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Clathrin mediates endocytosis and polar distribution of PIN auxin transporters in Arabidopsis

Saeko Kitakura UGent, Steffen Vanneste UGent, Stephanie Robert UGent, Christian Löfke, Thomas Teichmann, Hirokazu Tanaka UGent and Jiri Friml UGent (2011) PLANT CELL. 23(5). p.1920-1931
abstract
Endocytosis is a crucial mechanism by which eukaryotic cells internalize extracellular and plasma membrane material, and it is required for a multitude of cellular and developmental processes in unicellular and multicellular organisms. In animals and yeast, the best characterized pathway for endocytosis depends on the function of the vesicle coat protein clathrin. Clathrin-mediated endocytosis has recently been demonstrated also in plant cells, but its physiological and developmental roles remain unclear. Here, we assessed the roles of the clathrin-mediated mechanism of endocytosis in plants by genetic means. We interfered with clathrin heavy chain (CHC) function through mutants and dominant-negative approaches in Arabidopsis thaliana and established tools to manipulate clathrin function in a cell type-specific manner. The chc2 single mutants and dominant-negative CHC1 (HUB) transgenic lines were defective in bulk endocytosis as well as in internalization of prominent plasma membrane proteins. Interference with clathrin-mediated endocytosis led to defects in constitutive endocytic recycling of PIN auxin transporters and their polar distribution in embryos and roots. Consistent with this, these lines had altered auxin distribution patterns and associated auxin transport-related phenotypes, such as aberrant embryo patterning, imperfect cotyledon specification, agravitropic growth, and impaired lateral root organogenesis. Together, these data demonstrate a fundamental role for clathrin function in cell polarity, growth, patterning, and organogenesis in plants.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
PLASMA-MEMBRANE, VESICLE FORMATION, RECEPTOR KINASE, POLLEN TUBES, BREFELDIN-A, ROOT-CELLS, TRAFFICKING, EFFLUX, FEEDBACK-REGULATION, DEPENDENT ENDOCYTOSIS
journal title
PLANT CELL
Plant Cell
volume
23
issue
5
pages
1920 - 1931
Web of Science type
Article
Web of Science id
000292079800019
JCR category
PLANT SCIENCES
JCR impact factor
8.987 (2011)
JCR rank
5/189 (2011)
JCR quartile
1 (2011)
ISSN
1040-4651
DOI
10.1105/tpc.111.083030
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1864472
handle
http://hdl.handle.net/1854/LU-1864472
date created
2011-08-01 17:40:01
date last changed
2012-12-17 16:47:53
@article{1864472,
  abstract     = {Endocytosis is a crucial mechanism by which eukaryotic cells internalize extracellular and plasma membrane material, and it is required for a multitude of cellular and developmental processes in unicellular and multicellular organisms. In animals and yeast, the best characterized pathway for endocytosis depends on the function of the vesicle coat protein clathrin. Clathrin-mediated endocytosis has recently been demonstrated also in plant cells, but its physiological and developmental roles remain unclear. Here, we assessed the roles of the clathrin-mediated mechanism of endocytosis in plants by genetic means. We interfered with clathrin heavy chain (CHC) function through mutants and dominant-negative approaches in Arabidopsis thaliana and established tools to manipulate clathrin function in a cell type-specific manner. The chc2 single mutants and dominant-negative CHC1 (HUB) transgenic lines were defective in bulk endocytosis as well as in internalization of prominent plasma membrane proteins. Interference with clathrin-mediated endocytosis led to defects in constitutive endocytic recycling of PIN auxin transporters and their polar distribution in embryos and roots. Consistent with this, these lines had altered auxin distribution patterns and associated auxin transport-related phenotypes, such as aberrant embryo patterning, imperfect cotyledon specification, agravitropic growth, and impaired lateral root organogenesis. Together, these data demonstrate a fundamental role for clathrin function in cell polarity, growth, patterning, and organogenesis in plants.},
  author       = {Kitakura, Saeko and Vanneste, Steffen and Robert, Stephanie and L{\"o}fke, Christian and Teichmann, Thomas and Tanaka, Hirokazu and Friml, Jiri},
  issn         = {1040-4651},
  journal      = {PLANT CELL},
  keyword      = {PLASMA-MEMBRANE,VESICLE FORMATION,RECEPTOR KINASE,POLLEN TUBES,BREFELDIN-A,ROOT-CELLS,TRAFFICKING,EFFLUX,FEEDBACK-REGULATION,DEPENDENT ENDOCYTOSIS},
  language     = {eng},
  number       = {5},
  pages        = {1920--1931},
  title        = {Clathrin mediates endocytosis and polar distribution of PIN auxin transporters in Arabidopsis},
  url          = {http://dx.doi.org/10.1105/tpc.111.083030},
  volume       = {23},
  year         = {2011},
}

Chicago
Kitakura, Saeko, Steffen Vanneste, Stephanie Robert, Christian Löfke, Thomas Teichmann, Hirokazu Tanaka, and Jiri Friml. 2011. “Clathrin Mediates Endocytosis and Polar Distribution of PIN Auxin Transporters in Arabidopsis.” Plant Cell 23 (5): 1920–1931.
APA
Kitakura, S., Vanneste, S., Robert, S., Löfke, C., Teichmann, T., Tanaka, H., & Friml, J. (2011). Clathrin mediates endocytosis and polar distribution of PIN auxin transporters in Arabidopsis. PLANT CELL, 23(5), 1920–1931.
Vancouver
1.
Kitakura S, Vanneste S, Robert S, Löfke C, Teichmann T, Tanaka H, et al. Clathrin mediates endocytosis and polar distribution of PIN auxin transporters in Arabidopsis. PLANT CELL. 2011;23(5):1920–31.
MLA
Kitakura, Saeko, Steffen Vanneste, Stephanie Robert, et al. “Clathrin Mediates Endocytosis and Polar Distribution of PIN Auxin Transporters in Arabidopsis.” PLANT CELL 23.5 (2011): 1920–1931. Print.