Ghent University Academic Bibliography

Advanced

An in vitro model for chick embryonic notochords

E Ghanem, Ludwine Messiaen and Leo De Ridder (1995) CYTOTECHNOLOGY. 18(3). p.227-233
abstract
A two step method to obtain mesenchymal free 3.5 day old chick embryonic notochords in vitro is presented. 1.) Notochords are isolated by mechanical microdissection from the embryos below the head and above the leg-buds. 2.) The dissected notochords are trypsinized to eliminate contaminating mesenchymal cells, while the perinotochordal sheath (PNS) is retained. After isolation and trypsinization, notochords are cut in standard 8mm lengths, explanted in vitro and incubated at 37 degrees C. Immediately before incubation and after 3 and 6 days in vitro, notochords are fixed and stained to follow the morphological changes. The total DNA content of notochords is measured before and during maintenance in vitro to evaluate their metabolic activities. Results show that during the in vitro period, the isolated mesenchymal free notochordal fragments can conserve their characteristic architecture. The total DNA content measurements indicate proliferative activity and a high viability of the notochords in our in vitro system. In the present study, an isolation and in vitro method is offered which might be an effective tool to study the metabolic activities of chick embryonic notochords in vitro in comparison to in vivo behaviour, in order to study the underlying mechanism of notochord regression.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
notochord, DNA content, in vitro, PERINOTOCHORDAL MATERIALS, SOMITE CHONDROGENESIS, COLLAGEN, INVITRO
journal title
CYTOTECHNOLOGY
Cytotechnology
volume
18
issue
3
pages
227 - 233
Web of Science type
Article
ISSN
0920-9069
DOI
10.1007/BF00767770
language
English
UGent publication?
yes
classification
A1
id
187613
handle
http://hdl.handle.net/1854/LU-187613
date created
2004-01-14 13:41:00
date last changed
2017-08-02 13:14:06
@article{187613,
  abstract     = {A two step method to obtain mesenchymal free 3.5 day old chick embryonic notochords in vitro is presented. 1.) Notochords are isolated by mechanical microdissection from the embryos below the head and above the leg-buds. 2.) The dissected notochords are trypsinized to eliminate contaminating mesenchymal cells, while the perinotochordal sheath (PNS) is retained. After isolation and trypsinization, notochords are cut in standard 8mm lengths, explanted in vitro and incubated at 37 degrees C. Immediately before incubation and after 3 and 6 days in vitro, notochords are fixed and stained to follow the morphological changes. The total DNA content of notochords is measured before and during maintenance in vitro to evaluate their metabolic activities. Results show that during the in vitro period, the isolated mesenchymal free notochordal fragments can conserve their characteristic architecture. The total DNA content measurements indicate proliferative activity and a high viability of the notochords in our in vitro system. In the present study, an isolation and in vitro method is offered which might be an effective tool to study the metabolic activities of chick embryonic notochords in vitro in comparison to in vivo behaviour, in order to study the underlying mechanism of notochord regression.},
  author       = {Ghanem, E and Messiaen, Ludwine and De Ridder, Leo},
  issn         = {0920-9069},
  journal      = {CYTOTECHNOLOGY},
  keyword      = {notochord,DNA content,in vitro,PERINOTOCHORDAL MATERIALS,SOMITE CHONDROGENESIS,COLLAGEN,INVITRO},
  language     = {eng},
  number       = {3},
  pages        = {227--233},
  title        = {An in vitro model for chick embryonic notochords},
  url          = {http://dx.doi.org/10.1007/BF00767770},
  volume       = {18},
  year         = {1995},
}

Chicago
Ghanem, E, Ludwine Messiaen, and Leo De Ridder. 1995. “An in Vitro Model for Chick Embryonic Notochords.” Cytotechnology 18 (3): 227–233.
APA
Ghanem, E., Messiaen, L., & De Ridder, L. (1995). An in vitro model for chick embryonic notochords. CYTOTECHNOLOGY, 18(3), 227–233.
Vancouver
1.
Ghanem E, Messiaen L, De Ridder L. An in vitro model for chick embryonic notochords. CYTOTECHNOLOGY. 1995;18(3):227–33.
MLA
Ghanem, E, Ludwine Messiaen, and Leo De Ridder. “An in Vitro Model for Chick Embryonic Notochords.” CYTOTECHNOLOGY 18.3 (1995): 227–233. Print.