Detection of cleavage of a prenisin-mimicking decapeptide by Lactococcus lactis subsp. lactis endoproteinase activity
- Author
- Hans Nelis (UGent) , Luc De Vuyst, K Goubert, Bart Devreese (UGent) , Jozef Van Beeumen (UGent) , J Raymackers, Erick Vandamme (UGent) and Andreas De Leenheer (UGent)
- Organization
- Abstract
- As part of ongoing studies in the biosynthesis of the lantibiotic nisin, we investigated the proteolytic cleavage of a synthetic Fmoc-labeled decapeptide mimicking a key amino acid sequence of the precursor prenisin in extracts of a Lactococcus lactis subsp. lactis strain. Reverse-phase high-performance liquid chromatography with photodiode army detection was used to trace and purify potential enzymatic conversion products. Of the three newly appearing chromatographic peaks, one was identified by means of electrospray mass spectrometry, amino acid analysis. and amino acid sequencing as an Fmoc-labeled hexapeptide derived from cleavage at the Arg(-1)-Ile(+1) bond. This assay will be useful to monitor the purification of the endoproteinase that reportedly cleaves prenisin at the same Arg(-1)-Ile(+1) site present in the model substrate.
- Keywords
- Lactococcus endoproteinase, lantibiotics, nisin
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Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-186819
- MLA
- Nelis, Hans, et al. “Detection of Cleavage of a Prenisin-Mimicking Decapeptide by Lactococcus Lactis Subsp. Lactis Endoproteinase Activity.” ENZYME AND MICROBIAL TECHNOLOGY, vol. 18, no. 1, 1996, pp. 52–58, doi:10.1016/0141-0229(96)00060-9.
- APA
- Nelis, H., De Vuyst, L., Goubert, K., Devreese, B., Van Beeumen, J., Raymackers, J., … De Leenheer, A. (1996). Detection of cleavage of a prenisin-mimicking decapeptide by Lactococcus lactis subsp. lactis endoproteinase activity. ENZYME AND MICROBIAL TECHNOLOGY, 18(1), 52–58. https://doi.org/10.1016/0141-0229(96)00060-9
- Chicago author-date
- Nelis, Hans, Luc De Vuyst, K Goubert, Bart Devreese, Jozef Van Beeumen, J Raymackers, Erick Vandamme, and Andreas De Leenheer. 1996. “Detection of Cleavage of a Prenisin-Mimicking Decapeptide by Lactococcus Lactis Subsp. Lactis Endoproteinase Activity.” ENZYME AND MICROBIAL TECHNOLOGY 18 (1): 52–58. https://doi.org/10.1016/0141-0229(96)00060-9.
- Chicago author-date (all authors)
- Nelis, Hans, Luc De Vuyst, K Goubert, Bart Devreese, Jozef Van Beeumen, J Raymackers, Erick Vandamme, and Andreas De Leenheer. 1996. “Detection of Cleavage of a Prenisin-Mimicking Decapeptide by Lactococcus Lactis Subsp. Lactis Endoproteinase Activity.” ENZYME AND MICROBIAL TECHNOLOGY 18 (1): 52–58. doi:10.1016/0141-0229(96)00060-9.
- Vancouver
- 1.Nelis H, De Vuyst L, Goubert K, Devreese B, Van Beeumen J, Raymackers J, et al. Detection of cleavage of a prenisin-mimicking decapeptide by Lactococcus lactis subsp. lactis endoproteinase activity. ENZYME AND MICROBIAL TECHNOLOGY. 1996;18(1):52–8.
- IEEE
- [1]H. Nelis et al., “Detection of cleavage of a prenisin-mimicking decapeptide by Lactococcus lactis subsp. lactis endoproteinase activity,” ENZYME AND MICROBIAL TECHNOLOGY, vol. 18, no. 1, pp. 52–58, 1996.
@article{186819,
abstract = {{As part of ongoing studies in the biosynthesis of the lantibiotic nisin, we investigated the proteolytic cleavage of a synthetic Fmoc-labeled decapeptide mimicking a key amino acid sequence of the precursor prenisin in extracts of a Lactococcus lactis subsp. lactis strain. Reverse-phase high-performance liquid chromatography with photodiode army detection was used to trace and purify potential enzymatic conversion products. Of the three newly appearing chromatographic peaks, one was identified by means of electrospray mass spectrometry, amino acid analysis. and amino acid sequencing as an Fmoc-labeled hexapeptide derived from cleavage at the Arg(-1)-Ile(+1) bond. This assay will be useful to monitor the purification of the endoproteinase that reportedly cleaves prenisin at the same Arg(-1)-Ile(+1) site present in the model substrate.}},
author = {{Nelis, Hans and De Vuyst, Luc and Goubert, K and Devreese, Bart and Van Beeumen, Jozef and Raymackers, J and Vandamme, Erick and De Leenheer, Andreas}},
issn = {{0141-0229}},
journal = {{ENZYME AND MICROBIAL TECHNOLOGY}},
keywords = {{Lactococcus endoproteinase,lantibiotics,nisin}},
language = {{eng}},
number = {{1}},
pages = {{52--58}},
title = {{Detection of cleavage of a prenisin-mimicking decapeptide by Lactococcus lactis subsp. lactis endoproteinase activity}},
url = {{http://doi.org/10.1016/0141-0229(96)00060-9}},
volume = {{18}},
year = {{1996}},
}
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