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Enrichment of a microbial community performing anaerobic oxidation of methane in a continuous high-pressure bioreactor

Yu Zhang (UGent) , Loïs Maignien (UGent) , Xianxian Zhao, Fengping Wang and Nico Boon (UGent)
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Abstract
Background: Anaerobic oxidation of methane coupled to sulphate reduction (SR-AOM) prevents more than 90% of the oceanic methane emission to the atmosphere. In a previous study, we demonstrated that the high methane pressure (1, 4.5, and 8 MPa) stimulated in vitro SR-AOM activity. However, the information on the effect of high-pressure on the microbial community structure and architecture was still lacking. Results: In this study we analysed the long-term enrichment (286 days) of this microbial community, which was mediating SR-AOM in a continuous high-pressure bioreactor. 99.7% of the total biovolume represented cells in the form of small aggregates (diameter less then 15 mu m). An increase of the total biovolume was observed (2.5 times). After 286 days, the ANME-2 (anaerobic methanotrophic archaea subgroup 2) and SRB (sulphate reducing bacteria) increased with a factor 12.5 and 8.4, respectively. Conclusion: This paper reports a net biomass growth of communities involved in SR-AOM, incubated at high-pressure.
Keywords
MARINE-SEDIMENTS, METHANOTROPHIC ARCHAEA, DIVERSITY, ABUNDANCE, GROWTH, IDENTIFICATION, MOSBY MUD VOLCANO, CONTINUOUS-FLOW BIOREACTOR, SULFATE-REDUCING BACTERIA, OXIDIZING ARCHAEA

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Chicago
Zhang, Yu, Loïs Maignien, Xianxian Zhao, Fengping Wang, and Nico Boon. 2011. “Enrichment of a Microbial Community Performing Anaerobic Oxidation of Methane in a Continuous High-pressure Bioreactor.” Bmc Microbiology 11.
APA
Zhang, Yu, Maignien, L., Zhao, X., Wang, F., & Boon, N. (2011). Enrichment of a microbial community performing anaerobic oxidation of methane in a continuous high-pressure bioreactor. BMC MICROBIOLOGY, 11.
Vancouver
1.
Zhang Y, Maignien L, Zhao X, Wang F, Boon N. Enrichment of a microbial community performing anaerobic oxidation of methane in a continuous high-pressure bioreactor. BMC MICROBIOLOGY. 2011;11.
MLA
Zhang, Yu, Loïs Maignien, Xianxian Zhao, et al. “Enrichment of a Microbial Community Performing Anaerobic Oxidation of Methane in a Continuous High-pressure Bioreactor.” BMC MICROBIOLOGY 11 (2011): n. pag. Print.
@article{1888428,
  abstract     = {Background: Anaerobic oxidation of methane coupled to sulphate reduction (SR-AOM) prevents more than 90\% of the oceanic methane emission to the atmosphere. In a previous study, we demonstrated that the high methane pressure (1, 4.5, and 8 MPa) stimulated in vitro SR-AOM activity. However, the information on the effect of high-pressure on the microbial community structure and architecture was still lacking. 
Results: In this study we analysed the long-term enrichment (286 days) of this microbial community, which was mediating SR-AOM in a continuous high-pressure bioreactor. 99.7\% of the total biovolume represented cells in the form of small aggregates (diameter less then 15 mu m). An increase of the total biovolume was observed (2.5 times). After 286 days, the ANME-2 (anaerobic methanotrophic archaea subgroup 2) and SRB (sulphate reducing bacteria) increased with a factor 12.5 and 8.4, respectively. 
Conclusion: This paper reports a net biomass growth of communities involved in SR-AOM, incubated at high-pressure.},
  articleno    = {137},
  author       = {Zhang, Yu and Maignien, Lo{\"i}s and Zhao, Xianxian and Wang, Fengping and Boon, Nico},
  issn         = {1471-2180},
  journal      = {BMC MICROBIOLOGY},
  keyword      = {MARINE-SEDIMENTS,METHANOTROPHIC ARCHAEA,DIVERSITY,ABUNDANCE,GROWTH,IDENTIFICATION,MOSBY MUD VOLCANO,CONTINUOUS-FLOW BIOREACTOR,SULFATE-REDUCING BACTERIA,OXIDIZING ARCHAEA},
  language     = {eng},
  pages        = {8},
  title        = {Enrichment of a microbial community performing anaerobic oxidation of methane in a continuous high-pressure bioreactor},
  url          = {http://dx.doi.org/10.1186/1471-2180-11-137},
  volume       = {11},
  year         = {2011},
}

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