Advanced search
1 file | 1.09 MB

Transgenic Arabidopsis tester lines with dominant marker genes

(1996) MOLECULAR AND GENERAL GENETICS. 251(3). p.365-372
Author
Organization
Abstract
The map positions of a set of eight T-DNA insertions in the Arabidopsis genome have been determined by using closely linked visible markers. The insertions are dispersed over four of the five chromosomes. Each T-DNA insert contains one or more of the chimeric marker genes neomycin phosphotransferase (neo), hygromycin phosphotransferase (hpt), phosphinothricin acetyltransferase (bal), beta-glucuronidase (gusA) and indole-3-acetamide hydrolase (iaaH). The neo, hpt and bar marker genes are dominant in a selective germination assay or when used as DNA markers in a polymerase chain reaction. These dominant markers will allow recombinants to be discerned in a germinating F2 population, one generation earlier than with a conventional recessive marker. The transgenic marker lines will speed up and simplify the isolation of recombinants in small genetic intervals, a rate-limiting step in positional cloning strategies. The transgenic lines containing the hpt marker will also be of interest for the isolation of deletion mutants at the T-DNA integration sites.
Keywords
MUTATIONS, CLONING, THALIANA, RESISTANCE, DNA, ELEMENT AC, PLANT-CELLS, RECOMBINANT INBRED LINES, POLYMORPHISM LINKAGE MAP, T-DNA localization, recombinant selection, map-based cloning, Arabidopsis thaliana, dominant markers, GENOME

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 1.09 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Van Lijsebettens, Maria, Xuan Wang, Gerda Cnops, Wout Boerjan, Thierry Desnos, Herman Höfte, and Marc Van Montagu. 1996. “Transgenic Arabidopsis Tester Lines with Dominant Marker Genes.” Molecular and General Genetics 251 (3): 365–372.
APA
Van Lijsebettens, M., Wang, X., Cnops, G., Boerjan, W., Desnos, T., Höfte, H., & Van Montagu, M. (1996). Transgenic Arabidopsis tester lines with dominant marker genes. MOLECULAR AND GENERAL GENETICS, 251(3), 365–372.
Vancouver
1.
Van Lijsebettens M, Wang X, Cnops G, Boerjan W, Desnos T, Höfte H, et al. Transgenic Arabidopsis tester lines with dominant marker genes. MOLECULAR AND GENERAL GENETICS. 1996;251(3):365–72.
MLA
Van Lijsebettens, Maria, Xuan Wang, Gerda Cnops, et al. “Transgenic Arabidopsis Tester Lines with Dominant Marker Genes.” MOLECULAR AND GENERAL GENETICS 251.3 (1996): 365–372. Print.
@article{188744,
  abstract     = {The map positions of a set of eight T-DNA insertions in the Arabidopsis genome have been determined by using closely linked visible markers. The insertions are dispersed over four of the five chromosomes. Each T-DNA insert contains one or more of the chimeric marker genes neomycin phosphotransferase (neo), hygromycin phosphotransferase (hpt), phosphinothricin acetyltransferase (bal), beta-glucuronidase (gusA) and indole-3-acetamide hydrolase (iaaH). The neo, hpt and bar marker genes are dominant in a selective germination assay or when used as DNA markers in a polymerase chain reaction. These dominant markers will allow recombinants to be discerned in a germinating F2 population, one generation earlier than with a conventional recessive marker. The transgenic marker lines will speed up and simplify the isolation of recombinants in small genetic intervals, a rate-limiting step in positional cloning strategies. The transgenic lines containing the hpt marker will also be of interest for the isolation of deletion mutants at the T-DNA integration sites.},
  author       = {Van Lijsebettens, Maria and Wang, Xuan and Cnops, Gerda and Boerjan, Wout and Desnos, Thierry and H{\"o}fte, Herman and Van Montagu, Marc},
  issn         = {0026-8925},
  journal      = {MOLECULAR AND GENERAL GENETICS},
  keyword      = {MUTATIONS,CLONING,THALIANA,RESISTANCE,DNA,ELEMENT AC,PLANT-CELLS,RECOMBINANT INBRED LINES,POLYMORPHISM LINKAGE MAP,T-DNA localization,recombinant selection,map-based cloning,Arabidopsis thaliana,dominant markers,GENOME},
  language     = {eng},
  number       = {3},
  pages        = {365--372},
  title        = {Transgenic Arabidopsis tester lines with dominant marker genes},
  url          = {http://dx.doi.org/10.1007/BF02172528},
  volume       = {251},
  year         = {1996},
}

Altmetric
View in Altmetric