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Cell plate restricted association of DRP1A and PIN proteins is required for cell polarity establishment in Arabidopsis

Jozef Mravec (UGent) , Jan Petrasek, Na Li, Sjef Boeren, Rumyana Karlova, Saeko Kitakura (UGent) , Markéta Parezova, Satoshi Naramoto (UGent) , Tomasz Nodzyński (UGent) , Pankaj Dhonukshe (UGent) , et al.
(2011) CURRENT BIOLOGY. 21(12). p.1055-1060
Author
Organization
Abstract
The polarized transport of the phytohormone auxin [1], which is crucial for the regulation of different stages of plant development [2, 3], depends on the asymmetric plasma membrane distribution of the PIN-FORMED (PIN) auxin efflux carriers [4, 5]. The PIN polar localization results from clathrin-mediated endocytosis (CME) from the plasma membrane and subsequent polar recycling [6]. The Arabidopsis genome encodes two groups of dynamin-related proteins (DRPs) that show homology to mammalian dynamin-a protein required for fission of endocytic vesicles during CME [7, 8]. Here we show by coimmunoprecipitation (coIP), bimolecular fluorescence complementation (BiFC), and Forster resonance energy transfer (FRET) that members of the DRP1 group closely associate with PIN proteins at the cell plate. Localization and phenotypic analysis of novel drp1 mutants revealed a requirement for DRP1 function in correct PIN distribution and in auxin-mediated development. We propose that rapid and specific internalization of PIN proteins mediated by the DRP1 proteins and the associated CME machinery from the cell plate membranes during cytokinesis is an important mechanism for proper polar PIN positioning in interphase cells.
Keywords
PLANT DEVELOPMENT, PLASMA-MEMBRANE, ENDOCYTOSIS, CYTOKINESIS, GROWTH, FAMILY, AUXIN, DYNAMIN

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MLA
Mravec, Jozef, et al. “Cell Plate Restricted Association of DRP1A and PIN Proteins Is Required for Cell Polarity Establishment in Arabidopsis.” CURRENT BIOLOGY, vol. 21, no. 12, 2011, pp. 1055–60, doi:10.1016/j.cub.2011.05.018.
APA
Mravec, J., Petrasek, J., Li, N., Boeren, S., Karlova, R., Kitakura, S., … Friml, J. (2011). Cell plate restricted association of DRP1A and PIN proteins is required for cell polarity establishment in Arabidopsis. CURRENT BIOLOGY, 21(12), 1055–1060. https://doi.org/10.1016/j.cub.2011.05.018
Chicago author-date
Mravec, Jozef, Jan Petrasek, Na Li, Sjef Boeren, Rumyana Karlova, Saeko Kitakura, Markéta Parezova, et al. 2011. “Cell Plate Restricted Association of DRP1A and PIN Proteins Is Required for Cell Polarity Establishment in Arabidopsis.” CURRENT BIOLOGY 21 (12): 1055–60. https://doi.org/10.1016/j.cub.2011.05.018.
Chicago author-date (all authors)
Mravec, Jozef, Jan Petrasek, Na Li, Sjef Boeren, Rumyana Karlova, Saeko Kitakura, Markéta Parezova, Satoshi Naramoto, Tomasz Nodzyński, Pankaj Dhonukshe, Sebastian Bednarek, Eva Zazimalova, Sacco de Vries, and Jiri Friml. 2011. “Cell Plate Restricted Association of DRP1A and PIN Proteins Is Required for Cell Polarity Establishment in Arabidopsis.” CURRENT BIOLOGY 21 (12): 1055–1060. doi:10.1016/j.cub.2011.05.018.
Vancouver
1.
Mravec J, Petrasek J, Li N, Boeren S, Karlova R, Kitakura S, et al. Cell plate restricted association of DRP1A and PIN proteins is required for cell polarity establishment in Arabidopsis. CURRENT BIOLOGY. 2011;21(12):1055–60.
IEEE
[1]
J. Mravec et al., “Cell plate restricted association of DRP1A and PIN proteins is required for cell polarity establishment in Arabidopsis,” CURRENT BIOLOGY, vol. 21, no. 12, pp. 1055–1060, 2011.
@article{1864515,
  abstract     = {{The polarized transport of the phytohormone auxin [1], which is crucial for the regulation of different stages of plant development [2, 3], depends on the asymmetric plasma membrane distribution of the PIN-FORMED (PIN) auxin efflux carriers [4, 5]. The PIN polar localization results from clathrin-mediated endocytosis (CME) from the plasma membrane and subsequent polar recycling [6]. The Arabidopsis genome encodes two groups of dynamin-related proteins (DRPs) that show homology to mammalian dynamin-a protein required for fission of endocytic vesicles during CME [7, 8]. Here we show by coimmunoprecipitation (coIP), bimolecular fluorescence complementation (BiFC), and Forster resonance energy transfer (FRET) that members of the DRP1 group closely associate with PIN proteins at the cell plate. Localization and phenotypic analysis of novel drp1 mutants revealed a requirement for DRP1 function in correct PIN distribution and in auxin-mediated development. We propose that rapid and specific internalization of PIN proteins mediated by the DRP1 proteins and the associated CME machinery from the cell plate membranes during cytokinesis is an important mechanism for proper polar PIN positioning in interphase cells.}},
  author       = {{Mravec, Jozef and Petrasek, Jan and Li, Na and Boeren, Sjef and Karlova, Rumyana and Kitakura, Saeko and Parezova, Markéta and Naramoto, Satoshi and Nodzyński, Tomasz and Dhonukshe, Pankaj and Bednarek, Sebastian and Zazimalova, Eva and de Vries, Sacco and Friml, Jiri}},
  issn         = {{0960-9822}},
  journal      = {{CURRENT BIOLOGY}},
  keywords     = {{PLANT DEVELOPMENT,PLASMA-MEMBRANE,ENDOCYTOSIS,CYTOKINESIS,GROWTH,FAMILY,AUXIN,DYNAMIN}},
  language     = {{eng}},
  number       = {{12}},
  pages        = {{1055--1060}},
  title        = {{Cell plate restricted association of DRP1A and PIN proteins is required for cell polarity establishment in Arabidopsis}},
  url          = {{http://doi.org/10.1016/j.cub.2011.05.018}},
  volume       = {{21}},
  year         = {{2011}},
}
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