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Bacterial monocultures, propionate, butyrate and H(2)O(2) modulate the expression, secretion and structure of the fasting-induced adipose factor in gut epithelial cell lines

Charlotte Grootaert UGent, Tom Van de Wiele UGent, Ilse Van Roosbroeck UGent, Sam Possemiers UGent, Anne-Sophie Vercoutter-Edouart, Willy Verstraete UGent, Marc Bracke UGent and Barbara Vanhoecke UGent (2011) ENVIRONMENTAL MICROBIOLOGY. 13(7). p.1778-1789
abstract
Previous research showed that an intestinal microbial community represses the fasting-induced adipose factor (FIAF) in the gut epithelium, thereby increasing fat storage in the host. This study was designed to investigate the overall effect of different bacterial species and metabolites on FIAF in intestinal (Caco-2, HT-29 and HCT-116) and hepatic (HepG2) cancer cell lines. First, we showed that FIAF was present in different isoforms, and secreted as N-glycosylated proteins, exclusively at the basal side of the cell monolayer. Second, co-incubation of cell lines with bacterial monocultures and metabolites altered both FIAF production and isoform appearance. Propionate and/or butyrate treatment increased FIAF expression and cleavage in all tested cell lines. In contrast, different bacteria induced cell line-specific FIAF modulation. Clostridium perfringens induced FIAF isoform changes in Caco-2 cells. Enterococcus faecalis and Bacteroides thetaiotaomicron treatment resulted in cell line-specific FIAF increases, whereas Escherichia coli significantly decreased FIAF expression in HCT-116 cells. Treatment with H(2)O(2) and peroxide-producing E. faecalis strains induced FIAF isoform changes in Caco-2 cells. Since bacteria and bacterial metabolites alter both FIAF production and isoform appearance, further investigation may reveal an important role for bacteria in FIAF-regulated physiological processes, such as cell differentiation and fat metabolism.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
IOPOIETIN-LIKE PROTEIN-4, ACTIVATED-RECEPTOR-GAMMA, DIET-INDUCED OBESITY, COLON-CANCER CELLS, CHAIN FATTY-ACIDS, ENTEROCYTIC DIFFERENTIATION, ENTEROCOCCUS-FAECALIS, HYDROGEN-PEROXIDE, CARCINOMA-CELLS, TARGET GENE
journal title
ENVIRONMENTAL MICROBIOLOGY
Environ. Microbiol.
volume
13
issue
7
pages
1778 - 1789
Web of Science type
Article
Web of Science id
000292308500009
JCR category
MICROBIOLOGY
JCR impact factor
5.843 (2011)
JCR rank
14/111 (2011)
JCR quartile
1 (2011)
ISSN
1462-2912
DOI
10.1111/j.1462-2920.2011.02482.x
project
Biotechnology for a sustainable economy (Bio-Economy)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1866107
handle
http://hdl.handle.net/1854/LU-1866107
date created
2011-08-03 14:52:48
date last changed
2013-02-27 12:59:21
@article{1866107,
  abstract     = {Previous research showed that an intestinal microbial community represses the fasting-induced adipose factor (FIAF) in the gut epithelium, thereby increasing fat storage in the host. This study was designed to investigate the overall effect of different bacterial species and metabolites on FIAF in intestinal (Caco-2, HT-29 and HCT-116) and hepatic (HepG2) cancer cell lines. First, we showed that FIAF was present in different isoforms, and secreted as N-glycosylated proteins, exclusively at the basal side of the cell monolayer. Second, co-incubation of cell lines with bacterial monocultures and metabolites altered both FIAF production and isoform appearance. Propionate and/or butyrate treatment increased FIAF expression and cleavage in all tested cell lines. In contrast, different bacteria induced cell line-specific FIAF modulation. Clostridium perfringens induced FIAF isoform changes in Caco-2 cells. Enterococcus faecalis and Bacteroides thetaiotaomicron treatment resulted in cell line-specific FIAF increases, whereas Escherichia coli significantly decreased FIAF expression in HCT-116 cells. Treatment with H(2)O(2) and peroxide-producing E. faecalis strains induced FIAF isoform changes in Caco-2 cells. Since bacteria and bacterial metabolites alter both FIAF production and isoform appearance, further investigation may reveal an important role for bacteria in FIAF-regulated physiological processes, such as cell differentiation and fat metabolism.},
  author       = {Grootaert, Charlotte and Van de Wiele, Tom and Van Roosbroeck, Ilse and Possemiers, Sam and Vercoutter-Edouart, Anne-Sophie and Verstraete, Willy and Bracke, Marc and Vanhoecke, Barbara},
  issn         = {1462-2912},
  journal      = {ENVIRONMENTAL MICROBIOLOGY},
  keyword      = {IOPOIETIN-LIKE PROTEIN-4,ACTIVATED-RECEPTOR-GAMMA,DIET-INDUCED OBESITY,COLON-CANCER CELLS,CHAIN FATTY-ACIDS,ENTEROCYTIC DIFFERENTIATION,ENTEROCOCCUS-FAECALIS,HYDROGEN-PEROXIDE,CARCINOMA-CELLS,TARGET GENE},
  language     = {eng},
  number       = {7},
  pages        = {1778--1789},
  title        = {Bacterial monocultures, propionate, butyrate and H(2)O(2) modulate the expression, secretion and structure of the fasting-induced adipose factor in gut epithelial cell lines},
  url          = {http://dx.doi.org/10.1111/j.1462-2920.2011.02482.x},
  volume       = {13},
  year         = {2011},
}

Chicago
Grootaert, Charlotte, Tom Van de Wiele, Ilse Van Roosbroeck, Sam Possemiers, Anne-Sophie Vercoutter-Edouart, Willy Verstraete, Marc Bracke, and Barbara Vanhoecke. 2011. “Bacterial Monocultures, Propionate, Butyrate and H(2)O(2) Modulate the Expression, Secretion and Structure of the Fasting-induced Adipose Factor in Gut Epithelial Cell Lines.” Environmental Microbiology 13 (7): 1778–1789.
APA
Grootaert, C., Van de Wiele, T., Van Roosbroeck, I., Possemiers, S., Vercoutter-Edouart, A.-S., Verstraete, W., Bracke, M., et al. (2011). Bacterial monocultures, propionate, butyrate and H(2)O(2) modulate the expression, secretion and structure of the fasting-induced adipose factor in gut epithelial cell lines. ENVIRONMENTAL MICROBIOLOGY, 13(7), 1778–1789.
Vancouver
1.
Grootaert C, Van de Wiele T, Van Roosbroeck I, Possemiers S, Vercoutter-Edouart A-S, Verstraete W, et al. Bacterial monocultures, propionate, butyrate and H(2)O(2) modulate the expression, secretion and structure of the fasting-induced adipose factor in gut epithelial cell lines. ENVIRONMENTAL MICROBIOLOGY. 2011;13(7):1778–89.
MLA
Grootaert, Charlotte, Tom Van de Wiele, Ilse Van Roosbroeck, et al. “Bacterial Monocultures, Propionate, Butyrate and H(2)O(2) Modulate the Expression, Secretion and Structure of the Fasting-induced Adipose Factor in Gut Epithelial Cell Lines.” ENVIRONMENTAL MICROBIOLOGY 13.7 (2011): 1778–1789. Print.