Localization of T-DNA insertions in petunia by fluorescence in situ hybridization: physical evidence for suppression of recombination
- Author
- Rogier ten Hoopen, Tim P Robbins, Paul F Fransz, Bob M Montijn, Oof Oud, Tom Gerats (UGent) and Nanne Nanninga
- Organization
- Abstract
- Using fluorescence in situ hybridization (FISH) with metaphase preparations, we localized a 4-kb single-copy T-DNA sequence in a group of petunia transformants, The selected T-DNAs previously had been shown to be linked to the phenotypic marker Fl on chromosome II, Linkage analysis had revealed that recombination around the FI locus is suppressed in a wide cross relative to an inbred recombination assay, The localization of six FI-linked T-DNAs and the Fl locus itself, using FISH, revealed a number of aspects of recombination in petunia: (1) the central region of chromosome II showed at least a 10-fold suppression of recombination in wide crosses relative to the distal region; (2) recombination in wide hybrids over two-thirds of the chromosome was extremely low; and (3) recombination between completely homologous chromosomes in an inbred cross also was suppressed in the central region, In addition, the T-DNAs were not evenly distributed along the chromosome, suggesting a possible preference for a distal position for T-DNA integration, Implications for such a preference are discussed.
- Keywords
- INTEGRATION, HYBRIDA, PLANTS
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-188436
- MLA
- ten Hoopen, Rogier, et al. “Localization of T-DNA Insertions in Petunia by Fluorescence in Situ Hybridization: Physical Evidence for Suppression of Recombination.” PLANT CELL, vol. 8, no. 5, 1996, pp. 823–30, doi:10.1105/tpc.8.5.823.
- APA
- ten Hoopen, R., Robbins, T. P., Fransz, P. F., Montijn, B. M., Oud, O., Gerats, T., & Nanninga, N. (1996). Localization of T-DNA insertions in petunia by fluorescence in situ hybridization: physical evidence for suppression of recombination. PLANT CELL, 8(5), 823–830. https://doi.org/10.1105/tpc.8.5.823
- Chicago author-date
- Hoopen, Rogier ten, Tim P Robbins, Paul F Fransz, Bob M Montijn, Oof Oud, Tom Gerats, and Nanne Nanninga. 1996. “Localization of T-DNA Insertions in Petunia by Fluorescence in Situ Hybridization: Physical Evidence for Suppression of Recombination.” PLANT CELL 8 (5): 823–30. https://doi.org/10.1105/tpc.8.5.823.
- Chicago author-date (all authors)
- ten Hoopen, Rogier, Tim P Robbins, Paul F Fransz, Bob M Montijn, Oof Oud, Tom Gerats, and Nanne Nanninga. 1996. “Localization of T-DNA Insertions in Petunia by Fluorescence in Situ Hybridization: Physical Evidence for Suppression of Recombination.” PLANT CELL 8 (5): 823–830. doi:10.1105/tpc.8.5.823.
- Vancouver
- 1.ten Hoopen R, Robbins TP, Fransz PF, Montijn BM, Oud O, Gerats T, et al. Localization of T-DNA insertions in petunia by fluorescence in situ hybridization: physical evidence for suppression of recombination. PLANT CELL. 1996;8(5):823–30.
- IEEE
- [1]R. ten Hoopen et al., “Localization of T-DNA insertions in petunia by fluorescence in situ hybridization: physical evidence for suppression of recombination,” PLANT CELL, vol. 8, no. 5, pp. 823–830, 1996.
@article{188436,
abstract = {{Using fluorescence in situ hybridization (FISH) with metaphase preparations, we localized a 4-kb single-copy T-DNA sequence in a group of petunia transformants, The selected T-DNAs previously had been shown to be linked to the phenotypic marker Fl on chromosome II, Linkage analysis had revealed that recombination around the FI locus is suppressed in a wide cross relative to an inbred recombination assay, The localization of six FI-linked T-DNAs and the Fl locus itself, using FISH, revealed a number of aspects of recombination in petunia: (1) the central region of chromosome II showed at least a 10-fold suppression of recombination in wide crosses relative to the distal region; (2) recombination in wide hybrids over two-thirds of the chromosome was extremely low; and (3) recombination between completely homologous chromosomes in an inbred cross also was suppressed in the central region, In addition, the T-DNAs were not evenly distributed along the chromosome, suggesting a possible preference for a distal position for T-DNA integration, Implications for such a preference are discussed.}},
author = {{ten Hoopen, Rogier and Robbins, Tim P and Fransz, Paul F and Montijn, Bob M and Oud, Oof and Gerats, Tom and Nanninga, Nanne}},
issn = {{1040-4651}},
journal = {{PLANT CELL}},
keywords = {{INTEGRATION,HYBRIDA,PLANTS}},
language = {{eng}},
number = {{5}},
pages = {{823--830}},
title = {{Localization of T-DNA insertions in petunia by fluorescence in situ hybridization: physical evidence for suppression of recombination}},
url = {{http://doi.org/10.1105/tpc.8.5.823}},
volume = {{8}},
year = {{1996}},
}
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