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Tissue-specific Salmonella Typhimurium gene expression during persistence in pigs

Alexander Van Parys UGent, Filip Boyen UGent, Bregje Leyman UGent, Elin Verbrugghe UGent, Freddy Haesebrouck UGent and Frank Pasmans UGent (2011) PLOS ONE. 6(8).
abstract
Salmonellosis caused by Salmonella Typhimurium is one of the most important bacterial zoonotic diseases. The bacterium persists in pigs resulting in asymptomatic 'carrier pigs', generating a major source for Salmonella contamination of pork. Until now, very little is known concerning the mechanisms used by Salmonella Typhimurium during persistence in pigs. Using in vivo expression technology (IVET), a promoter-trap method based on Delta purA attenuation of the parent strain, we identified 37 Salmonella Typhimurium genes that were expressed 3 weeks post oral inoculation in the tonsils, ileum and ileocaecal lymph nodes of pigs. Several genes were expressed in all three analyzed organs, while other genes were only expressed in one or two organs. Subsequently, the identified IVET transformants were pooled and reintroduced in pigs to detect tissue-specific gene expression patterns. We found that efp and rpoZ were specifically expressed in the ileocaecal lymph nodes during Salmonella peristence in pigs. Furthermore, we compared the persistence ability of substitution mutants for the IVET-identified genes sifB and STM4067 to that of the wild type in a mixed infection model. The Delta STM4067::kanR was significantly attenuated in the ileum contents, caecum and caecum contents and faeces of pigs 3 weeks post inoculation, while deletion of the SPI-2 effector gene sifB did not affect Salmonella Typhimurium persistence. Although our list of identified genes is not exhaustive, we found that efp and rpoZ were specifically expressed in the ileocaecal lymph nodes of pigs and we identified STM4067 as a factor involved in Salmonella persistence in pigs. To our knowledge, our study is the first to identify Salmonella Typhimurium genes expressed during persistence in pigs.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
In vivo expression technology, rpoZ, Salmonella Typhimurium, persistence, pig, efp
journal title
PLOS ONE
PLoS One
volume
6
issue
8
article number
e24120
Web of Science type
Article
Web of Science id
000295832000094
JCR category
BIOLOGY
JCR impact factor
4.092 (2011)
JCR rank
12/84 (2011)
JCR quartile
1 (2011)
ISSN
1932-6203
DOI
10.1371/journal.pone.0024120
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
1872344
handle
http://hdl.handle.net/1854/LU-1872344
date created
2011-08-09 09:59:56
date last changed
2016-12-21 15:42:40
@article{1872344,
  abstract     = {Salmonellosis caused by Salmonella Typhimurium is one of the most important bacterial zoonotic diseases. The bacterium persists in pigs resulting in asymptomatic 'carrier pigs', generating a major source for Salmonella contamination of pork. Until now, very little is known concerning the mechanisms used by Salmonella Typhimurium during persistence in pigs. Using in vivo expression technology (IVET), a promoter-trap method based on Delta purA attenuation of the parent strain, we identified 37 Salmonella Typhimurium genes that were expressed 3 weeks post oral inoculation in the tonsils, ileum and ileocaecal lymph nodes of pigs. Several genes were expressed in all three analyzed organs, while other genes were only expressed in one or two organs. Subsequently, the identified IVET transformants were pooled and reintroduced in pigs to detect tissue-specific gene expression patterns. We found that efp and rpoZ were specifically expressed in the ileocaecal lymph nodes during Salmonella peristence in pigs. Furthermore, we compared the persistence ability of substitution mutants for the IVET-identified genes sifB and STM4067 to that of the wild type in a mixed infection model. The Delta STM4067::kanR was significantly attenuated in the ileum contents, caecum and caecum contents and faeces of pigs 3 weeks post inoculation, while deletion of the SPI-2 effector gene sifB did not affect Salmonella Typhimurium persistence. Although our list of identified genes is not exhaustive, we found that efp and rpoZ were specifically expressed in the ileocaecal lymph nodes of pigs and we identified STM4067 as a factor involved in Salmonella persistence in pigs. To our knowledge, our study is the first to identify Salmonella Typhimurium genes expressed during persistence in pigs.},
  articleno    = {e24120},
  author       = {Van Parys, Alexander and Boyen, Filip and Leyman, Bregje and Verbrugghe, Elin and Haesebrouck, Freddy and Pasmans, Frank},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keyword      = {In vivo expression technology,rpoZ,Salmonella Typhimurium,persistence,pig,efp},
  language     = {eng},
  number       = {8},
  title        = {Tissue-specific Salmonella Typhimurium gene expression during persistence in pigs},
  url          = {http://dx.doi.org/10.1371/journal.pone.0024120},
  volume       = {6},
  year         = {2011},
}

Chicago
Van Parys, Alexander, Filip Boyen, Bregje Leyman, Elin Verbrugghe, Freddy Haesebrouck, and Frank Pasmans. 2011. “Tissue-specific Salmonella Typhimurium Gene Expression During Persistence in Pigs.” Plos One 6 (8).
APA
Van Parys, A., Boyen, F., Leyman, B., Verbrugghe, E., Haesebrouck, F., & Pasmans, F. (2011). Tissue-specific Salmonella Typhimurium gene expression during persistence in pigs. PLOS ONE, 6(8).
Vancouver
1.
Van Parys A, Boyen F, Leyman B, Verbrugghe E, Haesebrouck F, Pasmans F. Tissue-specific Salmonella Typhimurium gene expression during persistence in pigs. PLOS ONE. 2011;6(8).
MLA
Van Parys, Alexander, Filip Boyen, Bregje Leyman, et al. “Tissue-specific Salmonella Typhimurium Gene Expression During Persistence in Pigs.” PLOS ONE 6.8 (2011): n. pag. Print.