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Role of nodI and nodJ in lipo-chitooligosaccharide secretion in Azorhizobium caulinodans and Escherichia coli

(1996) MOLECULAR MICROBIOLOGY. 20(5). p.993-1000
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Abstract
Lipo-chitooligosaccharide (LCO) Nod factors are produced and secreted by rhizobia and trigger nodule development in leguminous host plants. The products of the bacterial nodlJ genes are related to transporters of capsular polysaccharides and were proposed to be involved in LCO transport. We have studied nodlJ of Azorhizobium caulinodans ORS571 by analysis of cell-associated and secreted radioactively labelled Nod factors in wild-type ORS571, a nodJ mutant and a complemented strain. Secretion was strongly reduced in the nodJ mutant, and restored to wild-type levels after complementation. Constructs were made for expression of combinations of different nod genes in Escherichia coil DH5 alpha. The strain DH5 alpha (pUCNABCSU) synthesized LCOs, but they were only secreted when a plasmid containing both nodl and nodJ was supplied in trans. nodi or nodJ alone was not sufficient. In E. coil as well as in Azorhizobium, the nodlJ-encoded transporter showed a specificity for more hydrophilic LCOs.
Keywords
DNA, PROTEINS, STEM, IDENTIFICATION, NODULATION, GENE, GRAM-NEGATIVE BACTERIA, RHIZOBIUM-LEGUMINOSARUM, TRANSPORT-SYSTEMS, SESBANIA-ROSTRATA

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Citation

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Chicago
Fernández-López, Manuel, Wim D’Haeze, Peter Mergaert, Christa Verplancke, Jean-Claude Promé, Marc Van Montagu, and Marcella Holsters. 1996. “Role of nodI and nodJ in Lipo-chitooligosaccharide Secretion in Azorhizobium Caulinodans and Escherichia Coli.” Molecular Microbiology 20 (5): 993–1000.
APA
Fernández-López, M., D’Haeze, W., Mergaert, P., Verplancke, C., Promé, J.-C., Van Montagu, M., & Holsters, M. (1996). Role of nodI and nodJ in lipo-chitooligosaccharide secretion in Azorhizobium caulinodans and Escherichia coli. MOLECULAR MICROBIOLOGY, 20(5), 993–1000.
Vancouver
1.
Fernández-López M, D’Haeze W, Mergaert P, Verplancke C, Promé J-C, Van Montagu M, et al. Role of nodI and nodJ in lipo-chitooligosaccharide secretion in Azorhizobium caulinodans and Escherichia coli. MOLECULAR MICROBIOLOGY. 1996;20(5):993–1000.
MLA
Fernández-López, Manuel, Wim D’Haeze, Peter Mergaert, et al. “Role of nodI and nodJ in Lipo-chitooligosaccharide Secretion in Azorhizobium Caulinodans and Escherichia Coli.” MOLECULAR MICROBIOLOGY 20.5 (1996): 993–1000. Print.
@article{188596,
  abstract     = {Lipo-chitooligosaccharide (LCO) Nod factors are produced and secreted by rhizobia and trigger nodule development in leguminous host plants. The products of the bacterial nodlJ genes are related to transporters of capsular polysaccharides and were proposed to be involved in LCO transport. We have studied nodlJ of Azorhizobium caulinodans ORS571 by analysis of cell-associated and secreted radioactively labelled Nod factors in wild-type ORS571, a nodJ mutant and a complemented strain. Secretion was strongly reduced in the nodJ mutant, and restored to wild-type levels after complementation. Constructs were made for expression of combinations of different nod genes in Escherichia coil DH5 alpha. The strain DH5 alpha (pUCNABCSU) synthesized LCOs, but they were only secreted when a plasmid containing both nodl and nodJ was supplied in trans. nodi or nodJ alone was not sufficient. In E. coil as well as in Azorhizobium, the nodlJ-encoded transporter showed a specificity for more hydrophilic LCOs.},
  author       = {Fern{\'a}ndez-L{\'o}pez, Manuel and D'Haeze, Wim and Mergaert, Peter and Verplancke, Christa and Prom{\'e}, Jean-Claude and Van Montagu, Marc and Holsters, Marcella},
  issn         = {0950-382X},
  journal      = {MOLECULAR MICROBIOLOGY},
  keyword      = {DNA,PROTEINS,STEM,IDENTIFICATION,NODULATION,GENE,GRAM-NEGATIVE BACTERIA,RHIZOBIUM-LEGUMINOSARUM,TRANSPORT-SYSTEMS,SESBANIA-ROSTRATA},
  language     = {eng},
  number       = {5},
  pages        = {993--1000},
  title        = {Role of nodI and nodJ in lipo-chitooligosaccharide secretion in Azorhizobium caulinodans and Escherichia coli},
  url          = {http://dx.doi.org/10.1111/j.1365-2958.1996.tb02540.x},
  volume       = {20},
  year         = {1996},
}

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