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Transgenic Arabidopsis tester lines with dominant marker genes

Maria Van Lijsebettens UGent, Xuan Wang UGent, Gerda Cnops UGent, Wout Boerjan UGent, Thierry Desnos, Herman Höfte and Marc Van Montagu UGent (1996) MOLECULAR AND GENERAL GENETICS. 251(3). p.365-372
abstract
The map positions of a set of eight T-DNA insertions in the Arabidopsis genome have been determined by using closely linked visible markers. The insertions are dispersed over four of the five chromosomes. Each T-DNA insert contains one or more of the chimeric marker genes neomycin phosphotransferase (neo), hygromycin phosphotransferase (hpt), phosphinothricin acetyltransferase (bal), beta-glucuronidase (gusA) and indole-3-acetamide hydrolase (iaaH). The neo, hpt and bar marker genes are dominant in a selective germination assay or when used as DNA markers in a polymerase chain reaction. These dominant markers will allow recombinants to be discerned in a germinating F2 population, one generation earlier than with a conventional recessive marker. The transgenic marker lines will speed up and simplify the isolation of recombinants in small genetic intervals, a rate-limiting step in positional cloning strategies. The transgenic lines containing the hpt marker will also be of interest for the isolation of deletion mutants at the T-DNA integration sites.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
MUTATIONS, CLONING, THALIANA, RESISTANCE, DNA, ELEMENT AC, PLANT-CELLS, RECOMBINANT INBRED LINES, POLYMORPHISM LINKAGE MAP, T-DNA localization, recombinant selection, map-based cloning, Arabidopsis thaliana, dominant markers, GENOME
journal title
MOLECULAR AND GENERAL GENETICS
Mol. Gen. Genet.
volume
251
issue
3
pages
365 - 372
Web of Science type
Article
ISSN
0026-8925
DOI
10.1007/BF02172528
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
188744
handle
http://hdl.handle.net/1854/LU-188744
date created
2004-01-14 13:41:00
date last changed
2013-10-30 14:56:42
@article{188744,
  abstract     = {The map positions of a set of eight T-DNA insertions in the Arabidopsis genome have been determined by using closely linked visible markers. The insertions are dispersed over four of the five chromosomes. Each T-DNA insert contains one or more of the chimeric marker genes neomycin phosphotransferase (neo), hygromycin phosphotransferase (hpt), phosphinothricin acetyltransferase (bal), beta-glucuronidase (gusA) and indole-3-acetamide hydrolase (iaaH). The neo, hpt and bar marker genes are dominant in a selective germination assay or when used as DNA markers in a polymerase chain reaction. These dominant markers will allow recombinants to be discerned in a germinating F2 population, one generation earlier than with a conventional recessive marker. The transgenic marker lines will speed up and simplify the isolation of recombinants in small genetic intervals, a rate-limiting step in positional cloning strategies. The transgenic lines containing the hpt marker will also be of interest for the isolation of deletion mutants at the T-DNA integration sites.},
  author       = {Van Lijsebettens, Maria and Wang, Xuan and Cnops, Gerda and Boerjan, Wout and Desnos, Thierry and H{\"o}fte, Herman and Van Montagu, Marc},
  issn         = {0026-8925},
  journal      = {MOLECULAR AND GENERAL GENETICS},
  keyword      = {MUTATIONS,CLONING,THALIANA,RESISTANCE,DNA,ELEMENT AC,PLANT-CELLS,RECOMBINANT INBRED LINES,POLYMORPHISM LINKAGE MAP,T-DNA localization,recombinant selection,map-based cloning,Arabidopsis thaliana,dominant markers,GENOME},
  language     = {eng},
  number       = {3},
  pages        = {365--372},
  title        = {Transgenic Arabidopsis tester lines with dominant marker genes},
  url          = {http://dx.doi.org/10.1007/BF02172528},
  volume       = {251},
  year         = {1996},
}

Chicago
Van Lijsebettens, Maria, Xuan Wang, Gerda Cnops, Wout Boerjan, Thierry Desnos, Herman Höfte, and Marc Van Montagu. 1996. “Transgenic Arabidopsis Tester Lines with Dominant Marker Genes.” Molecular and General Genetics 251 (3): 365–372.
APA
Van Lijsebettens, M., Wang, X., Cnops, G., Boerjan, W., Desnos, T., Höfte, H., & Van Montagu, M. (1996). Transgenic Arabidopsis tester lines with dominant marker genes. MOLECULAR AND GENERAL GENETICS, 251(3), 365–372.
Vancouver
1.
Van Lijsebettens M, Wang X, Cnops G, Boerjan W, Desnos T, Höfte H, et al. Transgenic Arabidopsis tester lines with dominant marker genes. MOLECULAR AND GENERAL GENETICS. 1996;251(3):365–72.
MLA
Van Lijsebettens, Maria, Xuan Wang, Gerda Cnops, et al. “Transgenic Arabidopsis Tester Lines with Dominant Marker Genes.” MOLECULAR AND GENERAL GENETICS 251.3 (1996): 365–372. Print.