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Role of nodI and nodJ in lipo-chitooligosaccharide secretion in Azorhizobium caulinodans and Escherichia coli

Manuel Fernández-López, Wim D'Haeze UGent, Peter Mergaert UGent, Christa Verplancke UGent, Jean-Claude Promé, Marc Van Montagu UGent and Marcella Holsters UGent (1996) MOLECULAR MICROBIOLOGY. 20(5). p.993-1000
abstract
Lipo-chitooligosaccharide (LCO) Nod factors are produced and secreted by rhizobia and trigger nodule development in leguminous host plants. The products of the bacterial nodlJ genes are related to transporters of capsular polysaccharides and were proposed to be involved in LCO transport. We have studied nodlJ of Azorhizobium caulinodans ORS571 by analysis of cell-associated and secreted radioactively labelled Nod factors in wild-type ORS571, a nodJ mutant and a complemented strain. Secretion was strongly reduced in the nodJ mutant, and restored to wild-type levels after complementation. Constructs were made for expression of combinations of different nod genes in Escherichia coil DH5 alpha. The strain DH5 alpha (pUCNABCSU) synthesized LCOs, but they were only secreted when a plasmid containing both nodl and nodJ was supplied in trans. nodi or nodJ alone was not sufficient. In E. coil as well as in Azorhizobium, the nodlJ-encoded transporter showed a specificity for more hydrophilic LCOs.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
DNA, PROTEINS, STEM, IDENTIFICATION, NODULATION, GENE, GRAM-NEGATIVE BACTERIA, RHIZOBIUM-LEGUMINOSARUM, TRANSPORT-SYSTEMS, SESBANIA-ROSTRATA
journal title
MOLECULAR MICROBIOLOGY
Mol. Microbiol.
volume
20
issue
5
pages
993 - 1000
Web of Science type
Article
ISSN
0950-382X
DOI
10.1111/j.1365-2958.1996.tb02540.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
188596
handle
http://hdl.handle.net/1854/LU-188596
date created
2004-01-14 13:41:00
date last changed
2013-10-29 14:14:20
@article{188596,
  abstract     = {Lipo-chitooligosaccharide (LCO) Nod factors are produced and secreted by rhizobia and trigger nodule development in leguminous host plants. The products of the bacterial nodlJ genes are related to transporters of capsular polysaccharides and were proposed to be involved in LCO transport. We have studied nodlJ of Azorhizobium caulinodans ORS571 by analysis of cell-associated and secreted radioactively labelled Nod factors in wild-type ORS571, a nodJ mutant and a complemented strain. Secretion was strongly reduced in the nodJ mutant, and restored to wild-type levels after complementation. Constructs were made for expression of combinations of different nod genes in Escherichia coil DH5 alpha. The strain DH5 alpha (pUCNABCSU) synthesized LCOs, but they were only secreted when a plasmid containing both nodl and nodJ was supplied in trans. nodi or nodJ alone was not sufficient. In E. coil as well as in Azorhizobium, the nodlJ-encoded transporter showed a specificity for more hydrophilic LCOs.},
  author       = {Fern{\'a}ndez-L{\'o}pez, Manuel and D'Haeze, Wim and Mergaert, Peter and Verplancke, Christa and Prom{\'e}, Jean-Claude and Van Montagu, Marc and Holsters, Marcella},
  issn         = {0950-382X},
  journal      = {MOLECULAR MICROBIOLOGY},
  keyword      = {DNA,PROTEINS,STEM,IDENTIFICATION,NODULATION,GENE,GRAM-NEGATIVE BACTERIA,RHIZOBIUM-LEGUMINOSARUM,TRANSPORT-SYSTEMS,SESBANIA-ROSTRATA},
  language     = {eng},
  number       = {5},
  pages        = {993--1000},
  title        = {Role of nodI and nodJ in lipo-chitooligosaccharide secretion in Azorhizobium caulinodans and Escherichia coli},
  url          = {http://dx.doi.org/10.1111/j.1365-2958.1996.tb02540.x},
  volume       = {20},
  year         = {1996},
}

Chicago
Fernández-López, Manuel, Wim D’Haeze, Peter Mergaert, Christa Verplancke, Jean-Claude Promé, Marc Van Montagu, and Marcella Holsters. 1996. “Role of nodI and nodJ in Lipo-chitooligosaccharide Secretion in Azorhizobium Caulinodans and Escherichia Coli.” Molecular Microbiology 20 (5): 993–1000.
APA
Fernández-López, M., D’Haeze, W., Mergaert, P., Verplancke, C., Promé, J.-C., Van Montagu, M., & Holsters, M. (1996). Role of nodI and nodJ in lipo-chitooligosaccharide secretion in Azorhizobium caulinodans and Escherichia coli. MOLECULAR MICROBIOLOGY, 20(5), 993–1000.
Vancouver
1.
Fernández-López M, D’Haeze W, Mergaert P, Verplancke C, Promé J-C, Van Montagu M, et al. Role of nodI and nodJ in lipo-chitooligosaccharide secretion in Azorhizobium caulinodans and Escherichia coli. MOLECULAR MICROBIOLOGY. 1996;20(5):993–1000.
MLA
Fernández-López, Manuel, Wim D’Haeze, Peter Mergaert, et al. “Role of nodI and nodJ in Lipo-chitooligosaccharide Secretion in Azorhizobium Caulinodans and Escherichia Coli.” MOLECULAR MICROBIOLOGY 20.5 (1996): 993–1000. Print.