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Evaluation of β-glucan particles as mucosal delivery system in the Peyer's patch regions of the murine small intestine

Rebecca De Smet (UGent) , Tine Demoor (UGent) , STEPHANIE VERSCHUERE (UGent) , Marijke Dierendonck (UGent) , Bruno De Geest (UGent) and Claude Cuvelier (UGent)
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Abstract
Oral vaccination is essential to generate protective local immunity against intestinal pathogens. However, antigen delivery to the inductive sites for mucosal immunity (the small intestine Peyer’s patches, PP) has proven to be particularly challenging. We evaluated the potential of β-glucan microcapsules to deliver antigen transmucosally in the PP regions of the murine small intestine. β-glucan microparticles (3-5 µm) were prepared from Saccharomyces cerevisiae and loaded with Alexa Fluor 488-conjugated bovine serum albumin. Particles were administered to male C57BL/6 mice (8-10 weeks old) via intestinal loops at a particle concentration of 100*106/ml. After one hour of incubation, transmucosal particle transport and uptake in the PP was evaluated by flow cytometry, confocal microscopy and transmission electron microscopy. Using flow cytometry, we could not observe any particle uptake in the main antigen presenting cell population, the dendritic cells. Interestingly, flow-cytometric analysis indicated a modest but clear uptake of particles in the B-cell population. Confocal microscopic images showed yeast particles localized in the Follicle Associated Epithelium. Moreover, transmission electron microscopy demonstrated transcellular transport of yeast particles in M-cells. Our data suggest that M-cells, but not subepithelial dendritic cells, are crucial for the transmucosal transport of β-glucan particles from the intestinal lumen to the PP.
Keywords
Mucosal immunology, oral vaccination, mouse, peyer's patches, GALT

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Chicago
De Smet, Rebecca, Tine Demoor, STEPHANIE VERSCHUERE, Marijke Dierendonck, Bruno De Geest, and Claude Cuvelier. 2011. “Evaluation of Β-glucan Particles as Mucosal Delivery System in the Peyer’s Patch Regions of the Murine Small Intestine.” In Mucosal Immunology, 15th International Congress, Abstracts.
APA
De Smet, Rebecca, Demoor, T., VERSCHUERE, S., Dierendonck, M., De Geest, B., & Cuvelier, C. (2011). Evaluation of β-glucan particles as mucosal delivery system in the Peyer’s patch regions of the murine small intestine. Mucosal Immunology, 15th International congress, Abstracts. Presented at the 15th International congress of Mucosal Immunology (ICMI 2011).
Vancouver
1.
De Smet R, Demoor T, VERSCHUERE S, Dierendonck M, De Geest B, Cuvelier C. Evaluation of β-glucan particles as mucosal delivery system in the Peyer’s patch regions of the murine small intestine. Mucosal Immunology, 15th International congress, Abstracts. 2011.
MLA
De Smet, Rebecca, Tine Demoor, STEPHANIE VERSCHUERE, et al. “Evaluation of Β-glucan Particles as Mucosal Delivery System in the Peyer’s Patch Regions of the Murine Small Intestine.” Mucosal Immunology, 15th International Congress, Abstracts. 2011. Print.
@inproceedings{1869323,
  abstract     = {Oral vaccination is essential to generate protective local immunity against intestinal pathogens. However, antigen delivery to the inductive sites for mucosal immunity (the small intestine Peyer{\textquoteright}s patches, PP) has proven to be particularly challenging.
We evaluated the potential of \ensuremath{\beta}-glucan microcapsules to deliver antigen transmucosally in the PP regions of the murine small intestine.
\ensuremath{\beta}-glucan microparticles (3-5 {\textmu}m) were prepared from Saccharomyces cerevisiae and loaded with Alexa Fluor 488-conjugated bovine serum albumin. Particles were administered to male C57BL/6 mice (8-10 weeks old) via intestinal loops at a particle concentration of 100*106/ml.
After one hour of incubation, transmucosal particle transport and uptake in the PP was evaluated by flow cytometry, confocal microscopy and transmission electron microscopy. Using flow cytometry, we could not observe any particle uptake in the main antigen presenting cell population, the dendritic cells. Interestingly, flow-cytometric analysis indicated a modest but clear uptake of particles in the B-cell population. Confocal microscopic images showed yeast particles localized in the Follicle Associated Epithelium. Moreover, transmission electron microscopy demonstrated transcellular transport of yeast particles in M-cells.
Our data suggest that M-cells, but not subepithelial dendritic cells, are crucial for the transmucosal transport of \ensuremath{\beta}-glucan particles from the intestinal lumen to the PP.},
  author       = {De Smet, Rebecca and Demoor, Tine and VERSCHUERE, STEPHANIE and Dierendonck, Marijke and De Geest, Bruno and Cuvelier, Claude},
  booktitle    = {Mucosal Immunology, 15th International congress, Abstracts},
  keyword      = {Mucosal immunology,oral vaccination,mouse,peyer's patches,GALT},
  language     = {eng},
  location     = {Paris, France},
  title        = {Evaluation of \ensuremath{\beta}-glucan particles as mucosal delivery system in the Peyer's patch regions of the murine small intestine},
  year         = {2011},
}