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Effects of sprint training combined with vegetarian or mixed diet on muscle carnosine content and buffering capacity

Audrey Baguet UGent, Inge Everaert UGent, Hélène De Naeyer UGent, Harmen Reyngoudt UGent, Sanne Stegen UGent, Sam Beeckman UGent, Eric Achten UGent, Lander Vanhee UGent, Anneke Volkaert UGent and Mirko Petrovic UGent, et al. (2011) EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY. 111(10). p.2571-2580
abstract
Carnosine is an abundant dipeptide in human skeletal muscle with proton buffering capacity. There is controversy as to whether training can increase muscle carnosine and thereby provide a mechanism for increased buffering capacity. This study investigated the effects of 5 weeks sprint training combined with a vegetarian or mixed diet on muscle carnosine, carnosine synthase mRNA expression and muscle buffering capacity. Twenty omnivorous subjects participated in a 5 week sprint training intervention (2-3 times per week). They were randomized into a vegetarian and mixed diet group. Measurements (before and after the intervention period) included carnosine content in soleus, gastrocnemius lateralis and tibialis anterior by proton magnetic resonance spectroscopy ((1)H-MRS), true-cut biopsy of the gastrocnemius lateralis to determine in vitro non-bicarbonate muscle buffering capacity, carnosine content (HPLC method) and carnosine synthase (CARNS) mRNA expression and 6 x 6 s repeated sprint ability (RSA) test. There was a significant diet x training interaction in soleus carnosine content, which was non-significantly increased (+11%) with mixed diet and non-significantly decreased (-9%) with vegetarian diet. Carnosine content in other muscles and gastrocnemius buffer capacity were not influenced by training. CARNS mRNA expression was independent of training, but decreased significantly in the vegetarian group. The performance during the RSA test improved by training, without difference between groups. We found a positive correlation (r = 0.517; p = 0.002) between an invasive and non-invasive method for muscle carnosine quantification. In conclusion, this study shows that 5 weeks sprint training has no effect on the muscle carnosine content and carnosine synthase mRNA.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Muscle buffering capacity, Muscle carnosine, Sprint training, Vegetarian diet, BETA-ALANINE SUPPLEMENTATION, HUMAN SKELETAL-MUSCLE, EXERCISE PERFORMANCE, INTENSITY, TRANSPORT, ENDURANCE, LACTATE, ADAPTATIONS, HUMANS, METABOLISM
journal title
EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY
Eur. J. Appl. Physiol.
volume
111
issue
10
pages
2571 - 2580
Web of Science type
Article
Web of Science id
000294961500017
JCR category
SPORT SCIENCES
JCR impact factor
2.147 (2011)
JCR rank
18/83 (2011)
JCR quartile
1 (2011)
ISSN
1439-6319
DOI
10.1007/s00421-011-1877-4
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1888030
handle
http://hdl.handle.net/1854/LU-1888030
date created
2011-08-11 23:26:35
date last changed
2015-06-17 09:49:37
@article{1888030,
  abstract     = {Carnosine is an abundant dipeptide in human skeletal muscle with proton buffering capacity. There is controversy as to whether training can increase muscle carnosine and thereby provide a mechanism for increased buffering capacity. This study investigated the effects of 5 weeks sprint training combined with a vegetarian or mixed diet on muscle carnosine, carnosine synthase mRNA expression and muscle buffering capacity. Twenty omnivorous subjects participated in a 5 week sprint training intervention (2-3 times per week). They were randomized into a vegetarian and mixed diet group. Measurements (before and after the intervention period) included carnosine content in soleus, gastrocnemius lateralis and tibialis anterior by proton magnetic resonance spectroscopy ((1)H-MRS), true-cut biopsy of the gastrocnemius lateralis to determine in vitro non-bicarbonate muscle buffering capacity, carnosine content (HPLC method) and carnosine synthase (CARNS) mRNA expression and 6 x 6 s repeated sprint ability (RSA) test. There was a significant diet x training interaction in soleus carnosine content, which was non-significantly increased (+11\%) with mixed diet and non-significantly decreased (-9\%) with vegetarian diet. Carnosine content in other muscles and gastrocnemius buffer capacity were not influenced by training. CARNS mRNA expression was independent of training, but decreased significantly in the vegetarian group. The performance during the RSA test improved by training, without difference between groups. We found a positive correlation (r = 0.517; p = 0.002) between an invasive and non-invasive method for muscle carnosine quantification. In conclusion, this study shows that 5 weeks sprint training has no effect on the muscle carnosine content and carnosine synthase mRNA.},
  author       = {Baguet, Audrey and Everaert, Inge and De Naeyer, H{\'e}l{\`e}ne and Reyngoudt, Harmen and Stegen, Sanne and Beeckman, Sam and Achten, Eric and Vanhee, Lander and Volkaert, Anneke and Petrovic, Mirko and Taes, Youri and Derave, Wim},
  issn         = {1439-6319},
  journal      = {EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY},
  keyword      = {Muscle buffering capacity,Muscle carnosine,Sprint training,Vegetarian diet,BETA-ALANINE SUPPLEMENTATION,HUMAN SKELETAL-MUSCLE,EXERCISE PERFORMANCE,INTENSITY,TRANSPORT,ENDURANCE,LACTATE,ADAPTATIONS,HUMANS,METABOLISM},
  language     = {eng},
  number       = {10},
  pages        = {2571--2580},
  title        = {Effects of sprint training combined with vegetarian or mixed diet on muscle carnosine content and buffering capacity},
  url          = {http://dx.doi.org/10.1007/s00421-011-1877-4},
  volume       = {111},
  year         = {2011},
}

Chicago
Baguet, Audrey, Inge Everaert, Hélène De Naeyer, Harmen Reyngoudt, Sanne Stegen, Sam Beeckman, Eric Achten, et al. 2011. “Effects of Sprint Training Combined with Vegetarian or Mixed Diet on Muscle Carnosine Content and Buffering Capacity.” European Journal of Applied Physiology 111 (10): 2571–2580.
APA
Baguet, A., Everaert, I., De Naeyer, H., Reyngoudt, H., Stegen, S., Beeckman, S., Achten, E., et al. (2011). Effects of sprint training combined with vegetarian or mixed diet on muscle carnosine content and buffering capacity. EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY, 111(10), 2571–2580.
Vancouver
1.
Baguet A, Everaert I, De Naeyer H, Reyngoudt H, Stegen S, Beeckman S, et al. Effects of sprint training combined with vegetarian or mixed diet on muscle carnosine content and buffering capacity. EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY. 2011;111(10):2571–80.
MLA
Baguet, Audrey, Inge Everaert, Hélène De Naeyer, et al. “Effects of Sprint Training Combined with Vegetarian or Mixed Diet on Muscle Carnosine Content and Buffering Capacity.” EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY 111.10 (2011): 2571–2580. Print.