furthermore, miR164 and MtNAC1 show an overlapping expression pattern in symbiotic nodules, and overexpression of this miRNA led to a reduction in nodule number. This work suggests that regulatory pathways controlling a conserved transcription factor are complex and divergent between M. truncatula and Arabidopsis.
Keywords
GENE-EXPRESSION, ARABIDOPSIS-THALIANA, microRNA, lateral root, miR164, nodule, SINA, PLANT MICRORNAS, NODULATION, WHITE CLOVER, AUXIN SIGNALS, ORGAN FORMATION, COMPLEX REGULATION, NODULE DEVELOPMENT, RHIZOBIA-LEGUME SYMBIOSIS

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 2.08 MB

Citation

Please use this url to cite or link to this publication:

MLA
D’haeseleer, Katrien, et al. “Transcriptional and Post-Transcriptional Regulation of a NAC1 Transcription Factor in Medicago Truncatula Roots.” NEW PHYTOLOGIST, vol. 191, no. 3, 2011, pp. 647–61, doi:10.1111/j.1469-8137.2011.03719.x.
APA
D’haeseleer, K., Den Herder, G., Laffont, C., Plet, J., Mortier, V., Lelandais-Briere, C., … Goormachtig, S. (2011). Transcriptional and post-transcriptional regulation of a NAC1 transcription factor in Medicago truncatula roots. NEW PHYTOLOGIST, 191(3), 647–661. https://doi.org/10.1111/j.1469-8137.2011.03719.x
Chicago author-date
D’haeseleer, Katrien, Griet Den Herder, Carole Laffont, Julie Plet, Virginie Mortier, Christine Lelandais-Briere, Stefanie De Bodt, et al. 2011. “Transcriptional and Post-Transcriptional Regulation of a NAC1 Transcription Factor in Medicago Truncatula Roots.” NEW PHYTOLOGIST 191 (3): 647–61. https://doi.org/10.1111/j.1469-8137.2011.03719.x.
Chicago author-date (all authors)
D’haeseleer, Katrien, Griet Den Herder, Carole Laffont, Julie Plet, Virginie Mortier, Christine Lelandais-Briere, Stefanie De Bodt, Annick De Keyser, Martin Crespi, Marcella Holsters, Florian Frugier, and Sofie Goormachtig. 2011. “Transcriptional and Post-Transcriptional Regulation of a NAC1 Transcription Factor in Medicago Truncatula Roots.” NEW PHYTOLOGIST 191 (3): 647–661. doi:10.1111/j.1469-8137.2011.03719.x.
Vancouver
1.
D’haeseleer K, Den Herder G, Laffont C, Plet J, Mortier V, Lelandais-Briere C, et al. Transcriptional and post-transcriptional regulation of a NAC1 transcription factor in Medicago truncatula roots. NEW PHYTOLOGIST. 2011;191(3):647–61.
IEEE
[1]
K. D’haeseleer et al., “Transcriptional and post-transcriptional regulation of a NAC1 transcription factor in Medicago truncatula roots,” NEW PHYTOLOGIST, vol. 191, no. 3, pp. 647–661, 2011.
@article{1888573,
  abstract     = {{Legume roots develop two types of lateral organs, lateral roots and nodules. Nodules develop as a result of a symbiotic interaction with rhizobia and provide a niche for the bacteria to fix atmospheric nitrogen for the plant.
The Arabidopsis NAC1 transcription factor is involved in lateral root formation, and is regulated post-transcriptionally by miRNA164 and by SINAT5-dependent ubiquitination. We analyzed in Medicago truncatula the role of the closest NAC1 homolog in lateral root formation and in nodulation.
MtNAC1 shows a different expression pattern in response to auxin than its Arabidopsis homolog and no changes in lateral root number or nodulation were observed in plants affected in MtNAC1 expression. In addition, no interaction was found with SINA E3 ligases, suggesting that post-translational regulation of MtNAC1 does not occur in M. truncatula. Similar to what was found in Arabidopsis, a conserved miR164 target site was retrieved in MtNAC1, which reduced protein accumulation of a GFP-miR164 sensor. Furthermore, miR164 and MtNAC1 show an overlapping expression pattern in symbiotic nodules, and overexpression of this miRNA led to a reduction in nodule number.
This work suggests that regulatory pathways controlling a conserved transcription factor are complex and divergent between M. truncatula and Arabidopsis.}},
  author       = {{D'haeseleer, Katrien and Den Herder, Griet and Laffont, Carole and Plet, Julie and Mortier, Virginie and Lelandais-Briere, Christine and De Bodt, Stefanie and De Keyser, Annick and Crespi, Martin and Holsters, Marcella and Frugier, Florian and Goormachtig, Sofie}},
  issn         = {{0028-646X}},
  journal      = {{NEW PHYTOLOGIST}},
  keywords     = {{GENE-EXPRESSION,ARABIDOPSIS-THALIANA,microRNA,lateral root,miR164,nodule,SINA,PLANT MICRORNAS,NODULATION,WHITE CLOVER,AUXIN SIGNALS,ORGAN FORMATION,COMPLEX REGULATION,NODULE DEVELOPMENT,RHIZOBIA-LEGUME SYMBIOSIS}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{647--661}},
  title        = {{Transcriptional and post-transcriptional regulation of a NAC1 transcription factor in Medicago truncatula roots}},
  url          = {{http://doi.org/10.1111/j.1469-8137.2011.03719.x}},
  volume       = {{191}},
  year         = {{2011}},
}

Altmetric
View in Altmetric
Web of Science
Times cited: