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A functional turbidimetric method to determine C-reactive protein in horses

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Abstract
A turbidimetric method to determine serum C-reactive protein (CRP) concentration, based on soybean oil phosphocholine interaction, was performed on horse serum samples to evaluate its potential diagnostic value in veterinary medicine. Intralipid 20% in 0.1 M Tris-calcium buffer (pH 7.5) was added to horse serum. After 30 min of incubation at 37 degrees C, the CRP phosphocholine complexes were turbidimetrically, bichromatically (660 nm/700 nm) quantified on a commercial analyzer. Furthermore, comparison between CRP and other inflammatory markers, including white blood cell and neutrophil counts, was performed to evaluate the diagnostic value of both tests. Standardization of the assay was done using a commercial human CRP calibrator. The CRP measurements were performed on serum samples (296 patients and 34 controls). Reference values were found to be lower than 10 mg/l. The method was found to be linear between 1 and 400 mg/l. A moderate correlation was observed between CRP values and the relative neutrophil counts. Receiver-operating characteristics analysis demonstrated the area under the curve for CRP was 0.928, which was superior (P < 0.001) to the neutrophil count (0.804) and the leukocyte count (0.664) in detecting the presence of inflammation. This CRP assay showed reliable results as an acute phase test in horses, confirming its species-independent capability to detect CRP in various mammals, including horses.
Keywords
ACUTE-PHASE PROTEINS, SERUM, phosphocholine, soybean oil, L-index, immunoturbidimetry, C-reactive protein, horses, ASSAY

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Chicago
Lundimu, Tugirimana Pierrot, Dominique De Clercq, ASTRID HOLDERBEKE, Jozef Kint, LIESBETH DE COOMAN, Piet Deprez, and Joris Delanghe. 2011. “A Functional Turbidimetric Method to Determine C-reactive Protein in Horses.” Journal of Veterinary Diagnostic Investigation 23 (2): 308–311.
APA
Lundimu, T. P., De Clercq, D., HOLDERBEKE, A., Kint, J., DE COOMAN, L., Deprez, P., & Delanghe, J. (2011). A functional turbidimetric method to determine C-reactive protein in horses. JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 23(2), 308–311.
Vancouver
1.
Lundimu TP, De Clercq D, HOLDERBEKE A, Kint J, DE COOMAN L, Deprez P, et al. A functional turbidimetric method to determine C-reactive protein in horses. JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION. 2011;23(2):308–11.
MLA
Lundimu, Tugirimana Pierrot, Dominique De Clercq, ASTRID HOLDERBEKE, et al. “A Functional Turbidimetric Method to Determine C-reactive Protein in Horses.” JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION 23.2 (2011): 308–311. Print.
@article{1864899,
  abstract     = {A turbidimetric method to determine serum C-reactive protein (CRP) concentration, based on soybean oil phosphocholine interaction, was performed on horse serum samples to evaluate its potential diagnostic value in veterinary medicine. Intralipid 20\% in 0.1 M Tris-calcium buffer (pH 7.5) was added to horse serum. After 30 min of incubation at 37 degrees C, the CRP phosphocholine complexes were turbidimetrically, bichromatically (660 nm/700 nm) quantified on a commercial analyzer. Furthermore, comparison between CRP and other inflammatory markers, including white blood cell and neutrophil counts, was performed to evaluate the diagnostic value of both tests. Standardization of the assay was done using a commercial human CRP calibrator. The CRP measurements were performed on serum samples (296 patients and 34 controls). Reference values were found to be lower than 10 mg/l. The method was found to be linear between 1 and 400 mg/l. A moderate correlation was observed between CRP values and the relative neutrophil counts. Receiver-operating characteristics analysis demonstrated the area under the curve for CRP was 0.928, which was superior (P {\textlangle} 0.001) to the neutrophil count (0.804) and the leukocyte count (0.664) in detecting the presence of inflammation. This CRP assay showed reliable results as an acute phase test in horses, confirming its species-independent capability to detect CRP in various mammals, including horses.},
  author       = {Lundimu, Tugirimana Pierrot and De Clercq, Dominique and HOLDERBEKE, ASTRID and Kint, Jozef and DE COOMAN, LIESBETH and Deprez, Piet and Delanghe, Joris},
  issn         = {1040-6387},
  journal      = {JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION},
  keyword      = {ACUTE-PHASE PROTEINS,SERUM,phosphocholine,soybean oil,L-index,immunoturbidimetry,C-reactive protein,horses,ASSAY},
  language     = {eng},
  number       = {2},
  pages        = {308--311},
  title        = {A functional turbidimetric method to determine C-reactive protein in horses},
  url          = {http://dx.doi.org/10.1177/104063871102300217},
  volume       = {23},
  year         = {2011},
}

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