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Metabolite profiling of Triterpene Saponins in medicago truncatula hairy roots by liquid chromatography fourier transform Ion Cyclotron resonance mass spectrometry

Jacob Pollier UGent, Kris Morreel UGent, Danny Geelen UGent and Alain Goossens UGent (2011) JOURNAL OF NATURAL PRODUCTS. 74(6). p.1462-1476
abstract
Triterpenes are one of the largest classes of plant natural products, with an enormous variety in structure and bioactivities. Here, triterpene saponins from hairy roots of the model legume Medicago truncatula were profiled with reversed-phase liquid chromatography coupled to negative-ion electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (LC ESI FT-ICR MS). Owing to the accuracy of the FT-ICR MS, reliable molecular formulas of the detected compounds could be predicted, which, together with the generated MS" spectra, allowed the tentative identification of 79 different saponins, of which 61 had not been detected previously in M. truncatula. Upon collision-induced dissociation of saponins that contain a uronic acid residue in the sugar chain, fragment ions resulting from cross-ring cleavages of the uronic acid residues were observed. The identified saponins are glycosides of 10 different sapogenins, of which three were not detected before in M. truncatula. Zanhic acid glycosides, which are prevalent in the aerial parts of M. truncatula, were absent in the hairy root extract's. This metabolite compendium will facilitate future functional genomic studies of triterpene saponin biosynthesis in M. truncptula.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
FLAVONOIDS, GENE, BIOSYNTHESIS, ISOFLAVONOIDS, CULTURES, AGROBACTERIUM, AERIAL PARTS, TRANSGENIC PLANTS, MS
journal title
JOURNAL OF NATURAL PRODUCTS
J. Nat. Prod.
volume
74
issue
6
pages
1462 - 1476
Web of Science type
Article
Web of Science id
000291920600016
JCR category
PLANT SCIENCES
JCR impact factor
3.128 (2011)
JCR rank
27/189 (2011)
JCR quartile
1 (2011)
ISSN
0163-3864
DOI
10.1021/np200218r
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1864562
handle
http://hdl.handle.net/1854/LU-1864562
date created
2011-08-01 19:06:15
date last changed
2016-12-19 15:41:56
@article{1864562,
  abstract     = {Triterpenes are one of the largest classes of plant natural products, with an enormous variety in structure and bioactivities. Here, triterpene saponins from hairy roots of the model legume Medicago truncatula were profiled with reversed-phase liquid chromatography coupled to negative-ion electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (LC ESI FT-ICR MS). Owing to the accuracy of the FT-ICR MS, reliable molecular formulas of the detected compounds could be predicted, which, together with the generated MS{\textacutedbl} spectra, allowed the tentative identification of 79 different saponins, of which 61 had not been detected previously in M. truncatula. Upon collision-induced dissociation of saponins that contain a uronic acid residue in the sugar chain, fragment ions resulting from cross-ring cleavages of the uronic acid residues were observed. The identified saponins are glycosides of 10 different sapogenins, of which three were not detected before in M. truncatula. Zanhic acid glycosides, which are prevalent in the aerial parts of M. truncatula, were absent in the hairy root extract's. This metabolite compendium will facilitate future functional genomic studies of triterpene saponin biosynthesis in M. truncptula.},
  author       = {Pollier, Jacob and Morreel, Kris and Geelen, Danny and Goossens, Alain},
  issn         = {0163-3864},
  journal      = {JOURNAL OF NATURAL PRODUCTS},
  keyword      = {FLAVONOIDS,GENE,BIOSYNTHESIS,ISOFLAVONOIDS,CULTURES,AGROBACTERIUM,AERIAL PARTS,TRANSGENIC PLANTS,MS},
  language     = {eng},
  number       = {6},
  pages        = {1462--1476},
  title        = {Metabolite profiling of Triterpene Saponins in medicago truncatula hairy roots by liquid chromatography fourier transform Ion Cyclotron resonance mass spectrometry},
  url          = {http://dx.doi.org/10.1021/np200218r},
  volume       = {74},
  year         = {2011},
}

Chicago
Pollier, Jacob, Kris Morreel, Danny Geelen, and Alain Goossens. 2011. “Metabolite Profiling of Triterpene Saponins in Medicago Truncatula Hairy Roots by Liquid Chromatography Fourier Transform Ion Cyclotron Resonance Mass Spectrometry.” Journal of Natural Products 74 (6): 1462–1476.
APA
Pollier, J., Morreel, K., Geelen, D., & Goossens, A. (2011). Metabolite profiling of Triterpene Saponins in medicago truncatula hairy roots by liquid chromatography fourier transform Ion Cyclotron resonance mass spectrometry. JOURNAL OF NATURAL PRODUCTS, 74(6), 1462–1476.
Vancouver
1.
Pollier J, Morreel K, Geelen D, Goossens A. Metabolite profiling of Triterpene Saponins in medicago truncatula hairy roots by liquid chromatography fourier transform Ion Cyclotron resonance mass spectrometry. JOURNAL OF NATURAL PRODUCTS. 2011;74(6):1462–76.
MLA
Pollier, Jacob, Kris Morreel, Danny Geelen, et al. “Metabolite Profiling of Triterpene Saponins in Medicago Truncatula Hairy Roots by Liquid Chromatography Fourier Transform Ion Cyclotron Resonance Mass Spectrometry.” JOURNAL OF NATURAL PRODUCTS 74.6 (2011): 1462–1476. Print.