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The human keratinocyte two-dimensional gel protein database (update 1995): mapping components of signal transduction pathways

Julio E Celis, Hanne Holm Rasmussen, Paul Gromov, Eydfinnur Olsen, Peder Madsen, Henrik Leffers, Bent Honoré, Kurt Dejgaard, Henrik Vorum, Dan Back Kristensen, et al. (1995) ELECTROPHORESIS. 16(12). p.2177-2240
abstract
The master two-dimensional (2-D) gel database of human keratinocytes currently lists 3154 cellular proteins (2224 isoelectric focusing, IEF; and 930 nonequilibrium pH gradient electrophoresis, NEPHGE), many of which correspond to post-translational modifications. 1082 polypeptides have been identified (protein name, organelle components, etc.) using a procedure or a combination of procedures that include (i) comigration with known human proteins, (ii) 2-D gel immunoblotting using specific antibodies, (iii) microsequencing of Coomassie Brilliant Blue stained proteins, (iv) mass spectrometry, (v) vaccinia virus expression of full length cDNAs, and (vi) in vitro transcription/translation of full-length cDNAs. This year, special emphasis has been given to the identification of signal transduction components by using 2-D gel immunoblotting of crude keratinocyte lysates in combination with enhanced chemoluminescence (ECL) detection. Identified proteins are listed both in alphabetical order and with increasing SSP number, together with their M(r), pI, cellular localization and credit to the investigator(s) that aided in the identification. Ultimately, the aim of the comprehensive database is to gather - through a systematic study of ekeratinocytes - qualitative and quantitative information on proteins and their genes that may allow us to identify abnormal patterns of gene expression and to pinpoint signaling pathways and components affected in various skin diseases, cancer included.
Please use this url to cite or link to this publication:
@article{186859,
  abstract     = {The master two-dimensional (2-D) gel database of human keratinocytes currently lists 3154 cellular proteins (2224 isoelectric focusing, IEF; and 930 nonequilibrium pH gradient electrophoresis, NEPHGE), many of which correspond to post-translational modifications. 1082 polypeptides have been identified (protein name, organelle components, etc.) using a procedure or a combination of procedures that include (i) comigration with known human proteins, (ii) 2-D gel immunoblotting using specific antibodies, (iii) microsequencing of Coomassie Brilliant Blue stained proteins, (iv) mass spectrometry, (v) vaccinia virus expression of full length cDNAs, and (vi) in vitro transcription/translation of full-length cDNAs. This year, special emphasis has been given to the identification of signal transduction components by using 2-D gel immunoblotting of crude keratinocyte lysates in combination with enhanced chemoluminescence (ECL) detection. Identified proteins are listed both in alphabetical order and with increasing SSP number, together with their M(r), pI, cellular localization and credit to the investigator(s) that aided in the identification. Ultimately, the aim of the comprehensive database is to gather - through a systematic study of ekeratinocytes - qualitative and quantitative information on proteins and their genes that may allow us to identify abnormal patterns of gene expression and to pinpoint signaling pathways and components affected in various skin diseases, cancer included.},
  author       = {Celis, Julio E and Rasmussen, Hanne Holm and Gromov, Paul and Olsen, Eydfinnur and Madsen, Peder and Leffers, Henrik and Honor{\'e}, Bent and Dejgaard, Kurt and Vorum, Henrik and Kristensen, Dan Back and {\O}stergaard, Morten and Hauns{\o}, Anders and Jensen, Niels Aagaard and Celis, Ariana and Basse, Bodil and Lauridsen, Jette B and Ratz, Gitte P and Andersen, Annette H and Walbum, Else and Kj{\ae}rgaard, Inge and Andersen, Inger and Puype, Magda and Van Damme, Jozef and Vandekerckhove, Jo{\"e}l},
  issn         = {0173-0835},
  journal      = {ELECTROPHORESIS},
  keyword      = {two-dimensional gel protein database,keratinocytes,protein identification,signal transduction components,global cell,regulation,DNA-SEQUENCE DATA,AMNION CELLS AMA,MOLECULAR-CLONING,POLYACRYLAMIDE GELS,INTEGRATED APPROACH,PSORIATIC SKIN,ELECTROPHORESIS,EXPRESSION,DIFFERENTIATION,PROLIFERATION},
  language     = {eng},
  number       = {12},
  pages        = {2177--2240},
  title        = {The human keratinocyte two-dimensional gel protein database (update 1995): mapping components of signal transduction pathways},
  url          = {http://dx.doi.org/10.1002/elps.11501601355},
  volume       = {16},
  year         = {1995},
}

Chicago
Celis, Julio E, Hanne Holm Rasmussen, Paul Gromov, Eydfinnur Olsen, Peder Madsen, Henrik Leffers, Bent Honoré, et al. 1995. “The Human Keratinocyte Two-dimensional Gel Protein Database (update 1995): Mapping Components of Signal Transduction Pathways.” Electrophoresis 16 (12): 2177–2240.
APA
Celis, J. E., Rasmussen, H. H., Gromov, P., Olsen, E., Madsen, P., Leffers, H., Honoré, B., et al. (1995). The human keratinocyte two-dimensional gel protein database (update 1995): mapping components of signal transduction pathways. ELECTROPHORESIS, 16(12), 2177–2240.
Vancouver
1.
Celis JE, Rasmussen HH, Gromov P, Olsen E, Madsen P, Leffers H, et al. The human keratinocyte two-dimensional gel protein database (update 1995): mapping components of signal transduction pathways. ELECTROPHORESIS. 1995;16(12):2177–240.
MLA
Celis, Julio E, Hanne Holm Rasmussen, Paul Gromov, et al. “The Human Keratinocyte Two-dimensional Gel Protein Database (update 1995): Mapping Components of Signal Transduction Pathways.” ELECTROPHORESIS 16.12 (1995): 2177–2240. Print.