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Bacterial and plant signal integration via D3-type cyclins enhances symptom development in the Arabidopsis-Rhodococcus fascians interaction

(2011) PLANT PHYSIOLOGY. 156(2). p.712-725
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Abstract
The phytopathogenic actinomycete Rhodococcus fascians drives its host to form a nutrient-rich niche by secreting a mixture of cytokinins that triggers plant cell division and shoot formation. The discrepancy between the relatively low amount of secreted cytokinins and the severe impact of R. fascians infection on plant development has puzzled researchers for a long time. Polyamine and transcript profiling of wild-type and cytokinin receptor mutant plants revealed that the bacterial cytokinins directly stimulated the biosynthesis of plant putrescine by activating arginine decarboxylase expression. Pharmacological experiments showed that the increased levels of putrescine contributed to the severity of the symptoms. Thus, putrescine functions as a secondary signal that impinges on the cytokinin-activated pathway, amplifying the hormone-induced changes that lead to the formation of a leafy gall. Exogenous putrescine and treatment with polyamine biosynthesis inhibitors combined with transcript and polyamine analyses of wild-type and mutant plants indicated that the direct target of both the bacterial cytokinins and plant putrescine was the expression of D3-type cyclins. Hence, the activated D-type cyclin/retinoblastoma/E2F transcription factor pathway integrates both external and internal hormonal signals, stimulating mitotic cell divisions and inducing pathological plant organogenesis.
Keywords
BIOSYNTHESIS, GROWTH, THALIANA, CYTOKININ, CELL-CYCLE, POLYAMINE METABOLISM, SPERMIDINE SYNTHASE, INSERTIONAL MUTAGENESIS, GENE-EXPRESSION, SEED DEVELOPMENT

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Chicago
Stes, Elisabeth, Stefania Biondi, Marcella Holsters, and Danny Vereecke. 2011. “Bacterial and Plant Signal Integration via D3-type Cyclins Enhances Symptom Development in the Arabidopsis-Rhodococcus Fascians Interaction.” Plant Physiology 156 (2): 712–725.
APA
Stes, E., Biondi, S., Holsters, M., & Vereecke, D. (2011). Bacterial and plant signal integration via D3-type cyclins enhances symptom development in the Arabidopsis-Rhodococcus fascians interaction. PLANT PHYSIOLOGY, 156(2), 712–725.
Vancouver
1.
Stes E, Biondi S, Holsters M, Vereecke D. Bacterial and plant signal integration via D3-type cyclins enhances symptom development in the Arabidopsis-Rhodococcus fascians interaction. PLANT PHYSIOLOGY. 2011;156(2):712–25.
MLA
Stes, Elisabeth, Stefania Biondi, Marcella Holsters, et al. “Bacterial and Plant Signal Integration via D3-type Cyclins Enhances Symptom Development in the Arabidopsis-Rhodococcus Fascians Interaction.” PLANT PHYSIOLOGY 156.2 (2011): 712–725. Print.
@article{1864589,
  abstract     = {The phytopathogenic actinomycete Rhodococcus fascians drives its host to form a nutrient-rich niche by secreting a mixture of cytokinins that triggers plant cell division and shoot formation. The discrepancy between the relatively low amount of secreted cytokinins and the severe impact of R. fascians infection on plant development has puzzled researchers for a long time. Polyamine and transcript profiling of wild-type and cytokinin receptor mutant plants revealed that the bacterial cytokinins directly stimulated the biosynthesis of plant putrescine by activating arginine decarboxylase expression. Pharmacological experiments showed that the increased levels of putrescine contributed to the severity of the symptoms. Thus, putrescine functions as a secondary signal that impinges on the cytokinin-activated pathway, amplifying the hormone-induced changes that lead to the formation of a leafy gall. Exogenous putrescine and treatment with polyamine biosynthesis inhibitors combined with transcript and polyamine analyses of wild-type and mutant plants indicated that the direct target of both the bacterial cytokinins and plant putrescine was the expression of D3-type cyclins. Hence, the activated D-type cyclin/retinoblastoma/E2F transcription factor pathway integrates both external and internal hormonal signals, stimulating mitotic cell divisions and inducing pathological plant organogenesis.},
  author       = {Stes, Elisabeth and Biondi, Stefania and Holsters, Marcella and Vereecke, Danny},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  keyword      = {BIOSYNTHESIS,GROWTH,THALIANA,CYTOKININ,CELL-CYCLE,POLYAMINE METABOLISM,SPERMIDINE SYNTHASE,INSERTIONAL MUTAGENESIS,GENE-EXPRESSION,SEED DEVELOPMENT},
  language     = {eng},
  number       = {2},
  pages        = {712--725},
  title        = {Bacterial and plant signal integration via D3-type cyclins enhances symptom development in the Arabidopsis-Rhodococcus fascians interaction},
  url          = {http://dx.doi.org/10.1104/pp.110.171561},
  volume       = {156},
  year         = {2011},
}

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