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A functional turbidimetric method to determine C-reactive protein in horses

Tugirimana Pierrot Lundimu UGent, Dominique De Clercq UGent, ASTRID HOLDERBEKE UGent, Jozef Kint UGent, Liesbeth De Cooman UGent, Piet Deprez UGent and Joris Delanghe UGent (2011) JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION. 23(2). p.308-311
abstract
A turbidimetric method to determine serum C-reactive protein (CRP) concentration, based on soybean oil phosphocholine interaction, was performed on horse serum samples to evaluate its potential diagnostic value in veterinary medicine. Intralipid 20% in 0.1 M Tris-calcium buffer (pH 7.5) was added to horse serum. After 30 min of incubation at 37 degrees C, the CRP phosphocholine complexes were turbidimetrically, bichromatically (660 nm/700 nm) quantified on a commercial analyzer. Furthermore, comparison between CRP and other inflammatory markers, including white blood cell and neutrophil counts, was performed to evaluate the diagnostic value of both tests. Standardization of the assay was done using a commercial human CRP calibrator. The CRP measurements were performed on serum samples (296 patients and 34 controls). Reference values were found to be lower than 10 mg/l. The method was found to be linear between 1 and 400 mg/l. A moderate correlation was observed between CRP values and the relative neutrophil counts. Receiver-operating characteristics analysis demonstrated the area under the curve for CRP was 0.928, which was superior (P < 0.001) to the neutrophil count (0.804) and the leukocyte count (0.664) in detecting the presence of inflammation. This CRP assay showed reliable results as an acute phase test in horses, confirming its species-independent capability to detect CRP in various mammals, including horses.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ACUTE-PHASE PROTEINS, SERUM, phosphocholine, soybean oil, L-index, immunoturbidimetry, C-reactive protein, horses, ASSAY
journal title
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
J. Vet. Diagn. Invest.
volume
23
issue
2
pages
308 - 311
Web of Science type
Article
Web of Science id
000288838300017
JCR category
VETERINARY SCIENCES
JCR impact factor
1.214 (2011)
JCR rank
43/141 (2011)
JCR quartile
2 (2011)
ISSN
1040-6387
DOI
10.1177/104063871102300217
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1864899
handle
http://hdl.handle.net/1854/LU-1864899
date created
2011-08-02 11:10:58
date last changed
2012-01-09 14:11:26
@article{1864899,
  abstract     = {A turbidimetric method to determine serum C-reactive protein (CRP) concentration, based on soybean oil phosphocholine interaction, was performed on horse serum samples to evaluate its potential diagnostic value in veterinary medicine. Intralipid 20\% in 0.1 M Tris-calcium buffer (pH 7.5) was added to horse serum. After 30 min of incubation at 37 degrees C, the CRP phosphocholine complexes were turbidimetrically, bichromatically (660 nm/700 nm) quantified on a commercial analyzer. Furthermore, comparison between CRP and other inflammatory markers, including white blood cell and neutrophil counts, was performed to evaluate the diagnostic value of both tests. Standardization of the assay was done using a commercial human CRP calibrator. The CRP measurements were performed on serum samples (296 patients and 34 controls). Reference values were found to be lower than 10 mg/l. The method was found to be linear between 1 and 400 mg/l. A moderate correlation was observed between CRP values and the relative neutrophil counts. Receiver-operating characteristics analysis demonstrated the area under the curve for CRP was 0.928, which was superior (P {\textlangle} 0.001) to the neutrophil count (0.804) and the leukocyte count (0.664) in detecting the presence of inflammation. This CRP assay showed reliable results as an acute phase test in horses, confirming its species-independent capability to detect CRP in various mammals, including horses.},
  author       = {Lundimu, Tugirimana Pierrot and De Clercq, Dominique and HOLDERBEKE, ASTRID and Kint, Jozef and De Cooman, Liesbeth and Deprez, Piet and Delanghe, Joris},
  issn         = {1040-6387},
  journal      = {JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION},
  keyword      = {ACUTE-PHASE PROTEINS,SERUM,phosphocholine,soybean oil,L-index,immunoturbidimetry,C-reactive protein,horses,ASSAY},
  language     = {eng},
  number       = {2},
  pages        = {308--311},
  title        = {A functional turbidimetric method to determine C-reactive protein in horses},
  url          = {http://dx.doi.org/10.1177/104063871102300217},
  volume       = {23},
  year         = {2011},
}

Chicago
Lundimu, Tugirimana Pierrot, Dominique De Clercq, ASTRID HOLDERBEKE, Jozef Kint, LIESBETH DE COOMAN, Piet Deprez, and Joris Delanghe. 2011. “A Functional Turbidimetric Method to Determine C-reactive Protein in Horses.” Journal of Veterinary Diagnostic Investigation 23 (2): 308–311.
APA
Lundimu, T. P., De Clercq, D., HOLDERBEKE, A., Kint, J., DE COOMAN, L., Deprez, P., & Delanghe, J. (2011). A functional turbidimetric method to determine C-reactive protein in horses. JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 23(2), 308–311.
Vancouver
1.
Lundimu TP, De Clercq D, HOLDERBEKE A, Kint J, DE COOMAN L, Deprez P, et al. A functional turbidimetric method to determine C-reactive protein in horses. JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION. 2011;23(2):308–11.
MLA
Lundimu, Tugirimana Pierrot, Dominique De Clercq, ASTRID HOLDERBEKE, et al. “A Functional Turbidimetric Method to Determine C-reactive Protein in Horses.” JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION 23.2 (2011): 308–311. Print.